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anti-Rabbit Tumor Necrosis Factor antibody for Western Blotting

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Epitope
AA 80-235
36
30
27
25
23
23
18
16
14
12
10
10
10
7
4
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Rabbit
1200
442
329
139
131
118
118
55
55
31
26
18
8
8
7
7
4
4
4
3
2
2
2
1
1
1
Host
Mouse
914
477
201
44
13
9
7
4
3
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
167
95
69
49
32
30
27
26
25
25
25
25
25
25
24
24
24
18
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
852
796
536
252
251
162
141
140
134
123
101
63
25
24
23
22
18
14
8
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN1860790
$ 319.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12599558 ABIN1860789 ICC IHC (fro) IHC (p) ELISA WB Guinea Pig IgG AA 80-235 Log in to see Polyclonal
0.0012191178 ABIN2689936 Inhibition IP Neut ELISA WB Armenian Hamster IgG1, lambda Log in to see TN3 6
0.0012191178 ABIN4361077 WB Alexa Fluor 405 Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361078 WB Alexa Fluor 488 Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361079 WB Alexa Fluor 647 Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361081 WB Allophycocyanin Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361080 WB Alexa Fluor 700 Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361082 WB Biotin Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361083 WB DyLight 650 Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361084 WB DyLight 680 Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361085 WB DyLight 488 Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361088 WB PerCP Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361087 WB DyLight 755 Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361090 WB DyLight 550 Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361089 WB PE Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361091 WB DyLight 350 Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN4361092 WB DyLight 405 Mouse IgM kappa N-Term Log in to see TNF706
0.0012191178 ABIN2629362 WB FITC Guinea Pig IgG AA 80-235 Log in to see Polyclonal
0.0012191178 ABIN2629366 WB FITC Mouse IgG AA 80-235 Log in to see
0.0012191178 ABIN2380220 IC IHC ELISA WB Guinea Pig IgG AA 80-235 Log in to see Polyclonal

Top referenced anti-Tumor Necrosis Factor antibody for Western Blotting

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rabbit
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 18 Antibodies
Flow Cytometry (FACS) 51 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 44 Antibodies
Immunofluorescence (IF) 52 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 43 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies
Immunoprecipitation (IP) 42 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 74 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 80-235
(36), (30), (27), (25), (23), (23), (18), (16), (14), (12), (10), (10), (10), (7), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Rabbit
(1200), (442), (329), (139), (131), (118), (118), (55), (55), (31), (26), (18), (8), (8), (7), (7), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(914), (477), (201), (44), (13), (9), (7), (4), (3), (1)
Clonality
Monoclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(167), (95), (69), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (18), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(852), (796), (536), (252), (251), (162), (141), (140), (134), (123), (101), (63), (25), (24), (23), (22), (18), (14), (8), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- L RSASRALSDK PLAHVVANPQ VEGQLQWLSQ RANALLANGM KLTDNQLVVP ADGLYLIYSQ VLFSGQGCRS YVLLTHTVSR FAVSYPNKVN LLSAIKSPCH RETPEEAEPM AWYEPIYLGG VFQLEKGDRL STEVNQPEYL DLAESGQVYF GIIAL
Specificity It has been selected for its ability to recognize TNFa in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen TNFa (AA 80-235)
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name Tumor Necrosis Factor Alpha (TNFa) (TNF Antibody Abstract)
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Concentration 500 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 80-235) (ABIN1860790) anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody