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anti-Rabbit Tumor Necrosis Factor antibody for Immunocytochemistry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Epitope
AA 80-235
35
30
28
25
24
23
18
17
13
12
10
10
10
7
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Rabbit
1243
466
347
144
136
123
123
56
56
45
31
18
8
8
7
7
4
4
4
3
2
2
2
1
1
1
Host
Guinea Pig
959
480
213
43
13
9
8
8
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
99
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
881
803
582
258
255
167
146
146
136
124
104
63
25
25
24
22
22
19
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1860789
$ 292.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000999342 ABIN1860790 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 80-235 Log in to see
0.000999342 ABIN4360942 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.000999342 ABIN4360941 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.000999342 ABIN4360943 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.000999342 ABIN4360935 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8
0.000999342 ABIN4360936 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8
0.000999342 ABIN4360944 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM Log in to see TNF706-P-T2
0.000999342 ABIN4360957 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see SPM543
0.000999342 ABIN4360940 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see P-T2
0.000999342 ABIN4360971 ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.000999342 ABIN4360958 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see SPM543
0.000999342 ABIN4360972 ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.000999342 ABIN4360977 FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM543
0.000999342 ABIN4360979 FACS ICC IF IHC (fro) IHC (p) IP Allophycocyanin Mouse IgG1 kappa Log in to see SPM543
0.000999342 ABIN4360981 FACS ICC IF IHC (fro) IHC (p) IP DyLight 650 Mouse IgG1 kappa Log in to see SPM543
0.000999342 ABIN4360982 FACS ICC IF IHC (fro) IHC (p) IP DyLight 680 Mouse IgG1 kappa Log in to see SPM543
0.000999342 ABIN4360983 FACS ICC IF IHC (fro) IHC (p) IP DyLight 488 Mouse IgG1 kappa Log in to see SPM543
0.000999342 ABIN4360989 FACS ICC IF IHC (fro) IHC (p) IP DyLight 350 Mouse IgG1 kappa Log in to see SPM543
0.000999342 ABIN4360990 FACS ICC IF IHC (fro) IHC (p) IP DyLight 405 Mouse IgG1 kappa Log in to see SPM543
0.000999342 ABIN4360988 FACS ICC IF IHC (fro) IHC (p) IP DyLight 550 Mouse IgG1 kappa Log in to see SPM543

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rabbit
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 18 Antibodies
Flow Cytometry (FACS) 52 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 45 Antibodies
Immunofluorescence (IF) 53 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 44 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 51 Antibodies
Immunoprecipitation (IP) 43 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 78 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 80-235
(35), (30), (28), (25), (24), (23), (18), (17), (13), (12), (10), (10), (10), (7), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Rabbit
(1243), (466), (347), (144), (136), (123), (123), (56), (56), (45), (31), (18), (8), (8), (7), (7), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Guinea Pig
(959), (480), (213), (43), (13), (9), (8), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (99), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(881), (803), (582), (258), (255), (167), (146), (146), (136), (124), (104), (63), (25), (25), (24), (22), (22), (19), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- L RSASRALSDK PLAHVVANPQ VEGQLQWLSQ RANALLANGM KLTDNQLVVP ADGLYLIYSQ VLFSGQGCRS YVLLTHTVSR FAVSYPNKVN LLSAIKSPCH RETPEEAEPM AWYEPIYLGG VFQLEKGDRL STEVNQPEYL DLAESGQVYF GIIAL
Specificity It has been selected for its ability to recognize TNFa in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen TNFa (AA 80-235)
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name Tumor Necrosis Factor Alpha (TNFa) (TNF Antibody Abstract)
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant TNFa (Leu80~Leu235) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 80-235) (ABIN1860789) anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody