anti-Rat (Rattus) Tumor Necrosis Factor antibody for Mass Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Mouse (Murine), Rat (Rattus)
1255
459
357
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Goat
940
516
184
47
10
9
7
6
4
3
2
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Biotin
168
100
74
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Mass Cytometry (CyTOF), Immunoprecipitation (IP), Immunohistochemistry (IHC)
887
859
621
322
285
230
218
190
137
117
98
74
29
27
26
22
20
18
14
9
7
7
6
6
6
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN2661299
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Top referenced anti-Tumor Necrosis Factor antibody for Mass Cytometry

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 18 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 128 Antibodies
ELISpot 2 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 197 Antibodies
Functional Studies (Func) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 112 Antibodies
Immunofluorescence (IF) 148 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 61 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(1255), (459), (357), (145), (136), (119), (119), (63), (55), (41), (29), (18), (14), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Goat
(940), (516), (184), (47), (10), (9), (7), (6), (4), (3), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to Biotin
(168), (100), (74), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Mass Cytometry (CyTOF), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(887), (859), (621), (322), (285), (230), (218), (190), (137), (117), (98), (74), (29), (27), (26), (22), (20), (18), (14), (9), (7), (7), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN2661299) Images
Cross-Reactivity Rat (Rattus)
Purification The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Clone Poly5160
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN2661299) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes, stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN2661299) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN2661299) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN2661299)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Tracey, Cerami: "Tumor necrosis factor: an updated review of its biology." in: Critical care medicine, Vol. 21, Issue 10 Suppl, pp. S415-22, 1993

Beutler, Cerami: "The biology of cachectin/TNF--a primary mediator of the host response." in: Annual review of immunology, Vol. 7, pp. 625-55, 1989

Beutler, Cerami: "Tumor necrosis, cachexia, shock, and inflammation: a common mediator." in: Annual review of biochemistry, Vol. 57, pp. 505-18, 1988

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN2661299) back to top
Supplier Images
ELISA image for anti-Tumor Necrosis Factor antibody (TNF)  (Biotin) (ABIN2661299) anti-Tumor Necrosis Factor (TNF) antibody (Biotin)