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anti-Rabbit Tumor Necrosis Factor antibody for Western Blotting

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Epitope
AA 80-235
36
30
28
25
23
23
18
16
13
12
10
10
10
7
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Rabbit
1233
462
344
144
137
123
123
56
52
45
31
18
8
7
5
4
4
4
3
3
3
2
2
1
1
1
Host
Guinea Pig
953
479
210
43
13
9
8
7
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
98
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
879
796
577
251
249
162
145
142
135
120
103
63
25
25
23
22
22
17
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN1860789
$ 292.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13433187 ABIN1860790 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 80-235 Log in to see
0.0011739905 ABIN2689936 Inhibition IP Neut ELISA WB Armenian Hamster IgG1, lambda Log in to see TN3 6
0.0011739905 ABIN2629356 ELISA WB Biotin Mouse IgG AA 80-235 Log in to see
0.0011739905 ABIN2629362 WB FITC Guinea Pig IgG AA 80-235 Log in to see Polyclonal
0.0011739905 ABIN2629366 WB FITC Mouse IgG AA 80-235 Log in to see
0.0011739905 ABIN4361077 WB Alexa Fluor 405 Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361078 WB Alexa Fluor 488 Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361079 WB Alexa Fluor 647 Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361080 WB Alexa Fluor 700 Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361081 WB Allophycocyanin Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361082 WB Biotin Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361083 WB DyLight 650 Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361084 WB DyLight 680 Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361085 WB DyLight 488 Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361087 WB DyLight 755 Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361088 WB PerCP Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361089 WB PE Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361090 WB DyLight 550 Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361091 WB DyLight 350 Mouse IgM kappa N-Term Log in to see TNF706
0.0011739905 ABIN4361092 WB DyLight 405 Mouse IgM kappa N-Term Log in to see TNF706

Top referenced anti-Tumor Necrosis Factor antibody for Western Blotting

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rabbit
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 19 Antibodies
Flow Cytometry (FACS) 52 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 45 Antibodies
Immunofluorescence (IF) 53 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 44 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 51 Antibodies
Immunoprecipitation (IP) 43 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 79 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 80-235
(36), (30), (28), (25), (23), (23), (18), (16), (13), (12), (10), (10), (10), (7), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Rabbit
(1233), (462), (344), (144), (137), (123), (123), (56), (52), (45), (31), (18), (8), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Guinea Pig
(953), (479), (210), (43), (13), (9), (8), (7), (4), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (98), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(879), (796), (577), (251), (249), (162), (145), (142), (135), (120), (103), (63), (25), (25), (23), (22), (22), (17), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- L RSASRALSDK PLAHVVANPQ VEGQLQWLSQ RANALLANGM KLTDNQLVVP ADGLYLIYSQ VLFSGQGCRS YVLLTHTVSR FAVSYPNKVN LLSAIKSPCH RETPEEAEPM AWYEPIYLGG VFQLEKGDRL STEVNQPEYL DLAESGQVYF GIIAL
Specificity It has been selected for its ability to recognize TNFa in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen TNFa (AA 80-235)
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name Tumor Necrosis Factor Alpha (TNFa) (TNF Antibody Abstract)
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant TNFa (Leu80~Leu235) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 80-235) (ABIN1860789) anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody