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|Application / Reactivity||Cow (Bovine)|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||11 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||1 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Western Blotting (WB)||35 Antibodies|
|Antigen||Tumor Necrosis Factor (TNF) Antibodies|
|Reactivity||Cow (Bovine) Alternatives|
|Conjugate||This Tumor Necrosis Factor antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
|4 references available|
|Supplier||Log in to see|
Product Details anti-Tumor Necrosis Factor AntibodyTarget Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN120182) Images
|Specificity||This antibody recognises Bovine TNF alpha.|
|No Cross-Reactivity||Sheep (Ovine)|
|Cross-Reactivity (Details)||Species reactivity (tested):Bovine.|
|Purification||Affinity Chromatography on Protein G.|
|Immunogen||Recombinant bovine TNF alpha.|
Target Details Tumor Necrosis FactorProduct Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN120182) Images back to top
|Alternative Name||Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)|
|Background||Tumor Necrosis Factor alpha (TNF alpha) is a 17.5 kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes. The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2|
|Research Area||Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN120182) Images back to top
Flow Cytometry: Membrane permeabilisation is required for this application. ELISA: This reagent may be used as a capture antibody in ELISA assays for bovine and sheep TNF alpha in combination with ABIN120216 as detection reagent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN120182) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-Tumor Necrosis Factor Antibody (ABIN120182)Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
|Product cited in:||
Studer, Collette, Whelan et al.: "Using PSA to Guide Timing of Androgen Deprivation in Patients with T0-4 N0-2 M0 Prostate Cancer not Suitable for Local Curative Treatment (EORTC 30891)." in: European urology, 2008 (PubMed).
Whelan, Hope, Howard et al.: "Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide." in: Infection and immunity, Vol. 71, Issue 11, pp. 6420-5, 2003 (PubMed).
Hope, Whelan, Hewinson et al.: "Maturation of bovine dendritic cells by lipopeptides." in: Veterinary immunology and immunopathology, Vol. 95, Issue 1-2, pp. 21-31, 2003 (PubMed).
Guergnon, Chaussepied, Sopp et al.: "A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaB activation of Theileria parva-transformed B cells." in: Cellular microbiology, Vol. 5, Issue 10, pp. 709-16, 2003 (PubMed).