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anti-Rat (Rattus) Tumor Necrosis Factor antibody for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Mouse (Murine), Rat (Rattus)
1216
442
356
142
135
119
119
56
52
41
28
17
11
9
9
7
6
4
4
4
4
2
1
1
1
Host
Rat
924
482
181
42
10
9
7
6
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
160
95
70
49
32
30
27
26
25
25
25
25
25
25
24
24
24
19
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
865
839
591
306
273
228
201
180
137
119
96
62
27
26
25
22
20
16
14
8
7
6
6
6
5
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN119358
$ 544.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2665893 FACS IHC Hamster IgG Log in to see TN3-19-12 9
1 ABIN2661299 FACS IF CyTOF IP IHC Biotin Goat IgG Log in to see Polyclonal 3
1 ABIN2665424 FACS ICFC Hamster IgG Log in to see TN3-19-12 9
1 ABIN2663939 FACS PE Hamster IgG Log in to see TN3-19-12 9
1 ABIN2476890 FACS PE Rat IgG1 Log in to see MP6-XT22 4
1 ABIN2665892 FACS IHC Hamster IgG Log in to see TN3-19-12 9
1 ABIN217631 FACS IHC (fro) ELISA Rat IgG1 Log in to see
1 ABIN2665423 FACS ICFC Hamster IgG Log in to see TN3-19-12 9
1 ABIN4360942 FACS ICC IF IHC (fro) IHC (p) Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361089 FACS PE Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361081 FACS Allophycocyanin Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361084 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361087 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361135 FACS IF Mouse IgG1 kappa Log in to see J2D10
1 ABIN4360941 FACS ICC IF IHC (fro) IHC (p) Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361078 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361080 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361085 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361090 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361092 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgM kappa N-Term Log in to see TNF706

Top referenced anti-Tumor Necrosis Factor antibody for Flow Cytometry

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 18 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 128 Antibodies
ELISpot 2 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 197 Antibodies
Functional Studies (Func) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 112 Antibodies
Immunofluorescence (IF) 148 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 61 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(1216), (442), (356), (142), (135), (119), (119), (56), (52), (41), (28), (17), (11), (9), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1)
Host Rat
(924), (482), (181), (42), (10), (9), (7), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(160), (95), (70), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (19), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(865), (839), (591), (306), (273), (228), (201), (180), (137), (119), (96), (62), (27), (26), (25), (22), (20), (16), (14), (8), (7), (6), (6), (6), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN119358) Images
Purification Affinity Chromatography on Protein G
Endotoxin Level less than 0.01 EU/μg
Immunogen Recombinant Murine TNF alpha expressed in E. coli.
Clone MP6-XT22
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN119358) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background TNF alpha is a 17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immuno-regulatory role.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 10090
UniProt P06804
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN119358) Images back to top
Application Notes Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. ELISA: 1 μg/mL - 10 μg/mL. Functional Assays. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN119358) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 without preservatives., None, None
Preservative Without preservative
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody at 2 - 8 °C. DO NOT FREEZE!

References for anti-Tumor Necrosis Factor Antibody (ABIN119358)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Srinivas, Lovell, Skipper, Bennington, Kurban, Smith: "Ammonia absorption in calcium graphite intercalation compound: in situ neutron diffraction, Raman spectroscopy and magnetization." in: Physical chemistry chemical physics : PCCP, Vol. 12, Issue 23, pp. 6253-9, 2010

Ruan, Kang, Pei, Le: "Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease." in: Current Alzheimer research, Vol. 6, Issue 6, pp. 531-40, 2009

Hostager, Bishop: "Role of TNF receptor-associated factor 2 in the activation of IgM secretion by CD40 and CD120b." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 7, pp. 3318-22, 2002

Scanga, Mohan, Joseph, Yu, Chan, Flynn: "Reactivation of latent tuberculosis: variations on the Cornell murine model." in: Infection and immunity, Vol. 67, Issue 9, pp. 4531-8, 1999

Ross, Williams, Mason, Mauri, Marinova-Mutafchieva, Malfait, Maini, Feldmann: "Suppression of TNF-alpha expression, inhibition of Th1 activity, and amelioration of collagen-induced arthritis by rolipram." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 12, pp. 6253-9, 1998

Yang, Kawamura, Zhu, Mitsuyama: "Involvement of natural killer cells in nitric oxide production by spleen cells after stimulation with Mycobacterium bovis BCG. Study of the mechanism of the different abilities of viable and killed BCG." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 12, pp. 5728-35, 1996

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992