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anti-Pig (Porcine) Tumor Necrosis Factor antibody for Functional Studies

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Pig (Porcine)
1233
462
344
144
138
123
123
56
51
45
31
18
8
7
5
4
4
4
3
3
3
2
2
1
1
1
Host
Mouse
952
479
210
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
98
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
879
797
577
252
249
163
145
142
135
121
102
62
25
25
23
22
22
17
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN119944
$ 643.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087742624 ABIN119943 EIA Func Mouse IgG1 Log in to see 4F4
0.00087742624 ABIN1690740 Func ICC IHC (fro) FACS ELISA PE Mouse IgG1 Log in to see MAb11 5
0.00087742624 ABIN1981885 Func IHC (f) ICC FACS ELISA FITC Mouse IgG1 Log in to see MAb11 5
0.00087742624 ABIN2749097 Func IHC (f) ICC FACS ELISA Mouse IgG1 Log in to see MAb11 4
0.00087742624 ABIN2749165 Func IHC (f) ICC FACS ELISA APC Mouse IgG1 Log in to see MAb11 4
0.00087742624 ABIN2476866 Func ELISA Mouse IgG1 Log in to see 4F4 1
0.00087742624 ABIN2476867 Func ELISA WB Mouse IgG1 Log in to see 6E10 3
0.00087742624 ABIN254428 Func ELISA WB Mouse IgG1 Log in to see 6-E10
0.00087742624 ABIN3200802 ELISA FACS Func ICC IHC (fro) FITC Mouse IgG1 Log in to see MAb11
0.00087742624 ABIN3200991 ELISA FACS Func ICC IHC (fro) APC Mouse IgG1 Log in to see MAb11
0.00087742624 ABIN3200803 ELISA FACS Func ICC IHC (fro) PE Mouse IgG1 Log in to see MAb11
0.00087742624 ABIN3200801 ELISA FACS Func ICC IHC (fro) Mouse IgG1 Log in to see MAb11
0.00087742624 ABIN217733 Func ELISA WB Mouse IgG1 Log in to see
0.00087742624 ABIN217732 Func ELISA Mouse IgG1 Log in to see
0.00087742624 ABIN3040095 Func ICC FACS IF IHC ELISA Mouse IgG1 Log in to see
0.00087742624 ABIN3040097 Func ICC FACS IF IHC ELISA APC Mouse IgG1 Log in to see
0.00087742624 ABIN3040098 Func ICC FACS IF IHC ELISA FITC Mouse IgG1 Log in to see
0.00087742624 ABIN3040099 Func ICC FACS IF IHC ELISA PE Mouse IgG1 Log in to see
0.00087742624 ABIN639478 Func ELISA Mouse Log in to see
0.00087742624 ABIN640991 Func ELISA WB Mouse Log in to see

Top referenced anti-Tumor Necrosis Factor antibody for Functional Studies

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Pig (Porcine)
ELISA 42 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 15 Antibodies
Functional Studies (Func) 21 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 28 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Pig (Porcine)
(1233), (462), (344), (144), (138), (123), (123), (56), (51), (45), (31), (18), (8), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(952), (479), (210), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (98), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
(879), (797), (577), (252), (249), (163), (145), (142), (135), (121), (102), (62), (25), (25), (23), (22), (22), (17), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN119944) Images
Specificity This antibody is specific for tumour necrosis factor- alpha (TNF alpha). The antibody recognises both native and recombinant TNF alpha. Clone 6E10 is reported to neutralise the effects of porcine TNF alpha in in vitro cytotoxicity assays using WEHI 164 clone 13 murine fibrosarcoma cells.
Purification Affinity Chromatography on Protein A
Immunogen Recombinant porcine TNF alpha.
Clone 6-00E-010
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN119944) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background TNF alpha is expressed by a range of cells including macrophages and monocytes, and is an important mediator of the porcine inflammatory response.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 397086
NCBI Accession NP_999187
UniProt P23563
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN119944) Images back to top
Application Notes ELISA. Western Blot. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.015 EU/ug
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN119944) Images back to top
Concentration 0.5 mg/mL
Buffer PBS without preservatives
Preservative Without preservative
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody at 2 - 8 °C. DO NOT FREEZE!

References for anti-Tumor Necrosis Factor Antibody (ABIN119944)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Zelnickova, Faldyna, Stepanova, Ondracek, Kovaru: "Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining." in: Journal of immunological methods, Vol. 327, Issue 1-2, pp. 18-29, 2007

Su, Prestwood, Otto, Prickett, McGraw: "Monoclonal antibodies to porcine tumor necrosis factor alpha: development of an enzyme-linked immunosorbent assay." in: Journal of immunoassay, Vol. 13, Issue 2, pp. 253-72, 1992