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anti-Human Tumor Necrosis Factor antibody for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human
704
308
142
55
52
31
31
27
23
18
12
12
8
7
5
4
4
4
3
3
2
2
1
1
1
Host
Mouse
537
394
157
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to PE
147
83
43
24
20
9
9
7
5
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
633
587
318
208
120
100
99
86
80
63
56
56
25
25
22
22
15
14
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN1383957
$ 326.87
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.28944895 ABIN1997163 FACS ELISA Mouse IgG1 AA 77-233 Log in to see 1
0.28944895 ABIN1383956 FACS FITC Mouse IgG1 Log in to see B-D9 1
0.28944895 ABIN2662374 FACS Pacific Blue Mouse IgG1 kappa Log in to see MAb11 11
0.28944895 ABIN2658839 FACS APC Mouse IgG1 kappa Log in to see MAb11 10
0.28944895 ABIN2657286 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see MAb11 10
0.28944895 ABIN2657587 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see MAb11 10
0.28944895 ABIN2659610 FACS PE-Cy7 Mouse IgG1 kappa Log in to see MAb11 10
0.28944895 ABIN2659787 BR FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see MAb11 10
0.28944895 ABIN2662036 FACS FITC Mouse IgG1 kappa Log in to see MAb11 11
0.28944895 ABIN2660627 FACS APC-Cy7 Mouse IgG1 kappa Log in to see MAb11 10
0.28944895 ABIN2660503 FACS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see MAb11 10
0.28944895 ABIN2663940 FACS PE Mouse IgG1 kappa Log in to see MAb11 10
0.28944895 ABIN263070 ICC FACS IF IHC (p) FITC Mouse IgG1 kappa Log in to see MAb11
0.28944895 ABIN1997166 FACS FITC Mouse IgG1 AA 77-233 Log in to see 1
0.28944895 ABIN1997168 FACS FITC Rabbit IgG AA 77-233 Log in to see 101
0.28944895 ABIN2497050 FACS WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.28944895 ABIN2681098 FACS ICS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see MAb11
0.28944895 ABIN2681100 FACS ICS EFluor 450 Mouse IgG1 kappa Log in to see MAb11
0.28944895 ABIN2681104 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see MAb11
0.28944895 ABIN2681102 FACS ICS Alexa Fluor 488 Mouse IgG1 kappa Log in to see MAb11

Top referenced anti-Tumor Necrosis Factor antibody for Flow Cytometry

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cellular Assay (CA) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 341 Antibodies
ELISA (Capture) 6 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Enzyme Immunoassay (EIA) 42 Antibodies
Flow Cytometry (FACS) 217 Antibodies
Functional Studies (Func) 68 Antibodies
Immunoassay (IA) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 31 Antibodies
Immunofluorescence (IF) 50 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(704), (308), (142), (55), (52), (31), (31), (27), (23), (18), (12), (12), (8), (7), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(537), (394), (157), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to PE
(147), (83), (43), (24), (20), (9), (9), (7), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(633), (587), (318), (208), (120), (100), (99), (86), (80), (63), (56), (56), (25), (25), (22), (22), (15), (14), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1383957) Images
Specificity Recognises both natural and recombinant TNF-a
Characteristics Recombinant human TNF-a.
Purification Ion exchange chromatography
Clone B-D9
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1383957) Images back to top
Antigen
Alternative Name TNF-a (TNF Antibody Abstract)
Background Myeloma X63/AG.8653 x Balb/c spleen cells
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1383957) Images back to top
Application Notes Suitable for intra-cytoplasmic flow cytometry
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN1383957) Images back to top
Format Liquid
Reconstitution Reconstitute with 1 mL deionized water
Buffer Phosphate-buffered saline with 5 % BSA and 0,1 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice It is recommended to establish the optimal dilution according to the system being used. Use 10 µL of (diluted) antibody to label 5 x 10 5 cells. Do not freeze.
Storage 4 °C
Storage Comment Declared stable after 7 days at room temperature, 6 months at +2-8°C after reconstitution. Protect from light. Do not freeze.
Expiry Date 6 months

References for anti-Tumor Necrosis Factor Antibody (ABIN1383957)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Arduise, Abache, Li, Billard, Chabanon, Ludwig, Mauduit, Boucheix, Rubinstein, Le Naour: "Tetraspanins regulate ADAM10-mediated cleavage of TNF-alpha and epidermal growth factor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 10, pp. 7002-13, 2008

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1383957) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor antibody (TNF)  (PE) (ABIN1383957) A typical staining pattern with the B-D9 monoclonal antibody of PMA and ionomycin act...