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anti-Rat (Rattus) Tumor Necrosis Factor antibody for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Mouse (Murine), Rat (Rattus)
1242
464
345
144
138
123
123
56
56
45
31
18
8
8
7
7
4
4
4
3
3
2
2
1
1
1
Host
Armenian Hamster
960
480
213
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to EFluor 660
173
99
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
883
805
581
258
255
167
146
145
136
124
104
63
25
24
24
22
22
19
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2681112
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010482287 ABIN119358 EIA Func FACS IHC (fro) IHC (p) Rat IgG1 Log in to see MP6-XT22 7
0.0010482287 ABIN2661299 FACS IF CyTOF IP IHC Biotin Goat IgG Log in to see Polyclonal 3
0.0010482287 ABIN2665893 FACS IHC Hamster IgG Log in to see TN3-19-12 9
0.0010482287 ABIN153604 FACS FITC Rat IgG1 Log in to see MP6-XT22
0.0010482287 ABIN2665424 FACS ICFC Hamster IgG Log in to see TN3-19-12 9
0.0010482287 ABIN2476890 FACS PE Rat IgG1 Log in to see MP6-XT22 4
0.0010482287 ABIN2663939 FACS PE Hamster IgG Log in to see TN3-19-12 9
0.0010482287 ABIN2665892 FACS IHC Hamster IgG Log in to see TN3-19-12 9
0.0010482287 ABIN2665423 FACS ICFC Hamster IgG Log in to see TN3-19-12 9
0.0010482287 ABIN2476887 FACS FITC Rat IgG1 Log in to see MP6-XT22 4
0.0010482287 ABIN2476889 Func IHC (fro) FACS ELISA Rat IgG1 Log in to see MP6-XT22 4
0.0010482287 ABIN4361133 FACS IF Mouse IgG1 kappa Log in to see J2D10
0.0010482287 ABIN4361134 FACS IF Mouse IgG1 kappa Log in to see J2D10
0.0010482287 ABIN4361135 FACS IF Mouse IgG1 kappa Log in to see J2D10
0.0010482287 ABIN4360941 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.0010482287 ABIN4360943 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.0010482287 ABIN4360942 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.0010482287 ABIN283063 FACS ICS Neut ELISA PE-Cy7 Armenian Hamster IgG Log in to see TN3-19-12
0.0010482287 ABIN2476885 IHC (fro) FACS ELISA Rat IgG1 Log in to see MP6-XT22 4
0.0010482287 ABIN3026343 FACS IF IHC Mouse IgM, kappa AA 115-130 Log in to see 4C6-H8

Top referenced anti-Tumor Necrosis Factor antibody for Flow Cytometry

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 70 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 2 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 144 Antibodies
Functional Studies (Func) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 67 Antibodies
Immunofluorescence (IF) 101 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(1242), (464), (345), (144), (138), (123), (123), (56), (56), (45), (31), (18), (8), (8), (7), (7), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Armenian Hamster
(960), (480), (213), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to EFluor 660
(173), (99), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(883), (805), (581), (258), (255), (167), (146), (145), (136), (124), (104), (63), (25), (24), (24), (22), (22), (19), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor alpha (TNF alpha), but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.
Characteristics The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor alpha (TNF alpha), but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.
Synonyms: Synonyms: Tumor necrosis factor
Clone TN3-19-12
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF alpha (TNF Antibody Abstract)
Research Area Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Applications reported: This TN3-19.12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This TN3-19.12 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splecnoytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Concentration 0.2 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor antibody (TNF)  (EFluor 660) (ABIN2681112) anti-Tumor Necrosis Factor (TNF) antibody (EFluor 660)