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anti-Monkey Tumor Necrosis Factor antibody for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Human, Monkey
1228
460
359
142
135
119
119
60
56
40
28
19
11
10
9
7
4
4
4
4
4
2
1
1
1
Host
Mouse
929
490
201
44
13
9
7
6
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to FITC
169
97
69
49
32
30
27
26
25
25
25
25
25
25
24
24
24
18
13
9
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS)
906
866
599
308
280
229
202
180
135
122
97
63
27
26
25
22
20
18
14
8
8
7
6
6
5
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN264363
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN305730 FACS IHC (fro) IHC (p) ELISA WB Mouse IgG1 Log in to see 52B83
1 ABIN1109400 Func EIA FACS WB Rabbit IgG Log in to see Polyclonal
1 ABIN3200991 ELISA FACS Func ICC IHC (fro) APC Mouse IgG1 Log in to see MAb11
1 ABIN3200802 ELISA FACS Func ICC IHC (fro) FITC Mouse IgG1 Log in to see MAb11
1 ABIN3200803 ELISA FACS Func ICC IHC (fro) PE Mouse IgG1 Log in to see MAb11
1 ABIN225261 FACS IHC (fro) Mouse IgG1 Log in to see MAb11
1 ABIN600294 IHC (fro) FACS IHC (p) ELISA WB Mouse IgG1 Log in to see 52B83
1 ABIN3200801 ELISA FACS Func ICC IHC (fro) Mouse IgG1 Log in to see MAb11
1 ABIN959542 IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see 52B83
1 ABIN199738 FACS Neut ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN598267 FACS IP WB Mouse IgG1 Log in to see
1 ABIN3040095 ELISA FACS IF/ICC IHC Mouse IgG1 Log in to see
1 ABIN3040097 ELISA FACS IF/ICC IHC APC Mouse IgG1 Log in to see
1 ABIN3040098 ELISA FACS IF/ICC IHC FITC Mouse IgG1 Log in to see
1 ABIN3040099 ELISA FACS IF/ICC IHC PE Mouse IgG1 Log in to see

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human Monkey
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies 6 Antibodies
Activity Inhibition (Act Inh) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 27 Antibodies
Immunohistochemistry (Free Floating) (IHC (ff)) 2 Antibodies
Western Blotting (WB) 540 Antibodies 26 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Radioimmunoassay (RIA) 17 Antibodies
Protein Assay (PrA) 2 Antibodies
Neutralization (Neut) 62 Antibodies 2 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Inhibition Assay (InhA) 2 Antibodies
Immunostaining (ISt) 3 Antibodies
Immunoprecipitation (IP) 95 Antibodies 6 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 284 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human, Monkey
(1228), (460), (359), (142), (135), (119), (119), (60), (56), (40), (28), (19), (11), (10), (9), (7), (4), (4), (4), (4), (4), (2), (1), (1), (1)
Host Mouse
(929), (490), (201), (44), (13), (9), (7), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to FITC
(169), (97), (69), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (18), (13), (9), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(906), (866), (599), (308), (280), (229), (202), (180), (135), (122), (97), (63), (27), (26), (25), (22), (20), (18), (14), (8), (8), (7), (6), (6), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Characteristics Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin, Tumor necrosis factor ligand superfamily member 2
Purification Protein G Chromatography
Purity > 95 % by SDS-PAGE
Immunogen Genename: TNF
Clone AS1
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
UniProt P01375
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol PBMC: Add 10 ml of MAB/10e6 PBMC in 100 ml PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Buffer PBS containing 0.05 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months