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|Antigen||Tumor Necrosis Factor (TNF) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This Tumor Necrosis Factor antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
|5 references available|
|Supplier||Log in to see|
Product Details anti-Tumor Necrosis Factor AntibodyTarget Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN119359) Images
|Purification||Affinity chromatography on Protein G|
|Immunogen||Recombinant murine TNF alpha expressed in E. coli.|
Target Details Tumor Necrosis FactorProduct Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN119359) Images back to top
|Alternative Name||Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)|
|Background||TNA alpha is a 17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immuno-regulatory role.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2|
|Research Area||Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus|
Application DetailsProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN119359) Images back to top
Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. ELISA: 1 μg/mL - 10 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN119359) Images back to top
|Buffer||PBS, pH 7.4, 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-Tumor Necrosis Factor antibody (ABIN119359)Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Hostager, Bishop: "Role of TNF receptor-associated factor 2 in the activation of IgM secretion by CD40 and CD120b." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 7, pp. 3318-22, 2002
Scanga, Mohan, Joseph, Yu, Chan, Flynn: "Reactivation of latent tuberculosis: variations on the Cornell murine model." in: Infection and immunity, Vol. 67, Issue 9, pp. 4531-8, 1999
Ross, Williams, Mason, Mauri, Marinova-Mutafchieva, Malfait, Maini, Feldmann: "Suppression of TNF-alpha expression, inhibition of Th1 activity, and amelioration of collagen-induced arthritis by rolipram." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 12, pp. 6253-9, 1998
Yang, Kawamura, Zhu, Mitsuyama: "Involvement of natural killer cells in nitric oxide production by spleen cells after stimulation with Mycobacterium bovis BCG. Study of the mechanism of the different abilities of viable and killed BCG." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 12, pp. 5728-35, 1996
Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992