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anti-Ferret Tumor Necrosis Factor antibody for ELISA

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Epitope
AA 77-233
37
29
28
25
23
23
18
16
13
12
10
10
10
7
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Ferret
1233
462
344
144
138
123
123
56
52
45
31
18
8
7
5
4
4
3
3
3
3
2
2
1
1
1
Host
Mouse
952
479
210
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
98
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Minimum Detection Limit
0.078 ng/well
Application
ELISA
880
796
577
252
249
163
145
142
135
121
103
63
25
25
23
22
22
17
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2001463
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012100277 ABIN2693121 ELISA Rabbit Log in to see Polyclonal
0.0012100277 ABIN2693119 ELISA Rabbit Log in to see Polyclonal

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Ferret
ELISA 3 Antibodies
Western Blotting (WB) 1 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 77-233
(37), (29), (28), (25), (23), (23), (18), (16), (13), (12), (10), (10), (10), (7), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Ferret
(1233), (462), (344), (144), (138), (123), (123), (56), (52), (45), (31), (18), (8), (7), (5), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(952), (479), (210), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (98), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Minimum Detection Limit 0.078 ng/well
Application ELISA
(880), (796), (577), (252), (249), (163), (145), (142), (135), (121), (103), (63), (25), (25), (23), (22), (22), (17), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity Ferret TNFA / TNF-alpha
Cross-Reactivity (Details) Has cross-reactivity in ELISA with Canine TNFα No cross-reactivity in ELISA with Human TNFα Mouse TNFα E.coli cell lysate
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret TNFA / TNF-alpha (rF TNFA / TNF-alpha, A3FBF1, Val 77-Leu 233). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification Affinity Purified
Sterility 0.2 μm filtered
Immunogen Recombinant Ferret TNFA / TNF-alpha protein
Clone 11
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF (TNF Antibody Abstract)
Background Tumor necrosis factor alpha (TNFa), also known as cachectin and TNFSF1A, is the prototypic cytokine of the the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNFa is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNFa is primarily produced as a 26 kDa type II transmembrane protein arranged in stable homotrimers, and a 55 kDa soluble trimeric form of TNFa is released via proteolytic cleavage by the metalloprotease TACE/ ADAM17. Two receptors mediating the TNFa function have been identified, the ubiquitous TNFRI from which most signaling are derived, and the hematopoietic cell-restricted TNFRII. Upon the TNFa trimers binding, TNF receptors also form trimers, undergo a conformational change, and enable the adaptor protein TRADD to bind to the death domain, and thus initiates the signal pathways such as the NF-kB, Jak/STAT, and the MAPK pathways, as well as the death signaling. As a major mediator of apoptosis, inflammation and immunity, TNFa has been implicated in the pathogenesis of a variety of diseases including autoimmune diseases, insulin resistance, and cancer.
UniProt A3FBF1
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Direct ELISA - This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Ferret TNFα. The detection limit for Ferret TNFα is approximately 0.078 ng/well.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Buffer PBS with 5% trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C/-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -70°C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Expiry Date 12 months