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anti-Human Tumor Necrosis Factor antibody for Immunohistochemistry (Frozen Sections)

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human
1232
462
344
144
138
123
123
56
52
45
31
18
8
7
5
4
4
4
3
3
3
2
2
1
1
1
Host
Rabbit
953
478
210
43
13
9
9
7
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
98
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
879
797
577
251
249
162
145
142
135
121
102
62
25
25
23
22
22
17
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN116868
$ 1,435.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088411616 ABIN116867 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal 2
0.00088411616 ABIN967215 IHC (fro) FACS Mouse IgG1 kappa AA 115-130 Log in to see B428 1
0.00088411616 ABIN1109383 Func EIA IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 52B83 4
0.00088411616 ABIN1109384 Func EIA IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 4H31 1
0.00088411616 ABIN1109408 IHC (fro) IHC (p) WB FITC Mouse IgG1 Log in to see 52B83 5
0.00088411616 ABIN305730 FACS IHC (fro) IHC (p) ELISA WB Mouse IgG1 Log in to see 52B83
0.00088411616 ABIN126020 EIA FACS IHC (fro) Rat IgG1 Log in to see MP9-20A4 2
0.00088411616 ABIN1109397 IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 52B83 5
0.00088411616 ABIN126018 EIA Func FACS IHC (fro) Rat IgG1 Log in to see MP9-20A4 2
0.00088411616 ABIN600294 IHC (fro) FACS IHC (p) ELISA WB Mouse IgG1 Log in to see 52B83
0.00088411616 ABIN1109388 Func EIA IHC (fro) IP WB Mouse IgG1 Log in to see 4H31 2
0.00088411616 ABIN225261 FACS IHC (fro) Mouse IgG1 Log in to see MAb11
0.00088411616 ABIN1690740 Func ICC IHC (fro) FACS ELISA PE Mouse IgG1 Log in to see MAb11 5
0.00088411616 ABIN959542 IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see 52B83
0.00088411616 ABIN3201314 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 77-233 Log in to see
0.00088411616 ABIN263068 ICC FACS IHC (fro) IF IHC (p) IHC Mouse IgG1 kappa Log in to see MAb11
0.00088411616 ABIN1502427 IHC (fro) Mouse kappa, IgG1 Log in to see B428
0.00088411616 ABIN2191874 IHC (fro) FACS IHC (p) WB FITC Mouse IgG1 Log in to see 52B83 4
0.00088411616 ABIN268274 IA IHC (fro) IHC (p) IHC Mouse IgG1 Log in to see 2C8
0.00088411616 ABIN610506 IHC (fro) IHC (p) ELISA Mouse IgG1, kappa Log in to see 4C6-H8

Top referenced anti-Tumor Necrosis Factor antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cellular Assay (CA) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 500 Antibodies
ELISA (Capture) 6 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Enzyme Immunoassay (EIA) 42 Antibodies
Flow Cytometry (FACS) 473 Antibodies
Functional Studies (Func) 72 Antibodies
Immunoassay (IA) 16 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 96 Antibodies
Immunofluorescence (IF) 136 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1232), (462), (344), (144), (138), (123), (123), (56), (52), (45), (31), (18), (8), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(953), (478), (210), (43), (13), (9), (9), (7), (4), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (98), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(879), (797), (577), (251), (249), (162), (145), (142), (135), (121), (102), (62), (25), (25), (23), (22), (22), (17), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN116868) Images
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Protein G Chromatography.
Immunogen Repeated immunizations with Recombinant Human TNF alpha produced in E.coli.

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN116868) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 9606
UniProt P01375
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN116868) Images back to top
Application Notes This IgG fraction of anti-Human TNFa has been tested for use in Neutralizations, ELISA(1/1,000-1/5,000), Immunohistochemistry, Radioimmunoassay, Immunoprecipitation,Immunohistochemistry and Immunoblotting (1/500-1/2,000). It recognizes the 17,000 MWTNFa . Reactivity in other immunoassays is unknown. Recommended Dilutions: Immunoblot using HRP Goat-anti-Rabbit IgG [H&L] and TMB as a substrate. A workingdilution range of 1/100-1/200 is suggested for this application to detect TNFa fromsupernatants or lysates of 2 x 10^6 endotoxin-stimulated human peripheral bloodmononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serumplus 10 ng/mL E. coli LPS.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN116868) Images back to top
Concentration 5.0 mg/mL (by UV Absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 without stabilizers or preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Tumor Necrosis Factor Antibody (ABIN116868)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Birkas Kovats, Palik, Faludi, Cseh: "[Possible connection between ghrelin, resistin and TNF-alpha levels and the metabolic syndrome caused by atypical antipsychotics]" in: Neuropsychopharmacologia Hungarica : a Magyar Pszichofarmakológiai Egyesület lapja = official journal of the Hungarian Association of Psychopharmacology, Vol. 7, Issue 3, pp. 132-9, 2006

Cseh, Beutler: "Alternative cleavage of the cachectin/tumor necrosis factor propeptide results in a larger, inactive form of secreted protein." in: The Journal of biological chemistry, Vol. 264, Issue 27, pp. 16256-60, 1989

Nedwin, Naylor, Sakaguchi, Smith, Jarrett-Nedwin, Pennica, Goeddel, Gray: "Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization." in: Nucleic acids research, Vol. 13, Issue 17, pp. 6361-73, 1985