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anti-Pig (Porcine) Tumor Necrosis Factor antibody for ELISA

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human, Monkey, Pig (Porcine), Baboon
1233
462
344
144
138
123
123
56
52
45
31
18
8
7
5
4
4
4
3
3
3
2
2
1
1
1
Host
Mouse
953
479
210
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Biotin
172
98
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA
880
797
577
252
249
163
145
142
135
121
103
63
25
25
23
22
22
17
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN263069
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012121415 ABIN1582064 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 2
0.0012121415 ABIN1582141 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal 5
0.0012121415 ABIN964798 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012121415 ABIN964799 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012121415 ABIN1582139 IP IHC ELISA WB Mouse IgG Log in to see 2
0.0012121415 ABIN1104722 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012121415 ABIN1690740 Func ICC IHC (fro) FACS ELISA PE Mouse IgG1 Log in to see MAb11 5
0.0012121415 ABIN1981885 Func IHC (f) ICC FACS ELISA FITC Mouse IgG1 Log in to see MAb11 5
0.0012121415 ABIN2749097 Func IHC (f) ICC FACS ELISA Mouse IgG1 Log in to see MAb11 4
0.0012121415 ABIN2749165 Func IHC (f) ICC FACS ELISA APC Mouse IgG1 Log in to see MAb11 4
0.0012121415 ABIN3201320 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 77-232 Log in to see
0.0012121415 ABIN1078626 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 77-232 Log in to see Polyclonal
0.0012121415 ABIN2476866 Func ELISA Mouse IgG1 Log in to see 4F4 1
0.0012121415 ABIN2476867 Func ELISA WB Mouse IgG1 Log in to see 6E10 3
0.0012121415 ABIN2629354 ELISA Biotin Rabbit IgG AA 77-232 Log in to see Polyclonal
0.0012121415 ABIN2629368 ELISA FITC Rabbit IgG AA 77-232 Log in to see Polyclonal
0.0012121415 ABIN254428 Func ELISA WB Mouse IgG1 Log in to see 6-E10
0.0012121415 ABIN2574880 ELISA Rabbit IgG AA 77-232 Log in to see Polyclonal
0.0012121415 ABIN2574894 ELISA Mouse IgG AA 77-232 Log in to see
0.0012121415 ABIN2629360 ELISA WB Biotin Mouse IgG AA 77-232 Log in to see

Top referenced anti-Tumor Necrosis Factor antibody for ELISA

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Pig (Porcine) Monkey
ELISA 42 Antibodies 16 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 8 Antibodies
Flow Cytometry (FACS) 15 Antibodies 13 Antibodies
Functional Studies (Func) 21 Antibodies 14 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 28 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human, Monkey, Pig (Porcine), Baboon
(1233), (462), (344), (144), (138), (123), (123), (56), (52), (45), (31), (18), (8), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(953), (479), (210), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to Biotin
(172), (98), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA
(880), (797), (577), (252), (249), (163), (145), (142), (135), (121), (103), (63), (25), (25), (23), (22), (22), (17), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263069) Images
Specificity TNF- alpha (MAb11)
Cross-Reactivity (Details) Predicted cross-reactivity based on sequence identity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Sooty Mangabey, Swine (Pig, Porcine).
Purification Affinity purified
Immunogen E. coli-expressed, recombinant human TNF- alpha
Clone MAb11
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263069) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background TNF- alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principallyCD4+), and NK-cells. Many transformed cell lines also secrete TNF- alpha. Monomerichuman TNF- alpha is a 157 amino acid protein (non-glycosylated) with a reportedmolecular weight of 17 kD. TNF- alpha forms multimeric complexes, stable trimers aremost common in solution. A 26 kD membrane form of TNF- alpha has also beendescribed. TNF- alpha binding to surface receptors elicits a wide array of biologicactivities including: cytolysis and cytostasis of many tumor cell lines in vitro,hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhancedchemotaxis and phagocytosis in neutrophils. The MAb11 antibody can neutralize thebioactivity of natural or recombinant TNF- alpha. Alternate Names: anti-Tumor necrosis factor-alpha antibody, anti-Cachectin antibody, anti-Necrosinantibody, anti-Macrophage cytotoxic factor (MCF) antibody, anti-Differentiation inducingfactor (DIF) antibody, anti-TNFSF2 antibody, anti-TNF-a antibody, anti-TNF-alphaantibody.
Gene Symbol: TNF
Gene ID 7124
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263069) Images back to top
Application Notes The purified MAb11 has been tested by blocking fluorochrome conjugated MAb11 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody ( le 5.0 µg/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application.
Recommended dilutions: ELISA 1:100-1:2000, Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN263069) Images back to top
Concentration 0.5 mg/mL
Buffer PBS [pH 7.2], Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C

References for anti-Tumor Necrosis Factor Antibody (ABIN263069)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Baker, Murphy, Lopez, Garcia: "Conversion of a capture ELISA to a Luminex xMAP assay using a multiplex antibody screening method." in: Journal of visualized experiments : JoVE, Issue 65, 2012 (Sample species: Human). Further details: ELISA

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263069) back to top
Supplier Images
ELISA image for anti-Tumor Necrosis Factor antibody (TNF)  (Biotin) (ABIN263069) anti-Tumor Necrosis Factor (TNF) antibody (Biotin)