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anti-Human Tumor Necrosis Factor antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Epitope
AA 180-230
37
28
25
22
18
12
12
10
10
10
7
7
6
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
894
328
141
56
52
31
31
27
24
18
12
12
8
7
5
4
4
4
3
3
2
2
1
1
1
1
Host
Rabbit
672
432
196
43
13
9
9
7
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
158
92
55
36
20
20
15
15
12
11
11
11
11
11
11
11
11
11
11
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
753
704
393
208
138
135
99
98
87
63
56
56
25
25
22
21
18
15
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN677318
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087977585 ABIN722481 IF (p) IHC (p) WB Mouse IgG Log in to see 1F6 1
0.00087977585 ABIN2807094 IF (p) Alexa Fluor 594 Mouse IgG Log in to see 1F6
0.00087977585 ABIN722484 IF (p) Cy3 Mouse IgG Log in to see 1F6
0.00087977585 ABIN722488 IF (p) FITC Mouse IgG Log in to see 1F6
0.00087977585 ABIN722485 IF (p) Cy5 Mouse IgG Log in to see 1F6
0.00087977585 ABIN722486 IF (p) Cy5.5 Mouse IgG Log in to see 1F6
0.00087977585 ABIN722487 IF (p) Cy7 Mouse IgG Log in to see 1F6
0.00087977585 ABIN918031 IF (p) Alexa Fluor 647 Rabbit IgG AA 180-230 Log in to see Polyclonal
0.00087977585 ABIN918042 IF (p) Alexa Fluor 555 Rabbit IgG AA 180-230 Log in to see Polyclonal
0.00087977585 ABIN918032 IF (p) Alexa Fluor 350 Rabbit IgG AA 180-230 Log in to see Polyclonal
0.00087977585 ABIN918034 IF (p) Alexa Fluor 488 Rabbit IgG AA 180-230 Log in to see Polyclonal
0.00087977585 ABIN873382 IF (p) Alexa Fluor 555 Mouse IgG Log in to see 1F6
0.00087977585 ABIN873383 IF (p) Alexa Fluor 647 Mouse IgG Log in to see 1F6
0.00087977585 ABIN873381 IF (p) Alexa Fluor 488 Mouse IgG Log in to see 1F6
0.00087977585 ABIN873380 IF (p) Alexa Fluor 350 Mouse IgG Log in to see 1F6
0.00087977585 ABIN677322 IF (p) Cy5 Rabbit IgG AA 180-230 Log in to see Polyclonal
0.00087977585 ABIN677323 IF (p) Cy5.5 Rabbit IgG AA 180-230 Log in to see Polyclonal
0.00087977585 ABIN677325 IF (p) FITC Rabbit IgG AA 180-230 Log in to see Polyclonal
0.00087977585 ABIN677321 IF (p) Cy3 Rabbit IgG AA 180-230 Log in to see Polyclonal
0.00087977585 ABIN677324 IF (p) Cy7 Rabbit IgG AA 180-230 Log in to see Polyclonal

Top referenced anti-Tumor Necrosis Factor antibody for Immunofluorescence (Paraffin-embedded Sections)

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 57 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 2 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 32 Antibodies
Functional Studies (Func) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 25 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 180-230
(37), (28), (25), (22), (18), (12), (12), (10), (10), (10), (7), (7), (6), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(894), (328), (141), (56), (52), (31), (31), (27), (24), (18), (12), (12), (8), (7), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(672), (432), (196), (43), (13), (9), (9), (7), (4), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(158), (92), (55), (36), (20), (20), (15), (15), (12), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(753), (704), (393), (208), (138), (135), (99), (98), (87), (63), (56), (56), (25), (25), (22), (21), (18), (15), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN677318) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from mouse TNF-alpha
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN677318) Images back to top
Antigen
Alternative Name Tnf alpha (TNF Antibody Abstract)
Background

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Subcellular location: Extracellular


Synonyms: DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tnf
Gene ID 21926
UniProt P06804
Research Area Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN677318) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN677318) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-Tumor Necrosis Factor Antibody (ABIN677318)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Wang, Li, Wei, Xu, Chen, Song, Lu, Wang: "Targeted knockout of TNF‑α by injection of lentivirus‑mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats." in: Molecular medicine reports, Vol. 12, Issue 3, pp. 4389-95, 2015

Sun, Zeng, Cao, Song, Zhang, Liu: "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." in: BioMed research international, Vol. 2015, pp. 919401, 2015

Xu, Zhao, Lin, Wang, Hu, Jiang: "Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus." in: BMC ophthalmology, Vol. 15, pp. 126, 2015

Morishita, Oku, Horie, Tonari, Kida, Okubo, Sugiyama, Takai, Hara, Ikeda: "Systemic simvastatin rescues retinal ganglion cells from optic nerve injury possibly through suppression of astroglial NF-?B activation." in: PLoS ONE, Vol. 9, Issue 1, pp. e84387, 2014

Wang, Hu, Wang, Gu, Gao, Cao, Zheng: "5-Aminolevulinic acid-mediated sonodynamic therapy reverses macrophage and dendritic cell passivity in murine melanoma xenografts." in: Ultrasound in medicine & biology, Vol. 40, Issue 9, pp. 2125-33, 2014

Wang, Zhang, Gu, Ding, Fan, Zhu, Shi, Hong, Kong: "Morin reduces hepatic inflammation-associated lipid accumulation in high fructose-fed rats via inhibiting sphingosine kinase 1/sphingosine 1-phosphate signaling pathway." in: Biochemical pharmacology, Vol. 86, Issue 12, pp. 1791-804, 2013

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN677318) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 180-230) (ABIN677318) Antigen: 0.2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 180-230) (ABIN677318) Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha P...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 180-230) (ABIN677318) Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alp...
'Independent Validation' Badge
Lot Number 140113
Method validated Immunofluorescence
Positive Control Normal mouse lung tissue
Negative Control Normal mouse adipose tissue
Notes Fluorescent signal is detected in positive control, but not in negative control or isotype controls.
Primary Antibody
  • Antibody: Tumor Necrosis Factor (TNF)
  • Catalog number: ABIN677318
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 140113
Isotype Control Antibody
Secondary Antibody
  • Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)
  • Catalog number: Log in to see
  • Supplier: Log in to see
  • Lot number: Not available
Controls
  • Positive control: FFPE normal mouse lung tissue
  • Negative Control: FFPE normal mouse fat tissue
  • Isotype antibody control: tissue sections treated with Rabbit IgG Isotype at 1ug/ul
  • Secondary antibody only control: positive and negative control tissue sections treated with Goat anti-Rabbit Alexa 647 secondary antibody only. Any staining observed is due to non-specific binding of secondary antibody.
Protocol
  • Paraffin embedded positive and negative control tissue sections were deparaffinized and underwent antigen retrieval using 10 mM sodium citrate pH 6.0 in a pressure cooker for 10 min.
  • The tissue sections were incubated with 1 mg/mL sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
  • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
  • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen).
  • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
Experimental Notes Nothing to note.
Validation Images
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 1: confocal image of positive control (mouse lung) with anti-TNF alpha antibody
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 2: confocal image of negative control (mouse adipose) with anti-TNF alpha antib...
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 3: confocal image of positive control (mouse lung) with isotype control
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 4: confocal image of negative control (mouse adipose) with isotype control
Immunofluorescence validation image for anti-Tumor Necrosis Factor (TNF) (AA 180-230) antibody (ABIN677318) Panel 5: confocal image of positive control (mouse lung) with secondary antibody only