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anti-Human Tumor Necrosis Factor antibody for Intracellular Staining

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human
1241
465
346
144
138
123
123
56
56
45
31
18
8
8
7
7
4
4
4
3
3
2
2
1
1
1
Host
Mouse
959
480
213
43
13
9
9
8
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
173
99
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
883
805
581
258
255
167
146
145
136
124
104
63
25
24
24
22
22
19
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN281697
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN283061 FACS ICS PE-Cy7 Mouse IgG1 kappa Log in to see MAb11
0.0008709465 ABIN282880 FACS ICS APC Mouse IgG1 kappa Log in to see MAb11
0.0008709465 ABIN1003400 ICS IP ELISA WB Mouse IgG1 Log in to see NYRhTNFa-E2
0.0008709465 ABIN279164 FACS ICS PE Mouse IgG1 kappa Log in to see MAb11
0.0008709465 ABIN278194 FACS ICS FITC Mouse IgG1 kappa Log in to see MAb11
0.0008709465 ABIN2681098 FACS ICS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see MAb11
0.0008709465 ABIN2681102 FACS ICS Alexa Fluor 488 Mouse IgG1 kappa Log in to see MAb11
0.0008709465 ABIN2681100 FACS ICS EFluor 450 Mouse IgG1 kappa Log in to see MAb11
0.0008709465 ABIN2681106 FACS ICS PE-eFluor® 610 Mouse IgG1 kappa Log in to see MAb11
0.0008709465 ABIN2749005 ICS Neut IHC ELISA Mouse IgG1 Log in to see B-C7
0.0008709465 ABIN808120 ICS IP TCC ELISA Mouse Log in to see NYRhTNFa-E2

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cellular Assay (CA) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 501 Antibodies
ELISA (Capture) 6 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Enzyme Immunoassay (EIA) 42 Antibodies
Flow Cytometry (FACS) 473 Antibodies
Functional Studies (Func) 72 Antibodies
Immunoassay (IA) 16 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 96 Antibodies
Immunofluorescence (IF) 136 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1241), (465), (346), (144), (138), (123), (123), (56), (56), (45), (31), (18), (8), (8), (7), (7), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(959), (480), (213), (43), (13), (9), (9), (8), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(173), (99), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(883), (805), (581), (258), (255), (167), (146), (145), (136), (124), (104), (63), (25), (24), (24), (22), (22), (19), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.
Characteristics The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.
Synonyms: Synonyms: Tumor Necrosis Factor alpha
Purification Affinity Purified
Clone MAb11
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNFa (TNF Antibody Abstract)
Background TNF-alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.
Research Area Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Applications reported: MAb11 has been reported for use in detection of human TNF alpha by ELISA. It can also be used for intracellular flow cytometric analysis.

Applications tested: The biotinylated format for this lot of MAb11 has been tested by sandwich ELISA for the detection of human TNF alpha in combination with MAb1 and can be used at 1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
 image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN281697) Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in th...