anti-Cat (Feline) Tumor Necrosis Factor antibody for Western Blotting

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Epitope
N-Term
51
44
29
26
24
22
19
17
15
11
11
10
10
8
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
1258
461
356
143
135
118
117
62
54
41
29
17
14
12
9
7
6
4
4
4
3
2
1
1
1
1
Host
Mouse
936
523
184
46
10
9
7
7
5
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Alexa Fluor 488
168
100
74
50
49
31
30
28
27
25
25
25
25
25
24
24
24
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
892
854
625
322
287
229
221
189
137
118
97
69
29
29
26
22
20
18
14
9
9
7
6
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
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Catalog No. ABIN4361078
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4361090 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361083 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361080 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361091 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361092 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361079 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361084 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361085 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361087 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361088 WB PerCP Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361077 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361007 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgM kappa Log in to see 4C6-H8
1 ABIN4361028 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgM kappa Log in to see P-T2
1 ABIN4361032 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgM kappa Log in to see P-T2
1 ABIN4361029 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgM kappa Log in to see P-T2
1 ABIN4361000 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgM kappa Log in to see 4C6-H8
1 ABIN4360999 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgM kappa Log in to see 4C6-H8
1 ABIN4360993 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgM kappa Log in to see 4C6-H8
1 ABIN4360994 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgM kappa Log in to see 4C6-H8
1 ABIN4361003 WB PerCP Mouse IgM kappa Log in to see 4C6-H8

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope N-Term
(51), (44), (29), (26), (24), (22), (19), (17), (15), (11), (11), (10), (10), (8), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(1258), (461), (356), (143), (135), (118), (117), (62), (54), (41), (29), (17), (14), (12), (9), (7), (6), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Host Mouse
(936), (523), (184), (46), (10), (9), (7), (7), (5), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to Alexa Fluor 488
(168), (100), (74), (50), (49), (31), (30), (28), (27), (25), (25), (25), (25), (25), (24), (24), (24), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(892), (854), (625), (322), (287), (229), (221), (189), (137), (118), (97), (69), (29), (29), (26), (22), (20), (18), (14), (9), (9), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
Purification Protein A purified
Immunogen Recombinant N-terminal fragment of human TNF-alpha
Clone TNF706
Isotype IgM kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Molecular Weight Theoretical MW: 17 kDa
Gene ID 7124
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-FrozenImmunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Buffer 50 mM Sodium Borate
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C in the dark.