anti-Rabbit Tumor Necrosis Factor antibody for Western Blotting

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Epitope
AA 80-235
50
44
29
27
24
22
19
17
15
11
11
10
10
8
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Rabbit
1253
462
357
144
135
119
118
62
54
41
29
17
14
12
9
7
6
4
4
4
3
2
1
1
1
1
Host
Mouse
936
520
184
46
10
9
7
5
4
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
168
100
74
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
886
854
624
322
285
229
220
189
137
118
97
69
29
29
26
22
20
18
14
9
7
7
6
6
6
6
5
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN5662008
$ 203.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.226648 ABIN1860789 ICC IHC IP WB Guinea Pig IgG AA 80-235 Log in to see Polyclonal
1 ABIN4360908 FACS IHC IHC (fro) IHC (p) WB FITC Mouse IgG1 Log in to see 52B83 1
1 ABIN5515558 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2689936 Inhibition IP Neut ELISA WB Armenian Hamster IgG1, lambda Log in to see TN3 6
1 ABIN4361077 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361087 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361092 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361085 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361078 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361079 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361080 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361083 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361084 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361088 WB PerCP Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361090 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361091 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4360994 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgM kappa Log in to see 4C6-H8
1 ABIN4360998 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgM kappa Log in to see 4C6-H8
1 ABIN4361000 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgM kappa Log in to see 4C6-H8
1 ABIN4361006 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgM kappa Log in to see 4C6-H8

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 80-235
(50), (44), (29), (27), (24), (22), (19), (17), (15), (11), (11), (10), (10), (8), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rabbit
(1253), (462), (357), (144), (135), (119), (118), (62), (54), (41), (29), (17), (14), (12), (9), (7), (6), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Host Mouse
(936), (520), (184), (46), (10), (9), (7), (5), (4), (3), (2)
Clonality
Monoclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(168), (100), (74), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(886), (854), (624), (322), (285), (229), (220), (189), (137), (118), (97), (69), (29), (29), (26), (22), (20), (18), (14), (9), (7), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- L RSASRALSDK PLAHVVANPQ VEGQLQWLSQ RANALLANGM KLTDNQLVVP ADGLYLIYSQ VLFSGQGCRS YVLLTHTVSR FAVSYPNKVN LLSAIKSPCH RETPEEAEPM AWYEPIYLGG VFQLEKGDRL STEVNQPEYL DLAESGQVYF GIIAL
Specificity It has been selected for its ability to recognize TNFa in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen TNFa (AA 80-235)
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name Tumor Necrosis Factor Alpha (TNFa) (TNF Antibody Abstract)
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Concentration 500 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody (ABIN5662008) anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody