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anti-Human Tumor Necrosis Factor antibody for Immunofluorescence

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Epitope
AA 100-200, Internal Region
37
30
27
25
23
22
18
15
14
12
10
10
10
7
4
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1199
441
328
139
132
118
118
55
55
31
26
18
8
8
7
7
4
4
4
3
2
2
2
1
1
1
Host
Rabbit
915
476
201
44
13
9
7
4
3
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
167
95
69
49
32
30
27
26
25
25
25
25
25
25
24
24
24
18
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Free Floating) (IHC (ff)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
852
797
536
252
250
162
140
140
134
123
101
63
25
24
23
22
18
14
8
6
6
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN446837
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.27038813 ABIN1533583 IF IHC ELISA Rabbit IgG Log in to see Polyclonal
0.27038813 ABIN2500447 IF IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0011012674 ABIN263069 ICC FACS IF ELISA Biotin Mouse IgG1 kappa Log in to see MAb11 1
0.0011012674 ABIN263070 ICC FACS IF IHC (p) FITC Mouse IgG1 kappa Log in to see MAb11 1
0.0011012674 ABIN498271 IF IHC (p) WB Rabbit Log in to see Polyclonal 2
0.0011012674 ABIN263068 ELISA FACS ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see MAb11 1
0.0011012674 ABIN2659787 BR FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see MAb11 10
0.0011012674 ABIN2506723 ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
0.0011012674 ABIN264364 IF WB Mouse IgG1 Log in to see AS1
0.0011012674 ABIN2659788 BR FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see MAb11 11
0.0011012674 ABIN3187296 ELISA IF IHC Rabbit IgG Internal Region Log in to see Polyclonal
0.0011012674 ABIN116244 EIA Func IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0011012674 ABIN116243 EIA Func IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0011012674 ABIN2377390 FACS IF IP IHC Mouse IgG1 kappa AA 115-130 Log in to see TNF706-P-T2
0.0011012674 ABIN2892043 IF IHC Rabbit Gln178 Log in to see Polyclonal
0.0011012674 ABIN4361130 FACS IF Mouse IgG1 kappa Log in to see J1D9
0.0011012674 ABIN4361132 FACS IF Mouse IgG1 kappa Log in to see J1D9
0.0011012674 ABIN4361131 FACS IF Mouse IgG1 kappa Log in to see J1D9
0.0011012674 ABIN799834 ICC IF IP IHC RIA ELISA WB Rabbit Log in to see Polyclonal
0.0011012674 ABIN2377378 FACS IF IP IHC WB Mouse IgG1 kappa Log in to see MAb1

Top referenced anti-Tumor Necrosis Factor antibody for Immunofluorescence

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 68 Antibodies
ELISpot 2 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 134 Antibodies
Functional Studies (Func) 7 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 66 Antibodies
Immunofluorescence (IF) 97 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Free Floating) (IHC (ff)) 2 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 100-200, Internal Region
(37), (30), (27), (25), (23), (22), (18), (15), (14), (12), (10), (10), (10), (7), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1199), (441), (328), (139), (132), (118), (118), (55), (55), (31), (26), (18), (8), (8), (7), (7), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(915), (476), (201), (44), (13), (9), (7), (4), (3), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(167), (95), (69), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (18), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Free Floating) (IHC (ff)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(852), (797), (536), (252), (250), (162), (140), (140), (134), (123), (101), (63), (25), (24), (23), (22), (18), (14), (8), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN446837) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal portion of the human TNF alpha protein (between residues 100-200) [UniProt P01375]

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN446837) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Gene ID 7124, 21926, 24835
UniProt P01375
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN446837) Images back to top
Application Notes Western Blot 1:250 - 1:500, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:200 - 1:300, Immunohistochemistry-Paraffin 1:200, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry Protocol specific for TNF alpha antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.Immunocytochemistry Protocol specific for TNF alpha antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN446837) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Tumor Necrosis Factor Antibody (ABIN446837)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Tucker, Riggin, Connizzo, Mauck, Steinberg, Kuntz, Soslowsky, Bernstein: "Effect of overuse-induced tendinopathy on tendon healing in a rat supraspinatus repair model." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 34, Issue 1, pp. 161-6, 2016 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Vieira Ramos, Pinheiro, Messa, Delfino, Marqueti, Salvini, Durigan: "Cryotherapy Reduces Inflammatory Response Without Altering Muscle Regeneration Process and Extracellular Matrix Remodeling of Rat Muscle." in: Scientific reports, Vol. 6, pp. 18525, 2016 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)

Xu, Romero, Miller, Kadam, Mial, Plazyo, Garcia-Flores, Hassan, Xu, Tarca, Drewlo, Gomez-Lopez: "An M1-like Macrophage Polarization in Decidual Tissue during Spontaneous Preterm Labor That Is Attenuated by Rosiglitazone Treatment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 6, pp. 2476-91, 2016 (Sample species: Human). Further details: Flow Cytometry,Immunohistochemistry (Frozen Sections)

Szylberg, Janiczek, Popiel, Marszałek: "Expression of COX-2, IL-1β, TNF-α and IL-4 in epithelium of serrated adenoma, adenoma and hyperplastic polyp." in: Archives of medical science : AMS, Vol. 12, Issue 1, pp. 172-8, 2016 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Veruva, Lanman, Isaza, Freeman, Kurtz, Steinbeck: "Periprosthetic UHMWPE Wear Debris Induces Inflammation, Vascularization, and Innervation After Total Disc Replacement in the Lumbar Spine." in: Clinical orthopaedics and related research, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Acosta, Tajiri, Hoover, Kaneko, Borlongan: "Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke." in: Stroke, Vol. 46, Issue 9, pp. 2616-27, 2015 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Free Floating)

Piao, Yuan, Wang, Sun, Liang, Ma: "α-catenin acts as a tumour suppressor in E-cadherin-negative basal-like breast cancer by inhibiting NF-κB signalling." in: Nature cell biology, Vol. 16, Issue 3, pp. 245-54, 2014 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Thomas, Sarver, Yannascoli, Tucker, Kelly, Ahima, Barbe, Soslowsky: "Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 32, Issue 11, pp. 1464-70, 2014 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Castoldi, di Gioia, Bombardi, Maestroni, Carletti, Steckelings, Dahlöf, Unger, Zerbini, Stella: "PREVENTION OF DIABETIC NEPHROPATHY BY COMPOUND 21, SELECTIVE AGONIST OF THE ANGIOTENSIN TYPE 2 RECEPTORS, IN ZUCKER DIABETIC FATTY RATS." in: American journal of physiology. Renal physiology, pp. ajprenal.00247.2014, 2014

Chen, Shi, Zhang, Lei, Long, Su, Pei, Huang: "Scutellarin attenuates hypertension-induced expression of brain Toll-like receptor 4/nuclear factor kappa B." in: Mediators of inflammation, Vol. 2013, pp. 432623, 2013 (Sample species: Rat (Rattus)). Further details: Western Blotting

Kwon, Jung, Hwang, Choi: "Evaluation of Bronchiolar and Alveolar Cell Injuries Induced by Short- and Long-term Exposure to Sidestream Smoke." in: Korean journal of pathology, Vol. 46, Issue 2, pp. 151-61, 2012

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN446837) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 100-200) (ABIN446837) Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Staining of TNF alph...
Immunofluorescence (IF) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 100-200) (ABIN446837) Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody [NBP1-19532] - TNF alpha a...