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anti-Mouse (Murine) Tumor Necrosis Factor antibody for Neutralization

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Mouse (Murine)
895
328
142
56
52
31
31
27
24
18
12
12
8
7
5
4
4
4
3
3
2
2
1
1
1
1
Host
Rat
672
433
195
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
158
92
55
36
20
20
15
15
12
11
11
11
11
11
11
11
11
11
11
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Neutralization (Neut), ELISA, Western Blotting (WB)
752
704
393
208
137
136
99
98
87
63
56
56
25
25
22
22
18
15
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1177009
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00086914125 ABIN1177297 Neut ELISA Rat IgG1 Log in to see G281-2626 3
0.00086914125 ABIN967569 IP Neut ELISA WB Armenian Hamster IgG1, lambda Log in to see TN3-19-12 6
0.00086914125 ABIN107955 IP IHC Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
0.00086914125 ABIN226594 IHC (fro) Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.00086914125 ABIN265631 BR IP Neut ELISA WB Armenian Hamster IgG Log in to see TN3-19-12
0.00086914125 ABIN199737 FACS IHC (fro) IHC (p) Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.00086914125 ABIN2451833 IP IHC (p) Neut ELISA WB Rabbit IgG AA 80-235 Log in to see Polyclonal
0.00086914125 ABIN2689934 Neut ELISA WB Rat IgG1 Log in to see G281 2
0.00086914125 ABIN2689936 Inhibition IP Neut ELISA WB Armenian Hamster IgG1, lambda Log in to see TN3 6
0.00086914125 ABIN465869 IHC Neut ELISA WB Rabbit Log in to see Polyclonal
0.00086914125 ABIN465805 IHC Neut ELISA WB Goat Log in to see Polyclonal
0.00086914125 ABIN3042817 IP Neut IHC (p) ELISA WB Rabbit IgG AA 80-235 Log in to see Polyclonal
0.00086914125 ABIN335672 Neut Rat IgG1 kappa Log in to see MP6-XT22
0.00086914125 ABIN334146 Neut WB Rat IgG1 Log in to see
0.00086914125 ABIN283063 FACS ICS Neut ELISA PE-Cy7 Armenian Hamster IgG Log in to see TN3-19-12
0.00086914125 ABIN107954 IP IHC Neut RIA ELISA WB Rabbit Log in to see Polyclonal
0.00086914125 ABIN2377361 IP Neut IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.00086914125 ABIN603915 FACS Neut Rat IgG2a Log in to see
0.00086914125 ABIN470638 ICC IHC (fro) IP Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
0.00086914125 ABIN959221 Neut Rat IgG1, kappa Log in to see MP6-XT3

Top referenced anti-Tumor Necrosis Factor antibody for Neutralization

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 7 Antibodies
Blocking Reagent (BR) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 143 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 4 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 109 Antibodies
Functional Studies (Func) 19 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 25 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 48 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine)
(895), (328), (142), (56), (52), (31), (31), (27), (24), (18), (12), (12), (8), (7), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rat
(672), (433), (195), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(158), (92), (55), (36), (20), (20), (15), (15), (12), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Neutralization (Neut), ELISA, Western Blotting (WB)
(752), (704), (393), (208), (137), (136), (99), (98), (87), (63), (56), (56), (25), (25), (22), (22), (18), (15), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1177009) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse TNF. Neutralization activity may be measured using a cytolysis assay for MTT dye conversion with 70 pg/mL recombinant mouse TNF (Cat. No. 554589) and L929 at 2x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse TNF for 60 minutes).
50% Neutralization (ND50) at 30 - 300 ng/mL
>95% Neutralization at 2 - 35 myg/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse TNF Recombinant Protein
Clone MP6-XT3
Isotype IgG1, kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1177009) Images back to top
Antigen
Alternative Name TNF (TNF Antibody Abstract)
Background The MP6-XT3 antibody reacts with mouse TNF (TNF-alpha). Cross reactivity with lymphotoxin-alpha (LT-alpha , TNF-beta) has not been determined.
Synonyms: Tumor Necrosis Factor
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1177009) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN1177009) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-Tumor Necrosis Factor Antibody (ABIN1177009)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, 2008

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992