anti-Pig (Porcine) Tumor Necrosis Factor antibody for Functional Studies

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Reactivity
Pig (Porcine)
1253
462
357
144
136
119
118
61
54
41
29
17
14
12
9
7
6
4
4
4
3
2
1
1
1
1
Host
Mouse
936
520
184
46
10
9
7
5
4
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
168
100
74
50
49
32
30
28
27
25
25
25
25
25
24
24
24
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
887
854
624
323
285
230
220
190
137
118
96
68
29
29
26
22
20
18
14
9
7
7
6
6
6
6
5
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN119944
$ 665.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN119943 EIA Func Mouse IgG1 Log in to see 4F4
1 ABIN1981885 Func IHC (f) ICC FACS ELISA FITC Mouse IgG1 Log in to see MAb11 5
1 ABIN1690740 ELISA FACS Func ICC IHC (f) PE Mouse IgG1 Log in to see MAb11 5
1 ABIN2476867 Func ELISA WB Mouse IgG1 Log in to see 6E10 3
1 ABIN2476866 Func ELISA Mouse IgG1 Log in to see 4F4 1
1 ABIN3200802 ELISA FACS Func ICC IHC (fro) FITC Mouse IgG1 Log in to see MAb11
1 ABIN3200991 ELISA FACS Func ICC IHC (fro) APC Mouse IgG1 Log in to see MAb11
1 ABIN3200803 ELISA FACS Func ICC IHC (fro) PE Mouse IgG1 Log in to see MAb11
1 ABIN2749165 Func IHC (f) ICC FACS ELISA APC Mouse IgG1 Log in to see MAb11 4
1 ABIN2749097 Func IHC (f) ICC FACS ELISA Mouse IgG1 Log in to see MAb11 4
1 ABIN3200801 ELISA FACS Func ICC IHC (fro) Mouse IgG1 Log in to see MAb11
1 ABIN1959983 BR ELISA FACS Func ICC IHC (fro) Neut Mouse IgG1 Log in to see MAb11
1 ABIN5540920 FACS Func ICC IF IHC (fro) Mouse IgG1 Log in to see Mab11
1 ABIN217733 Func ELISA WB Mouse IgG1 Log in to see
1 ABIN217732 Func ELISA Mouse IgG1 Log in to see
1 ABIN640991 Func ELISA WB Mouse Log in to see
1 ABIN639478 Func ELISA Mouse Log in to see

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Pig (Porcine)
(1253), (462), (357), (144), (136), (119), (118), (61), (54), (41), (29), (17), (14), (12), (9), (7), (6), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Host Mouse
(936), (520), (184), (46), (10), (9), (7), (5), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(168), (100), (74), (50), (49), (32), (30), (28), (27), (25), (25), (25), (25), (25), (24), (24), (24), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
(887), (854), (624), (323), (285), (230), (220), (190), (137), (118), (96), (68), (29), (29), (26), (22), (20), (18), (14), (9), (7), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN119944) Images
Specificity This antibody is specific for tumour necrosis factor- alpha (TNF alpha). The antibody recognises both native and recombinant TNF alpha. Clone 6E10 is reported to neutralise the effects of porcine TNF alpha in in vitro cytotoxicity assays using WEHI 164 clone 13 murine fibrosarcoma cells.
Purification Affinity Chromatography on Protein A
Immunogen Recombinant porcine TNF alpha.
Clone 6-00E-010
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN119944) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background TNF alpha is expressed by a range of cells including macrophages and monocytes, and is an important mediator of the porcine inflammatory response.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 397086
NCBI Accession NP_999187
UniProt P23563
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN119944) Images back to top
Application Notes ELISA. Western Blot. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.015 EU/ug
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN119944) Images back to top
Concentration 0.5 mg/mL
Buffer PBS without preservatives
Preservative Without preservative
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody at 2 - 8 °C. DO NOT FREEZE!

References for anti-Tumor Necrosis Factor antibody (ABIN119944)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Zelnickova, Faldyna, Stepanova, Ondracek, Kovaru: "Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining." in: Journal of immunological methods, Vol. 327, Issue 1-2, pp. 18-29, 2007

Su, Prestwood, Otto, Prickett, McGraw: "Monoclonal antibodies to porcine tumor necrosis factor alpha: development of an enzyme-linked immunosorbent assay." in: Journal of immunoassay, Vol. 13, Issue 2, pp. 253-72, 1992