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anti-Human Tumor Necrosis Factor antibody for ELISpot (Detection)

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human
704
308
142
55
52
31
31
27
23
18
12
12
8
7
5
4
4
4
3
3
2
2
1
1
1
Host
Mouse
537
394
157
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Biotin
146
83
44
24
20
9
9
7
5
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
ELISA (Detection), ELISpot (Detection) (ELISPOT (Detection))
633
587
319
208
120
100
99
86
80
63
56
56
25
25
22
22
15
14
8
8
6
5
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN280020
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000871331 ABIN1721170 ELISA (Detection) ELISPOT (Detection) Biotin Mouse IgG1 Log in to see B-C7

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cellular Assay (CA) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 341 Antibodies
ELISA (Capture) 6 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Enzyme Immunoassay (EIA) 42 Antibodies
Flow Cytometry (FACS) 217 Antibodies
Functional Studies (Func) 68 Antibodies
Immunoassay (IA) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 31 Antibodies
Immunofluorescence (IF) 50 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(704), (308), (142), (55), (52), (31), (31), (27), (23), (18), (12), (12), (8), (7), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(537), (394), (157), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to Biotin
(146), (83), (44), (24), (20), (9), (9), (7), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application ELISA (Detection), ELISpot (Detection) (ELISPOT (Detection))
(633), (587), (319), (208), (120), (100), (99), (86), (80), (63), (56), (56), (25), (25), (22), (22), (15), (14), (8), (8), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF-α ), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.
Characteristics The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF-α ), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4 + T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.
Synonyms: Synonyms: Tumor Necrosis Factor alpha
Purification Purified
Clone MAb11
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNFa (TNF Antibody Abstract)
Background TNF-alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.
Research Area Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Applications reported: The MAb11 antibody has been reported for use in detection of human TNF-α by ELISA.

Applications tested: The MAb11 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human TNF- alpha (TNF-a, TNFa) in combination with the purified MAb1 (14-7348) antibody for capture and recombinant human TNFa (14-8329) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze.
Storage 4 °C

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
 image for anti-Tumor Necrosis Factor antibody (TNF)  (Biotin) (ABIN280020) Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in th...