anti-Human Tumor Necrosis Factor antibody for Cytometry by Time of Flight

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Human
1250
461
359
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Mouse
934
519
184
48
10
9
7
6
4
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
169
100
74
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunofluorescence (IF)
887
858
621
321
287
230
218
190
138
118
97
69
29
28
26
22
20
18
14
9
7
7
6
6
6
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN4361143
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4361142 CyTOF FACS IF Mouse IgG1 kappa Log in to see J1D9
1 ABIN4361243 CyTOF ELISA FACS Rabbit IgG Log in to see cA2 (Infliximab)
1 ABIN4360972 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4360971 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361242 CyTOF ELISA FACS Rabbit IgG Log in to see cA2 (Infliximab)
1 ABIN4361145 CyTOF FACS IF Mouse IgG1 kappa Log in to see J2D10
1 ABIN4964355 CyTOF FACS IF IHC IHC (p) Rabbit IgG Log in to see TNF-1500
1 ABIN4964357 CyTOF FACS IF IHC (p) Rabbit IgG Log in to see TNF-1500
1 ABIN4964354 CyTOF FACS IF IHC IHC (p) Rabbit IgG Log in to see TNF-1500
1 ABIN4964356 CyTOF FACS IF IHC (p) Rabbit IgG Log in to see TNF-1500
1 ABIN4360961 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8
1 ABIN4360962 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8
1 ABIN4360959 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360965 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see P-T2
1 ABIN4361144 CyTOF FACS IF Mouse IgG1 kappa Log in to see J2D10
1 ABIN4360960 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360964 BP CyTOF ELISA FACS ICC IF IHC (fro) IP Neut WB Mouse IgM kappa Log in to see MAb1
1 ABIN4360963 BP CyTOF ELISA FACS ICC IF IHC (fro) IP Neut WB Mouse IgM kappa Log in to see MAb1
1 ABIN4360973 CyTOF FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM Log in to see TNF706-P-T2
1 ABIN4360966 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see P-T2

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1250), (461), (359), (145), (136), (119), (119), (63), (55), (41), (29), (18), (14), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Mouse
(934), (519), (184), (48), (10), (9), (7), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(169), (100), (74), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunofluorescence (IF)
(887), (858), (621), (321), (287), (230), (218), (190), (138), (118), (97), (69), (29), (28), (26), (22), (20), (18), (14), (9), (7), (7), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity This antibody neutralizes TNF alpha biological activities. It prevents TNF alpha induced apoptosis in Jurkat cells. It also neutralizes HurTNFamediated cytotoxicity of L929 cells and inhibits tumor growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
No Cross-Reactivity Mouse (Murine)
Purification Protein A or G purified
Immunogen Recombinant human TNF-alpha
Clone J1D9
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Molecular Weight Theoretical MW: 17 kDa
Gene ID 7124
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Flow Cytometry : 0.5 - 1 μg/million cells in 0.1 ml, Immunofluorescence : 1 - 2 μg/mL, CyTOF-readyHu-chromosome location: 6p21.33 Inhibits Tumor Growth Order Ab without BSA/Azide, Neutralizes rTNF-alpha Mediated Cytotoxicity Order Ab without BSA/Azide, Protects Mice Against Toxicity of rhTNF-alpha Order Ab without BSA/Azide The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: No Preservative
Preservative Without preservative
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.