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anti-Dog (Canine) Tumor Necrosis Factor antibody for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Epitope
N-Term
37
30
26
24
23
23
18
16
13
12
10
10
10
7
4
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
1214
441
335
138
131
117
106
55
55
31
26
18
13
8
8
7
7
4
4
4
3
2
2
1
1
1
Host
Mouse
918
487
201
44
13
9
7
6
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
168
95
69
49
32
30
27
26
25
25
25
25
25
25
24
24
24
18
13
9
4
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
891
807
593
306
255
237
205
182
129
128
101
63
27
25
22
22
18
14
8
8
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4360943
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4360941 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4360942 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361087 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361089 FACS PE Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361090 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361091 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361092 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361077 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361078 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361079 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361080 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361081 FACS Allophycocyanin Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361083 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361085 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361084 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN3023987 FACS IF IHC (p) Mouse IgM, kappa AA 115-130 Log in to see P-T2
1 ABIN3023960 FACS IF IHC (p) Mouse IgM AA 115-130, N-Term Log in to see TNF706-P-T2
1 ABIN3023991 FACS IF IHC Mouse IgG1, kappa AA 115-130 Log in to see SPM543
1 ABIN4360945 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM Log in to see TNF706-P-T2
1 ABIN4360946 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM Log in to see TNF706-P-T2

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human Cat (Feline) Dog (Canine) Mouse (Murine) Rabbit Rat (Rattus) Zebrafish (Danio rerio)
Activity Inhibition (Act Inh) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 27 Antibodies 10 Antibodies 10 Antibodies 10 Antibodies 10 Antibodies 18 Antibodies 10 Antibodies
Immunohistochemistry (Free Floating) (IHC (ff)) 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 521 Antibodies 241 Antibodies 142 Antibodies 57 Antibodies 58 Antibodies 49 Antibodies 49 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Radioimmunoassay (RIA) 6 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope N-Term
(37), (30), (26), (24), (23), (23), (18), (16), (13), (12), (10), (10), (10), (7), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(1214), (441), (335), (138), (131), (117), (106), (55), (55), (31), (26), (18), (13), (8), (8), (7), (7), (4), (4), (4), (3), (2), (2), (1), (1), (1)
Host Mouse
(918), (487), (201), (44), (13), (9), (7), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(168), (95), (69), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (18), (13), (9), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(891), (807), (593), (306), (255), (237), (205), (182), (129), (128), (101), (63), (27), (25), (22), (22), (18), (14), (8), (8), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
Purification Protein A purified
Immunogen Recombinant N-terminal fragment of human TNF-alpha
Clone TNF706
Isotype IgM kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Molecular Weight Theoretical MW: 17 kDa
Gene ID 7124
UniProt P01375
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Flow Cytometry 0.5-1 μg/million cells, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1-2 μg/500 μg protein, Immunohistochemistry-Paraffin 1-2 μg/mL, Immunohistochemistry-Frozen 1-2 μg/mLELISA (For coating, order NBP2-34728 (BSA free version of NBP2-34303))
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.