|Application / Reactivity||Rat (Rattus)|
|Blocking Antibody (Inhibition)||3 Antibodies|
|Blocking Reagent (BR)||1 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISA (Detection)||1 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||32 Antibodies|
|Functional Studies (Func)||8 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies|
|Immunofluorescence (IF)||25 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||43 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||24 Antibodies|
|Antigen||Tumor Necrosis Factor (TNF) Antibodies|
|Reactivity||Mouse (Murine), Rabbit, Rat (Rattus) Alternatives|
|Host||Armenian Hamster Alternatives|
|Conjugate||This Tumor Necrosis Factor antibody is un-conjugated Alternatives|
Blocking Antibody (Inhibition), Immunoprecipitation (IP), Neutralization (Neut), ELISA, Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-Tumor Necrosis Factor AntibodyTarget Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2689936) Images
The TN3-19.12 antibody reacts with rat and mouse tumor-necrosis factor (TNF) proteins (also known as TNF-α) . Moreover, the TN3-19.12 antibody is reported to crossreact with rabbit TNF, but it does not crossreact with mouse lymphotoxin-α (LT-α, also known as TNF-β) nor with human TNF. The immunogen used to generate the TN3-19.12 hybridoma was E. coli-expressed, purified recombinant mouse TNF protein. This monoclonal antibody has been reported to neutralize the bioactivities of mouse, rat and rabbit TNF. This antibody is routinely tested by ELISA. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Purified Hamster Anti-Mouse/Rat TNF - Purified - Clone TN3-19.12 - Isotype Armenian Hamster IgG1, λ1 - Reactivity Ms, Rat, Rab - 0.5 mg
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Recombinant mouse TNF protein|
Target Details Tumor Necrosis FactorProduct Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2689936) Images back to top
|Alternative Name||TNF (TNF Antibody Abstract)|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2689936) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN2689936) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-Tumor Necrosis Factor Antibody (ABIN2689936)Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
|Product cited in:||
Takahashi, Kapás, Fang et al.: "An anti-tumor necrosis factor antibody suppresses sleep in rats and rabbits." in: Brain research, Vol. 690, Issue 2, pp. 241-4, 1996 (PubMed).
Rabinovici, Bugelski, Esser et al.: "Tumor necrosis factor-alpha mediates endotoxin-induced lung injury in platelet activating factor-primed rats." in: The Journal of pharmacology and experimental therapeutics, Vol. 267, Issue 3, pp. 1550-7, 1994 (PubMed).
Ferran, Dautry, Mérite et al.: "Anti-tumor necrosis factor modulates anti-CD3-triggered T cell cytokine gene expression in vivo." in: The Journal of clinical investigation, Vol. 93, Issue 5, pp. 2189-96, 1994 (PubMed).
Sheehan, Ruddle, Schreiber: "Generation and characterization of hamster monoclonal antibodies that neutralize murine tumor necrosis factors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 11, pp. 3884-93, 1989 (PubMed).
Leiby, Fortier, Crawford et al.: "In vivo modulation of the murine immune response to Francisella tularensis LVS by administration of anticytokine antibodies." in: Infection and immunity, Vol. 60, Issue 1, pp. 84-9, 1992 (PubMed).
Merrick, He, Craig et al.: "Two dimensional gel electrophoresis of cellular and secreted proteins from rat alveolar macrophages after lipopolysaccharide treatment." in: Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society, Vol. 2, Issue 6, pp. 177-87, 1992 (PubMed).