|Application / Reactivity||Human|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Antibody (Inhibition)||13 Antibodies|
|Blocking Reagent (BR)||2 Antibodies|
|Cellular Assay (CA)||2 Antibodies|
|Dot Blot (DB)||4 Antibodies|
|ELISA (Capture)||6 Antibodies|
|ELISA (Detection)||4 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||3 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||2 Antibodies|
|Enzyme Immunoassay (EIA)||42 Antibodies|
|Flow Cytometry (FACS)||217 Antibodies|
|Functional Studies (Func)||68 Antibodies|
|Immunoassay (IA)||13 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||31 Antibodies|
|Immunofluorescence (IF)||50 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||24 Antibodies|
|Antigen||Tumor Necrosis Factor (TNF) Antibodies|
|Conjugate||This Tumor Necrosis Factor antibody is un-conjugated Alternatives|
Biochemical Assay (BCA)
|10 references available|
|Supplier||Log in to see|
Product Details anti-Tumor Necrosis Factor AntibodyTarget Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2665427) Images
|Cross-Reactivity||Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Pig (Porcine), Pigtailed Macaque, Sooty mangabey (Cercocebus atys)|
|Purification||The antibody was purified by affinity chromatography.|
Target Details Tumor Necrosis FactorProduct Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2665427) Images back to top
|Alternative Name||TNF-alpha (TNF Antibody Abstract)|
|Background||TNF-α is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes, stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.|
|Research Area||Inflammation, Cancer, Virology|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2665427) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN2665427) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and EDTA.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-Tumor Necrosis Factor Antibody (ABIN2665427)Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Iwamoto, Iwai, Tsujiyama et al.: "TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1449-57, 2007 (PubMed).
Chen, Hou, Jiang et al.: "Response of memory CD8+ T cells to severe acute respiratory syndrome (SARS) coronavirus in recovered SARS patients and healthy individuals." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 1, pp. 591-8, 2005 (PubMed).
Enríquez, Klínger, Arturo et al.: "Peritonitis in continuous ambulatory peritoneal dialysis: cytokines in peritoneal fluid and blood." in: Advances in peritoneal dialysis. Conference on Peritoneal Dialysis, Vol. 18, pp. 177-83, 2002 (PubMed).
Andersson, Wang, Palmblad et al.: "High mobility group 1 protein (HMG-1) stimulates proinflammatory cytokine synthesis in human monocytes." in: The Journal of experimental medicine, Vol. 192, Issue 4, pp. 565-70, 2001 (PubMed).
Moormann, Sullivan, Rochford et al.: "Malaria and pregnancy: placental cytokine expression and its relationship to intrauterine growth retardation." in: The Journal of infectious diseases, Vol. 180, Issue 6, pp. 1987-93, 2000 (PubMed).
Tracey, Cerami: "Tumor necrosis factor: an updated review of its biology." in: Critical care medicine, Vol. 21, Issue 10 Suppl, pp. S415-22, 1993 (PubMed).
Beutler, Cerami: "Tumor necrosis, cachexia, shock, and inflammation: a common mediator." in: Annual review of biochemistry, Vol. 57, pp. 505-18, 1988 (PubMed).
Beutler, Cerami: "The biology of cachectin/TNF--a primary mediator of the host response." in: Annual review of immunology, Vol. 7, pp. 625-55, 1989 (PubMed).
Danis, Franic, Rathjen et al.: "Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 ..." in: Clinical and experimental immunology, Vol. 85, Issue 1, pp. 143-50, 1991 (PubMed).
Rathjen, Cowan, Furphy et al.: "Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors." in: Molecular immunology, Vol. 28, Issue 1-2, pp. 79-86, 1991 (PubMed).
ImagesProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2665427) back to top