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anti-Dog (Canine) Tumor Necrosis Factor antibody for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Epitope
N-Term
36
30
27
25
24
23
18
17
13
12
10
10
10
7
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
1242
465
346
143
136
122
122
56
56
45
31
18
8
8
7
7
4
4
4
3
2
2
2
1
1
1
Host
Mouse
958
480
213
43
13
9
9
8
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
99
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
882
804
581
258
255
167
146
145
135
124
104
63
25
25
24
22
22
19
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN4360942
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010401985 ABIN4360941 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.0010401985 ABIN4360943 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.0010401985 ABIN3026343 FACS IF IHC Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8
0.0010401985 ABIN4360935 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8
0.0010401985 ABIN4360936 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8
0.0010401985 ABIN4360944 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM Log in to see TNF706-P-T2
0.0010401985 ABIN4360957 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see SPM543
0.0010401985 ABIN4360940 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see P-T2
0.0010401985 ABIN4360971 ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.0010401985 ABIN4360958 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see SPM543
0.0010401985 ABIN4360972 ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.0010401985 ABIN4360977 FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM543
0.0010401985 ABIN4360979 FACS ICC IF IHC (fro) IHC (p) IP Allophycocyanin Mouse IgG1 kappa Log in to see SPM543
0.0010401985 ABIN4360981 FACS ICC IF IHC (fro) IHC (p) IP DyLight 650 Mouse IgG1 kappa Log in to see SPM543
0.0010401985 ABIN4360982 FACS ICC IF IHC (fro) IHC (p) IP DyLight 680 Mouse IgG1 kappa Log in to see SPM543
0.0010401985 ABIN4360983 FACS ICC IF IHC (fro) IHC (p) IP DyLight 488 Mouse IgG1 kappa Log in to see SPM543
0.0010401985 ABIN4360989 FACS ICC IF IHC (fro) IHC (p) IP DyLight 350 Mouse IgG1 kappa Log in to see SPM543
0.0010401985 ABIN4360990 FACS ICC IF IHC (fro) IHC (p) IP DyLight 405 Mouse IgG1 kappa Log in to see SPM543
0.0010401985 ABIN4360988 FACS ICC IF IHC (fro) IHC (p) IP DyLight 550 Mouse IgG1 kappa Log in to see SPM543
0.0010401985 ABIN4360991 FACS ICC IF IHC (fro) IHC (p) IP DyLight 405LS Mouse IgG1 kappa Log in to see SPM543

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Rabbit Cat (Feline) Zebrafish (Danio rerio)
Western Blotting (WB) 596 Antibodies 260 Antibodies 163 Antibodies 77 Antibodies 78 Antibodies 69 Antibodies 69 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Radioimmunoassay (RIA) 6 Antibodies 17 Antibodies
Protein Assay (PrA) 2 Antibodies
Neutralization (Neut) 73 Antibodies 55 Antibodies 21 Antibodies 1 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope N-Term
(36), (30), (27), (25), (24), (23), (18), (17), (13), (12), (10), (10), (10), (7), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(1242), (465), (346), (143), (136), (122), (122), (56), (56), (45), (31), (18), (8), (8), (7), (7), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(958), (480), (213), (43), (13), (9), (9), (8), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (99), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(882), (804), (581), (258), (255), (167), (146), (145), (135), (124), (104), (63), (25), (25), (24), (22), (22), (19), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
Purification Protein A purified
Immunogen Recombinant N-terminal fragment of human TNF-alpha
Clone TNF706
Isotype IgM kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Molecular Weight Theoretical MW: 17 kDa
UniProt P01375
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Flow Cytometry 0.5-1 μg/million cells, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1-2 μg/500 μg protein, Immunohistochemistry-Paraffin 1-2 μg/mL, Immunohistochemistry-Frozen 1-2 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.