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anti-Human Tumor Necrosis Factor antibody for Western Blotting

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1199
441
328
139
132
118
118
55
55
31
26
18
8
8
7
7
4
4
4
3
2
2
2
1
1
1
Host
Rabbit
915
476
201
44
13
9
7
4
3
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
167
95
69
49
32
30
27
26
25
25
25
25
25
25
24
24
24
18
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
852
797
536
252
251
163
140
140
134
124
101
63
25
24
23
22
18
14
8
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN498271
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12498369 ABIN1582064 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 2
0.12498369 ABIN1724680 ELISA WB Mouse IgG1 Log in to see 2A9B9 3
0.12498369 ABIN1109394 WB Mouse IgG1 Log in to see 2A9B9 3
0.12498369 ABIN782382 EIA IHC (p) WB Mouse IgG1 AA 1-234 Log in to see M1-C4
0.12498369 ABIN153474 IHC (p) IHC ELISA WB Rabbit Log in to see Polyclonal 7
0.12498369 ABIN1997173 ELISA WB Rabbit IgG AA 77-233 Log in to see Polyclonal 1
0.12498369 ABIN2779181 WB Mouse IgG Log in to see 1
0.12498369 ABIN2779182 WB Mouse IgG Log in to see 1
0.12498369 ABIN657455 FACS WB Rabbit Ig AA 187-216, C-Term Log in to see Polyclonal
0.12498369 ABIN452504 EIA WB Mouse IgG1 Log in to see B1E4
0.12498369 ABIN452506 EIA WB Mouse IgG1 Log in to see C5G7
0.12498369 ABIN452505 EIA WB Mouse IgG1 Log in to see C2A7
0.12498369 ABIN452501 EIA WB Mouse IgG2b Log in to see C2H9
0.12498369 ABIN452507 EIA WB Mouse IgG1 Log in to see C4C7
0.12498369 ABIN563205 IHC (p) ELISA WB Mouse IgG1 kappa AA 1-233, full length Log in to see M1-C4 1
0.12498369 ABIN2707185 IHC WB Rabbit Center Log in to see Polyclonal
0.12498369 ABIN222906 IHC Neut WB Rabbit IgG Log in to see Polyclonal
0.12498369 ABIN1875124 IP WB Rabbit IgG Log in to see Polyclonal
0.12498369 ABIN2500451 ELISA WB Mouse IgG1 Log in to see 2A9B9
0.12498369 ABIN3020863 IHC WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-Tumor Necrosis Factor antibody for Western Blotting

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 68 Antibodies
ELISpot 2 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 134 Antibodies
Functional Studies (Func) 7 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 66 Antibodies
Immunofluorescence (IF) 97 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Free Floating) (IHC (ff)) 2 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1199), (441), (328), (139), (132), (118), (118), (55), (55), (31), (26), (18), (8), (8), (7), (7), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(915), (476), (201), (44), (13), (9), (7), (4), (3), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(167), (95), (69), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (18), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(852), (797), (536), (252), (251), (163), (140), (140), (134), (124), (101), (63), (25), (24), (23), (22), (18), (14), (8), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN498271) Images
Specificity This antibody detects endogenous levels of TNFα protein. (region surrounding Gln178)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity Chromatography using epitope-specific immunogen

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN498271) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Molecular Weight approx. 17, 25 kDa
Gene ID 7124
NCBI Accession NP_000585
UniProt P01375
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN498271) Images back to top
Application Notes Immunoflourescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN498271) Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Tumor Necrosis Factor Antibody (ABIN498271)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Yu, Wang, Du, Ma, Chen, You, Liu, Ling, Yang, Ji: "Akebia Saponin D attenuates amyloid ?-induced cognitive deficits and inflammatory response in rats: involvement of Akt/NF-?B pathway." in: Behavioural brain research, Vol. 235, Issue 2, pp. 200-9, 2012

Chae, Park, Hwang, Kwon, Lim, Yi, Han, Kim, Baek: "The effect of antioxidants on the production of pro-inflammatory cytokines and orthodontic tooth movement." in: Molecules and cells, Vol. 32, Issue 2, pp. 189-96, 2011

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN498271) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN498271) Immunohistochemistry analysis of TNF-alpha Antibody in paraffin-embedded human...
Western Blotting (WB) image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN498271) Western Blot analysis of TNF-alpha Antibody at 1/500 dilution.