anti-Rabbit Tumor Necrosis Factor antibody for Immunohistochemistry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Epitope
AA 115-130
44
39
29
27
24
22
17
16
15
11
11
10
10
8
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
1229
444
357
144
135
118
118
62
55
41
29
18
13
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Mouse
928
490
182
48
10
9
7
6
3
2
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
165
97
71
50
32
30
27
26
25
25
25
25
25
25
24
24
24
20
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
870
853
599
311
272
230
201
181
137
118
98
70
27
26
25
22
20
16
14
8
7
6
6
6
6
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN3023991
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4360908 FACS IHC IHC (fro) IHC (p) WB FITC Mouse IgG1 Log in to see 52B83 1
1 ABIN1860789 ICC IHC IP WB Guinea Pig IgG AA 80-235 Log in to see Polyclonal
1 ABIN3026343 FACS IF IHC Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8
1 ABIN2380220 IC IHC ELISA WB Guinea Pig IgG AA 80-235 Log in to see Polyclonal
1 ABIN3026344 FACS IF IHC Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8
1 ABIN2380223 IC IHC ELISA WB Mouse AA 80-235 Log in to see
1 ABIN3023961 IHC Mouse IgM AA 115-130, N-Term Log in to see TNF706-P-T2
1 ABIN1172409 IHC ELISA WB Biotin Guinea Pig IgG Log in to see Polyclonal
1 ABIN3023992 FACS IF IHC Mouse IgG1, kappa AA 115-130 Log in to see SPM543
1 ABIN2904353 ELISA IF/ICC IHC WB Guinea Pig AA 80-235 Log in to see Polyclonal
1 ABIN2904356 ELISA IF/ICC IHC WB Mouse AA 80-235 Log in to see
1 ABIN1078628 IHC ELISA WB Guinea Pig Log in to see Polyclonal
1 ABIN1172410 ICC IF IHC WB FITC Guinea Pig Log in to see Polyclonal

Top referenced anti-Tumor Necrosis Factor antibody for Immunohistochemistry

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Rabbit Dog (Canine) Cat (Feline) Zebrafish
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies 3 Antibodies 3 Antibodies 2 Antibodies 2 Antibodies
Activity Inhibition (Act Inh) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 27 Antibodies 10 Antibodies 10 Antibodies 10 Antibodies 10 Antibodies 18 Antibodies 10 Antibodies
Immunohistochemistry (Free Floating) (IHC (ff)) 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 538 Antibodies 252 Antibodies 157 Antibodies 66 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 115-130
(44), (39), (29), (27), (24), (22), (17), (16), (15), (11), (11), (10), (10), (8), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(1229), (444), (357), (144), (135), (118), (118), (62), (55), (41), (29), (18), (13), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Mouse
(928), (490), (182), (48), (10), (9), (7), (6), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(165), (97), (71), (50), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (20), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
(870), (853), (599), (311), (272), (230), (201), (181), (137), (118), (98), (70), (27), (26), (25), (22), (20), (16), (14), (8), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Amino acids 115-130 of the human protein was used as the immunogen for this anti-TNF alpha antibody.
Clone SPM543
Isotype IgG1, kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes The optimal dilution of the anti-TNF-alpha antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the anti-TNF-alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 115-130) (ABIN3023991) Formalin-fixed, paraffin-embedded human polyostotic sclerosing histiocytosis (Erdheim...