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anti-Cow (Bovine) Tumor Necrosis Factor antibody for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Cow (Bovine)
1229
445
358
143
136
119
119
60
52
41
29
18
13
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Mouse
927
486
182
46
10
9
7
6
3
2
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
162
96
71
50
32
30
27
26
25
25
25
25
25
25
24
24
24
20
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
864
849
597
311
273
230
202
181
137
118
99
69
27
26
25
22
20
16
14
8
7
6
6
6
6
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN120182
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN598279 FACS ELISA Biotin Mouse IgG2a Log in to see
1 ABIN213085 ELISA FACS Biotin Mouse IgG2a Log in to see
1 ABIN2476861 FACS Alexa Fluor 488 Mouse IgG2b Log in to see CC327 4
1 ABIN2476860 FACS ELISA Mouse IgG2b Log in to see CC327 4
1 ABIN2380287 FACS ELISA Biotin Mouse IgG2a Log in to see 10F545
1 ABIN217729 FACS ELISA Mouse IgG2b Log in to see
1 ABIN2377360 FACS ELISA Mouse IgG2b Log in to see
1 ABIN639287 FACS ELISA Biotin Mouse IgG2a Log in to see
1 ABIN639286 FACS ELISA Mouse IgG2b Log in to see

Top referenced anti-Tumor Necrosis Factor antibody for Flow Cytometry

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Cow (Bovine)
ELISA 38 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Neutralization (Neut) 1 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 36 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Cow (Bovine)
(1229), (445), (358), (143), (136), (119), (119), (60), (52), (41), (29), (18), (13), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Mouse
(927), (486), (182), (46), (10), (9), (7), (6), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(162), (96), (71), (50), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (20), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
(864), (849), (597), (311), (273), (230), (202), (181), (137), (118), (99), (69), (27), (26), (25), (22), (20), (16), (14), (8), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN120182) Images
Specificity This antibody recognises Bovine TNF alpha.
No Cross-Reactivity Sheep (Ovine)
Cross-Reactivity (Details) Species reactivity (tested):Bovine.
Purification Affinity Chromatography on Protein G.
Immunogen Recombinant bovine TNF alpha.
Clone CC327
Isotype IgG2b

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN120182) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background Tumor Necrosis Factor alpha (TNF alpha) is a 17.5 kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes. The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 280943
NCBI Accession NP_776391
UniProt Q06599
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN120182) Images back to top
Application Notes Flow Cytometry: Membrane permeabilisation is required for this application. ELISA: This reagent may be used as a capture antibody in ELISA assays for bovine and sheep TNF alpha in combination with ABIN120216 as detection reagent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN120182) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Tumor Necrosis Factor Antibody (ABIN120182)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Studer, Collette, Whelan, Albrecht, Casselman, de Reijke, Knoenagel, Loidl, Isorna, Sundaram, Debois,: "Using PSA to Guide Timing of Androgen Deprivation in Patients with T0-4 N0-2 M0 Prostate Cancer not Suitable for Local Curative Treatment (EORTC 30891)." in: European urology, 2008

Background publications

Hope, Whelan, Hewinson, Vordermeier, Howard: "Maturation of bovine dendritic cells by lipopeptides." in: Veterinary immunology and immunopathology, Vol. 95, Issue 1-2, pp. 21-31, 2003

Whelan, Hope, Howard, Clifford, Hewinson, Vordermeier: "Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide." in: Infection and immunity, Vol. 71, Issue 11, pp. 6420-5, 2003

Guergnon, Chaussepied, Sopp, Lizundia, Moreau, Blumen, Werling, Howard, Langsley: "A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaB activation of Theileria parva-transformed B cells." in: Cellular microbiology, Vol. 5, Issue 10, pp. 709-16, 2003