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anti-Pig (Porcine) Tumor Necrosis Factor antibody for Enzyme Immunoassay

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Pig (Porcine)
1242
465
346
144
138
123
123
56
55
45
31
18
8
8
7
7
4
4
4
3
3
2
2
1
1
1
Host
Mouse
959
480
213
43
13
9
9
8
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
173
99
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
882
805
582
258
255
167
146
145
136
124
103
62
25
25
24
22
22
19
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN119944
$ 643.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN119943 EIA Func Mouse IgG1 Log in to see 4F4

Top referenced anti-Tumor Necrosis Factor antibody for Enzyme Immunoassay

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Pig (Porcine)
ELISA 43 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 15 Antibodies
Functional Studies (Func) 21 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 28 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Pig (Porcine)
(1242), (465), (346), (144), (138), (123), (123), (56), (55), (45), (31), (18), (8), (8), (7), (7), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(959), (480), (213), (43), (13), (9), (9), (8), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(173), (99), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
(882), (805), (582), (258), (255), (167), (146), (145), (136), (124), (103), (62), (25), (25), (24), (22), (22), (19), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN119944) Images
Specificity This antibody is specific for tumour necrosis factor- alpha (TNF alpha). The antibody recognises both native and recombinant TNF alpha. Clone 6E10 is reported to neutralise the effects of porcine TNF alpha in in vitro cytotoxicity assays using WEHI 164 clone 13 murine fibrosarcoma cells.
Purification Affinity Chromatography on Protein A
Immunogen Recombinant porcine TNF alpha.
Clone 6-00E-010
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN119944) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background TNF alpha is expressed by a range of cells including macrophages and monocytes, and is an important mediator of the porcine inflammatory response.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 397086
NCBI Accession NP_999187
UniProt P23563
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN119944) Images back to top
Application Notes ELISA. Western Blot. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.015 EU/ug
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN119944) Images back to top
Concentration 0.5 mg/mL
Buffer PBS without preservatives
Preservative Without preservative
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody at 2 - 8 °C. DO NOT FREEZE!

References for anti-Tumor Necrosis Factor Antibody (ABIN119944)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Zelnickova, Faldyna, Stepanova, Ondracek, Kovaru: "Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining." in: Journal of immunological methods, Vol. 327, Issue 1-2, pp. 18-29, 2007

Su, Prestwood, Otto, Prickett, McGraw: "Monoclonal antibodies to porcine tumor necrosis factor alpha: development of an enzyme-linked immunosorbent assay." in: Journal of immunoassay, Vol. 13, Issue 2, pp. 253-72, 1992