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anti-Mouse (Murine) Tumor Necrosis Factor antibody for ELISA

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Mouse (Murine)
1233
462
344
144
138
123
123
56
52
45
31
18
8
7
5
4
4
4
3
3
3
2
2
1
1
1
Host
Rat
953
479
210
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
98
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
ELISA
880
797
577
252
249
163
145
142
135
121
103
63
25
25
23
22
22
17
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN263141
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32666028 ABIN2476888 Func FACS ELISA WB Rat IgG1 Log in to see MP6-XT3 4
0.32666028 ABIN2497021 ELISA PE Rat IgG1 Log in to see
0.32666028 ABIN2497020 ELISA FITC Rat IgG1 Log in to see
0.32666028 ABIN2497027 FACS ELISA Biotin Rat IgG1 Log in to see
0.32666028 ABIN2497045 IHC (af) FACS ELISA Rat IgG1 Log in to see
0.32666028 ABIN2497046 IHC (af) FACS ELISA Rat IgG1 Log in to see
0.0012121415 ABIN1582064 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 2
0.0012121415 ABIN967462 Inhibition ELISA WB Rat IgG1 Log in to see MP6-XT22 5
0.0012121415 ABIN1533583 IF IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012121415 ABIN1582139 IP IHC ELISA WB Mouse IgG Log in to see 2
0.0012121415 ABIN1177009 Neut ELISA WB Rat IgG1, kappa Log in to see MP6-XT3 2
0.0012121415 ABIN1177297 Neut ELISA Rat IgG1 Log in to see G281-2626 3
0.0012121415 ABIN967569 IP Neut ELISA WB Armenian Hamster IgG1, lambda Log in to see TN3-19-12 6
0.0012121415 ABIN217631 FACS IHC (fro) ELISA Rat IgG1 Log in to see
0.0012121415 ABIN2000813 ELISA Rabbit IgG AA 80-235 Log in to see 9 1
0.0012121415 ABIN2689929 ELISA WB Biotin Rat IgG1, kappa Log in to see MP6 2
0.0012121415 ABIN2689928 ELISA Biotin Human Ig Fraction, kappa Log in to see 516D1A1 1
0.0012121415 ABIN2000817 ELISA Rabbit IgG AA 80-235 Log in to see Polyclonal 1
0.0012121415 ABIN2000815 ELISA Rabbit IgG AA 80-235 Log in to see Polyclonal 1
0.0012121415 ABIN289560 Inhibition ELISA WB Rabbit Log in to see Polyclonal 1

Top referenced anti-Tumor Necrosis Factor antibody for ELISA

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 7 Antibodies
Blocking Reagent (BR) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 156 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 4 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 152 Antibodies
Functional Studies (Func) 19 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 69 Antibodies
Immunofluorescence (IF) 85 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 69 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine)
(1233), (462), (344), (144), (138), (123), (123), (56), (52), (45), (31), (18), (8), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rat
(953), (479), (210), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (98), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application ELISA
(880), (797), (577), (252), (249), (163), (145), (142), (135), (121), (103), (63), (25), (25), (23), (22), (22), (17), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity TNF- alpha (6B8)
Purification affinity purified
Immunogen recombinant mouse TNF- alpha
Clone 6B8
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background TNF- alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principallyCD4+), and NK-cells. Many transformed cell lines also secrete TNF- alpha. Monomericmouse TNF- alpha is 156 amino acid protein (N-glycosylated) with a reported molecularweight of 17.5 kD protein. TNF- alpha forms multimeric complexes, stable trimers aremost common in solution. A 26 kD membrane form of TNF- alpha has also beendescribed. TNF- alpha binding to surface receptors elicits a wide array of biologicactivities including: cytolysis and cytostasis of many tumor cell lines in vitro,hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhancedchemotaxis and phagocytosis in neutrophils. The 6B8 antibody reacts with mouse andrat tumor necrosis factor- alpha (TNF- alpha). Alternate Names: anti-Tumor necrosis factor-alpha antibody, anti-Cachectin antibody, anti-Necrosinantibody, anti-Macrophage cytotoxic factor (MCF) antibody, anti-Differentiation inducingfactor (DIF) antibody, anti-TNFSF-2 antibody, anti-TNF-a antibody, anti-TNF-alphaantibody, anti-TNF alpha antibody.
Gene Symbol: TNF
Gene ID 7124
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 mug/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF- alpha recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Recommended dilutions: ELISA 1:100-1:2000
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS [pH 7.2], Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
ELISA image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN263141) anti-Tumor Necrosis Factor (TNF) antibody