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anti-Primate Tumor Necrosis Factor antibody for Western Blotting

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human, Mouse (Murine), Primate, Rat (Rattus)
1242
465
346
144
137
123
123
56
56
45
31
18
8
8
7
6
4
4
4
3
2
2
2
1
1
1
Host
Rabbit
959
479
213
43
13
9
9
8
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
99
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
881
804
582
258
254
168
146
146
136
125
104
63
25
25
24
22
22
19
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN153474
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011817255 ABIN4361149 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.0011817255 ABIN4360907 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.0011817255 ABIN2497066 ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-Tumor Necrosis Factor antibody for Western Blotting

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 70 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 2 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 143 Antibodies
Functional Studies (Func) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 68 Antibodies
Immunofluorescence (IF) 102 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 65 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human, Mouse (Murine), Primate, Rat (Rattus)
(1242), (465), (346), (144), (137), (123), (123), (56), (56), (45), (31), (18), (8), (8), (7), (6), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(959), (479), (213), (43), (13), (9), (9), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (99), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(881), (804), (582), (258), (254), (168), (146), (146), (136), (125), (104), (63), (25), (25), (24), (22), (22), (19), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN153474) Images
Specificity The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNG beta (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Purification Delipidation and Defibrination
Immunogen Recombinant full length protein produced in E. coli (Human).

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN153474) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Gene ID 7124
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN153474) Images back to top
Application Notes Western Blot 1:500 - 1:2000, ELISA 1:1000 - 1:5000, Immunohistochemistry 1:100, Immunohistochemistry-Paraffin 1:100
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN153474) Images back to top
Format Liquid
Concentration 75 mg/mL
Buffer Whole antisera.
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Tumor Necrosis Factor Antibody (ABIN153474)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Rossi, Cadamuro, Biselli-Périco, Leite, Severino, Reis, Cordeiro, Silva: "Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection." in: Cellular microbiology, Vol. 18, Issue 10, pp. 1444-58, 2016 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Chen, Jian, Lien, Lin, Ting, Chen, Hsu, Huang, Wu, Kuan, Chao, Wu, Huang, Juan: "Adipocytes play an etiological role in the podocytopathy of high-fat diet-fed rats." in: The Journal of endocrinology, 2016 (Sample species: Rat (Rattus)). Further details: Western Blotting

Chen, Zhang, Wang, Lei, Su, Zeng, Pei, Huang: "Inhibition of immunoproteasome reduces infarction volume and attenuates inflammatory reaction in a rat model of ischemic stroke." in: Cell death & disease, Vol. 6, pp. e1626, 2015 (Sample species: Rat (Rattus)). Further details: Western Blotting

Tessier, Raha, Holloway, Yockell-Lelièvre, Tayade, Gruslin: "Characterization of immune cells and cytokine localization in the rat utero-placental unit mid- to late gestation." in: Journal of reproductive immunology, Vol. 110, pp. 89-101, 2015 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Wang, Parrish: "Loss of α(E)-catenin promotes Fas mediated apoptosis in tubular epithelial cells." in: Apoptosis : an international journal on programmed cell death, Vol. 20, Issue 7, pp. 921-9, 2015 (Sample species: Rat (Rattus)). Further details: Western Blotting

Rodriguez, Edrey, Osmulski, Gaczynska, Buffenstein: "Altered composition of liver proteasome assemblies contributes to enhanced proteasome activity in the exceptionally long-lived naked mole-rat." in: PLoS ONE, Vol. 7, Issue 5, pp. e35890, 2012 Further details: Western Blotting

Roth, Städler, Lemann, Hösli, Simon, Simon: "Distinct eosinophil cytokine expression patterns in skin diseases - the possible existence of functionally different eosinophil subpopulations." in: Allergy, Vol. 66, Issue 11, pp. 1477-86, 2011 (Sample species: Human). Further details: Immunohistochemistry

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN153474) back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN153474) anti-Tumor Necrosis Factor (TNF) antibody
Western Blotting (WB) image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN153474) Western Blot: TNF-alpha Antibody [ABIN153474] - Analysis using Azide/BSA FREE version...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN153474) Immunohistochemistry: TNF-alpha Antibody [ABIN153474] - Staining of formalin/PFA-fixe...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN153474) Immunohistochemistry-Paraffin: TNF-alpha Antibody [ABIN153474] - Analysis using Azide...