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anti-Human Tumor Necrosis Factor antibody for Intracellular Staining

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human
1199
442
329
139
132
118
118
55
55
31
26
18
8
8
7
7
4
4
4
3
2
2
2
1
1
1
Host
Mouse
914
477
201
44
13
9
7
4
3
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
167
95
69
49
32
30
27
26
25
25
25
25
25
25
24
24
24
18
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Intracellular Staining (ICS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
852
796
536
253
251
163
141
140
133
124
101
63
25
24
23
22
18
14
8
6
6
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1003400
$ 385.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696875 ABIN2749005 ICS Neut IHC ELISA Mouse IgG1 Log in to see B-C7
0.0008696875 ABIN808120 ICS IP TCC ELISA Mouse Log in to see NYRhTNFa-E2

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Peptide (BP) 20 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cellular Assay (CA) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 498 Antibodies
ELISA (Capture) 4 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 42 Antibodies
Flow Cytometry (FACS) 437 Antibodies
Functional Studies (Func) 71 Antibodies
Immunoassay (IA) 15 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 95 Antibodies
Immunofluorescence (IF) 132 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1199), (442), (329), (139), (132), (118), (118), (55), (55), (31), (26), (18), (8), (8), (7), (7), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(914), (477), (201), (44), (13), (9), (7), (4), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(167), (95), (69), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (18), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Intracellular Staining (ICS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(852), (796), (536), (253), (251), (163), (141), (140), (133), (124), (101), (63), (25), (24), (23), (22), (18), (14), (8), (6), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Predicted Reactivity Human Tumor Necrosis Factor alpha
Purification Ion exchange chromatography
Immunogen Recombint human TNF-alpha
Clone NYRhTNFa-E2
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name Tumor Necrosis Factor alpha (TNF Antibody Abstract)
Background Human TNF-alpha is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Direct ELISA, Western Blot, Immuneprecipitation, Intracellular staining. In a direct ELISA, a 1:10,000 dilution will yield an O.D. of 0.7 within 10 minutes using alkaline phosphatase-conjugated goat anti-mouse Ig. Optimal dilution has to be determined by user. Antibody might also be suited for other applications not tested so far.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Lyophilized
Buffer NA
Handling Advice Please Note: Always centrifuge product briefly before opening vial. Reconstitute with 0.5 ml sterile H20 or PBS to give a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Protein concentration: 1 mg/ml (after reconstitution).
Storage 4 °C/-20 °C