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anti-Pig (Porcine) Tumor Necrosis Factor antibody for Immunofluorescence

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human, Monkey, Pig (Porcine), Baboon
1233
462
344
144
138
123
123
56
52
45
31
18
8
7
5
4
4
4
3
3
3
2
2
1
1
1
Host
Mouse
953
479
210
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to FITC
172
98
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
880
797
577
252
249
163
145
142
135
121
103
63
25
25
23
22
22
17
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN263070
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010843398 ABIN263069 ICC FACS IF ELISA Biotin Mouse IgG1 kappa Log in to see MAb11
0.0010843398 ABIN2506723 ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
0.0010843398 ABIN263068 ICC FACS IHC (fro) IF IHC (p) IHC Mouse IgG1 kappa Log in to see MAb11
0.0010843398 ABIN1172404 ICC IF IHC WB FITC Rabbit IgG Log in to see Polyclonal
0.0010843398 ABIN3040095 Func ICC FACS IF IHC ELISA Mouse IgG1 Log in to see
0.0010843398 ABIN3040097 Func ICC FACS IF IHC ELISA APC Mouse IgG1 Log in to see
0.0010843398 ABIN3040098 Func ICC FACS IF IHC ELISA FITC Mouse IgG1 Log in to see
0.0010843398 ABIN3040099 Func ICC FACS IF IHC ELISA PE Mouse IgG1 Log in to see

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Pig (Porcine) Monkey
ELISA 42 Antibodies 16 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 8 Antibodies
Flow Cytometry (FACS) 15 Antibodies 13 Antibodies
Functional Studies (Func) 21 Antibodies 14 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 28 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human, Monkey, Pig (Porcine), Baboon
(1233), (462), (344), (144), (138), (123), (123), (56), (52), (45), (31), (18), (8), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(953), (479), (210), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to FITC
(172), (98), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(880), (797), (577), (252), (249), (163), (145), (142), (135), (121), (103), (63), (25), (25), (23), (22), (22), (17), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263070) Images
Specificity TNF- alpha (MAb11)
Cross-Reactivity (Details) Predicted cross-reactivity based on sequence identity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Sooty Mangabey, Swine (Pig, Porcine).
Purification Affinity purified
Immunogen E. coli-expressed, recombinant human TNF- alpha
Clone MAb11
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263070) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background TNF- alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principallyCD4+), and NK-cells. Many transformed cell lines also secrete TNF- alpha. Monomerichuman TNF- alpha is a 157 amino acid protein (non-glycosylated) with a reportedmolecular weight of 17 kD. TNF- alpha forms multimeric complexes, stable trimers aremost common in solution. A 26 kD membrane form of TNF- alpha has also beendescribed. TNF- alpha binding to surface receptors elicits a wide array of biologicactivities including: cytolysis and cytostasis of many tumor cell lines in vitro,hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhancedchemotaxis and phagocytosis in neutrophils. The MAb11 antibody can neutralize thebioactivity of natural or recombinant TNF- alpha. Alternate Names: anti-Tumor necrosis factor-alpha antibody, anti-Cachectin antibody, anti-Necrosinantibody, anti-Macrophage cytotoxic factor (MCF) antibody, anti-Differentiation inducingfactor (DIF) antibody, anti-TNFSF2 antibody, anti-TNF-a antibody, anti-TNF-alphaantibody.
Gene Symbol: TNF
Gene ID 7124
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263070) Images back to top
Application Notes The purified MAb11 has been tested by blocking fluorochrome conjugated MAb11 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody ( le 5.0 µg/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunohistochemistry-Paraffin
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN263070) Images back to top
Concentration 1 µL/tests
Buffer PBS, pH 7.2, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C

References for anti-Tumor Necrosis Factor Antibody (ABIN263070)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Simon, Aeberhard, Erdemoglu, Simon: "Th17 cells and tissue remodeling in atopic and contact dermatitis." in: Allergy, Vol. 69, Issue 1, pp. 125-31, 2014 (Sample species: Human). Further details: Immunocytochemistry,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263070) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor antibody (TNF)  (FITC) (ABIN263070) PMA/Ionomycin-stimulated human PBMCs were stained with CD3 PE/Cy5 and MAb11 PE