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anti-Rabbit Tumor Necrosis Factor antibody for Immunofluorescence

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Epitope
AA 115-130
37
28
25
17
13
12
10
10
10
7
7
6
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
704
307
141
55
52
31
30
26
23
18
11
11
8
7
5
4
4
4
3
3
2
2
1
1
1
Host
Mouse
537
394
157
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
147
83
44
24
20
9
9
7
5
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
633
587
318
207
120
100
99
86
80
63
56
55
25
25
22
22
15
14
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN3026343
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010796441 ABIN3023991 FACS IF IHC Mouse IgG1, kappa AA 115-130 Log in to see SPM543
0.0010796441 ABIN3023960 FACS IF IHC (p) Mouse IgM AA 115-130, N-Term Log in to see TNF706-P-T2
0.0010796441 ABIN3026344 FACS IF IHC Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8
0.0010796441 ABIN3023987 FACS IF IHC (p) Mouse IgM, kappa AA 115-130 Log in to see P-T2
0.0010796441 ABIN3023989 FACS IF IHC (p) Mouse IgM, kappa N-Term Log in to see TNF706
0.0010796441 ABIN3023992 FACS IF IHC Mouse IgG1, kappa AA 115-130 Log in to see SPM543
0.0010796441 ABIN3023962 FACS IF IHC (p) Mouse IgM AA 115-130, N-Term Log in to see TNF706-P-T2
0.0010796441 ABIN3023988 FACS IF IHC (p) Mouse IgM, kappa AA 115-130 Log in to see P-T2
0.0010796441 ABIN3023990 FACS IF IHC (p) Mouse IgM, kappa N-Term Log in to see TNF706
0.0010796441 ABIN1172410 ICC IF IHC WB FITC Guinea Pig Log in to see Polyclonal

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Rabbit Cat (Feline) Zebrafish (Danio rerio)
Western Blotting (WB) 325 Antibodies 149 Antibodies 73 Antibodies 9 Antibodies 11 Antibodies 1 Antibodies 1 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Radioimmunoassay (RIA) 6 Antibodies 17 Antibodies
Protein Assay (PrA) 2 Antibodies
Neutralization (Neut) 52 Antibodies 53 Antibodies 21 Antibodies 1 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies 1 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Intracellular Staining (ICS) 12 Antibodies 12 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 115-130
(37), (28), (25), (17), (13), (12), (10), (10), (10), (7), (7), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(704), (307), (141), (55), (52), (31), (30), (26), (23), (18), (11), (11), (8), (7), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(537), (394), (157), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(147), (83), (44), (24), (20), (9), (9), (7), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
(633), (587), (318), (207), (120), (100), (99), (86), (80), (63), (56), (55), (25), (25), (22), (22), (15), (14), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Characteristics This antibody is specific for a 17-26 kDa protein, which is identified as the cytokine TNF alpha (Tumor Necrosis Factor alpha). It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. It is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. It exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Purity PEG precipitation
Immunogen A hexadecapeptide corresponding to aa 115-130 of human TNF alpha, conjugated to thyroglobulin, was used as the immunogen for this TNF alpha antibody.
Clone 4C6-H8
Isotype IgM, kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF alpha (TNF Antibody Abstract)
Gene ID 7124
Research Area Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (Formalin-paraffin): 1-2 μg/mL for 30 min at RT (1)

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the TNF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).