anti-Ferret Tumor Necrosis Factor antibody for ELISA

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Epitope
AA 77-233
51
43
29
27
24
22
17
17
15
11
11
10
10
8
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Ferret
1251
461
359
145
136
119
119
63
55
41
29
18
14
12
9
7
5
4
4
4
4
2
1
1
1
1
Host
Mouse
934
519
184
48
10
9
7
6
4
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
169
100
74
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, ELISA (Detection)
886
858
622
321
287
230
219
190
138
118
97
69
29
29
26
22
20
18
14
9
7
7
6
6
6
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN2001463
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2693121 ELISA Rabbit IgG AA 77-233 Log in to see Polyclonal
1 ABIN2693119 ELISA Rabbit IgG AA 77-233 Log in to see Polyclonal
1 ABIN5508604 ELISA Rabbit IgG AA 77-233 Log in to see 030

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 77-233
(51), (43), (29), (27), (24), (22), (17), (17), (15), (11), (11), (10), (10), (8), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Ferret
(1251), (461), (359), (145), (136), (119), (119), (63), (55), (41), (29), (18), (14), (12), (9), (7), (5), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Mouse
(934), (519), (184), (48), (10), (9), (7), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(169), (100), (74), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, ELISA (Detection)
(886), (858), (622), (321), (287), (230), (219), (190), (138), (118), (97), (69), (29), (29), (26), (22), (20), (18), (14), (9), (7), (7), (6), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity Ferret TNFA / TNF-alpha
Has cross-reactivity in ELISA with
Canine TNFα
No cross-reactivity in ELISA with
Human TNFα
Mouse TNFα
E.coli cell lysate
Cross-Reactivity Dog (Canine)
No Cross-Reactivity Human, Mouse (Murine), Escherichia coli (E. coli)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret TNFA / TNF-alpha (rF TNFA / TNF-alpha, ABIN2008788 A3FBF1, Val 77-Leu 233,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Ferret TNFA / TNF-alpha protein (ABIN2008788)
Clone 11
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF (TNF Antibody Abstract)
Background Tumor necrosis factor alpha (TNFa), also known as cachectin and TNFSF1A, is the prototypic cytokine of the the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNFa is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNFa is primarily produced as a 26 kDa type II transmembrane protein arranged in stable homotrimers, and a 55 kDa soluble trimeric form of TNFa is released via proteolytic cleavage by the metalloprotease TACE/ ADAM17. Two receptors mediating the TNFa function have been identified, the ubiquitous TNFRI from which most signaling are derived, and the hematopoietic cell-restricted TNFRII. Upon the TNFa trimers binding, TNF receptors also form trimers, undergo a conformational change, and enable the adaptor protein TRADD to bind to the death domain, and thus initiates the signal pathways such as the NF-kB, Jak/STAT, and the MAPK pathways, as well as the death signaling. As a major mediator of apoptosis, inflammation and immunity, TNFa has been implicated in the pathogenesis of a variety of diseases including autoimmune diseases, insulin resistance, and cancer.
UniProt A3FBF1
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Ferret TNFα. The detection limit for Ferret TNFα is approximately 0.078 ng/well.
ELISA(Det): 0.1-1 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months