You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) Tumor Necrosis Factor antibody for Intracellular Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Mouse (Murine), Rat (Rattus)
895
328
141
56
52
31
31
27
24
18
12
12
8
7
5
4
4
4
3
3
2
2
1
1
1
1
Host
Hamster
672
433
196
43
13
9
8
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
158
92
55
36
20
20
15
15
12
11
11
11
11
11
11
11
11
11
11
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
753
705
392
208
138
136
99
98
87
63
56
56
25
25
22
22
18
15
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2665424
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008693091 ABIN2665423 FACS ICFC Hamster IgG Log in to see TN3-19-12 9

Top referenced anti-Tumor Necrosis Factor antibody for Intracellular Flow Cytometry

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 57 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 2 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 32 Antibodies
Functional Studies (Func) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 25 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(895), (328), (141), (56), (52), (31), (31), (27), (24), (18), (12), (12), (8), (7), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Host Hamster
(672), (433), (196), (43), (13), (9), (8), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(158), (92), (55), (36), (20), (20), (15), (15), (12), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
(753), (705), (392), (208), (138), (136), (99), (98), (87), (63), (56), (56), (25), (25), (22), (22), (18), (15), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
Supplier Log in to see

Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2665424) Images
Cross-Reactivity Rabbit
Purification The antibody was purified by affinity chromatography.
Clone TN3-19-12
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2665424) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes, stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The TN3-19.12 antibody reacts with mouse, rat, and rabbit tumor-necrosis factor-α (TNF-α) proteins. The TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-α.
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2665424) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN2665424) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-Tumor Necrosis Factor Antibody (ABIN2665424)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Simkins, Hyde, Farrand, Ong, Degli-Esposti, Hermans, Ronchese: "Administration of alpha-galactosylceramide impairs the survival of dendritic cell subpopulations in vivo." in: Journal of leukocyte biology, Vol. 89, Issue 5, pp. 753-62, 2011

Takahashi, Kapás, Fang, Krueger: "An anti-tumor necrosis factor antibody suppresses sleep in rats and rabbits." in: Brain research, Vol. 690, Issue 2, pp. 241-4, 1996

Rabinovici, Bugelski, Esser, Hillegass, Griswold, Vernick, Feuerstein: "Tumor necrosis factor-alpha mediates endotoxin-induced lung injury in platelet activating factor-primed rats." in: The Journal of pharmacology and experimental therapeutics, Vol. 267, Issue 3, pp. 1550-7, 1994

Tracey, Cerami: "Tumor necrosis factor: an updated review of its biology." in: Critical care medicine, Vol. 21, Issue 10 Suppl, pp. S415-22, 1993

Molloy, ORiordain, Holzheimer, Nestor, Collins, Mannick, Rodrick: "Mechanism of increased tumor necrosis factor production after thermal injury. Altered sensitivity to PGE2 and immunomodulation with indomethacin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 4, pp. 2142-9, 1993

Merrick, He, Craig, Clark, Corsini, Rosenthal, Mansfield, Selkirk: "Two dimensional gel electrophoresis of cellular and secreted proteins from rat alveolar macrophages after lipopolysaccharide treatment." in: Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society, Vol. 2, Issue 6, pp. 177-87, 1992

Beutler, Cerami: "The biology of cachectin/TNF--a primary mediator of the host response." in: Annual review of immunology, Vol. 7, pp. 625-55, 1989

Sheehan, Ruddle, Schreiber: "Generation and characterization of hamster monoclonal antibodies that neutralize murine tumor necrosis factors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 11, pp. 3884-93, 1989

Beutler, Cerami: "Tumor necrosis, cachexia, shock, and inflammation: a common mediator." in: Annual review of biochemistry, Vol. 57, pp. 505-18, 1988

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2665424) back to top
Supplier Images
ELISA image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN2665424) anti-Tumor Necrosis Factor (TNF) antibody
ELISA image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN2665424) anti-Tumor Necrosis Factor (TNF) antibody (Image 2)