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anti-Human Tumor Necrosis Factor antibody for Biochemical Assay

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Human
1228
445
358
143
136
119
119
60
53
41
29
18
13
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Mouse
927
486
182
46
10
9
7
6
3
2
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
162
96
71
50
32
30
27
26
25
25
25
25
25
25
24
24
24
20
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Biochemical Assay (BCA)
864
849
598
311
273
230
202
181
137
118
99
70
27
26
25
22
20
16
14
8
7
6
6
6
6
5
5
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2665427
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Top referenced anti-Tumor Necrosis Factor antibody for Biochemical Assay

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human
Activity Inhibition (Act Inh) 2 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Peptide (BP) 5 Antibodies
Blocking Reagent (BR) 5 Antibodies
Cell Culture (CC) 1 Antibodies
Cellular Assay (CA) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 27 Antibodies
Dot Blot (DB) 2 Antibodies
ELISA 580 Antibodies
ELISA (Capture) 5 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 49 Antibodies
Flow Cytometry (FACS) 504 Antibodies
Functional Studies (Func) 69 Antibodies
Immunoassay (IA) 13 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1228), (445), (358), (143), (136), (119), (119), (60), (53), (41), (29), (18), (13), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Mouse
(927), (486), (182), (46), (10), (9), (7), (6), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(162), (96), (71), (50), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (20), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Biochemical Assay (BCA)
(864), (849), (598), (311), (273), (230), (202), (181), (137), (118), (99), (70), (27), (26), (25), (22), (20), (16), (14), (8), (7), (6), (6), (6), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2665427) Images
Cross-Reactivity Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Pig (Porcine), Pigtailed Macaque, Sooty mangabey (Cercocebus atys)
Purification The antibody was purified by affinity chromatography.
Clone MAb11
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2665427) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background TNF-α is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes, stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2665427) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN2665427) Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and EDTA.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-Tumor Necrosis Factor Antibody (ABIN2665427)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Iwamoto, Iwai, Tsujiyama, Kurahashi, Takeshita, Naoe, Masunaga, Ogawa, Oguchi, Miyazaki: "TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1449-57, 2007

Chen, Hou, Jiang, Ma, Meng, Wang, Zhang, Zhang, Tang, Zhang, Wan, Li, Yu, Hu, Yang, He, Wang, Cao: "Response of memory CD8+ T cells to severe acute respiratory syndrome (SARS) coronavirus in recovered SARS patients and healthy individuals." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 1, pp. 591-8, 2005

Enríquez, Klínger, Arturo, Delgado, Tobar, Mosquera: "Peritonitis in continuous ambulatory peritoneal dialysis: cytokines in peritoneal fluid and blood." in: Advances in peritoneal dialysis. Conference on Peritoneal Dialysis, Vol. 18, pp. 177-83, 2002

Andersson, Wang, Palmblad, Aveberger, Bloom, Erlandsson-Harris, Janson, Kokkola, Zhang, Yang, Tracey: "High mobility group 1 protein (HMG-1) stimulates proinflammatory cytokine synthesis in human monocytes." in: The Journal of experimental medicine, Vol. 192, Issue 4, pp. 565-70, 2001

Moormann, Sullivan, Rochford, Chensue, Bock, Nyirenda, Meshnick: "Malaria and pregnancy: placental cytokine expression and its relationship to intrauterine growth retardation." in: The Journal of infectious diseases, Vol. 180, Issue 6, pp. 1987-93, 2000

Tracey, Cerami: "Tumor necrosis factor: an updated review of its biology." in: Critical care medicine, Vol. 21, Issue 10 Suppl, pp. S415-22, 1993

Rathjen, Cowan, Furphy, Aston: "Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors." in: Molecular immunology, Vol. 28, Issue 1-2, pp. 79-86, 1991

Danis, Franic, Rathjen, Brooks et al.: "Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 ..." in: Clinical and experimental immunology, Vol. 85, Issue 1, pp. 143-50, 1991

Beutler, Cerami: "The biology of cachectin/TNF--a primary mediator of the host response." in: Annual review of immunology, Vol. 7, pp. 625-55, 1989

Beutler, Cerami: "Tumor necrosis, cachexia, shock, and inflammation: a common mediator." in: Annual review of biochemistry, Vol. 57, pp. 505-18, 1988

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2665427) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN2665427) anti-Tumor Necrosis Factor (TNF) antibody
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN2665427) anti-Tumor Necrosis Factor (TNF) antibody (Image 2)