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anti-Rat (Rattus) Tumor Necrosis Factor antibody for Immunocytochemistry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Epitope
AA 80-235
35
30
28
25
24
23
18
17
13
12
10
10
10
7
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Rat (Rattus)
1243
466
346
144
137
123
123
56
56
45
31
18
8
8
7
7
4
4
4
3
2
2
2
1
1
1
Host
Rabbit
959
479
213
43
13
9
9
8
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
99
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
881
803
582
258
255
167
146
146
136
124
104
63
25
25
24
22
22
19
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN1078627
$ 292.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000999302 ABIN3201322 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 80-235 Log in to see 1
0.000999302 ABIN446837 ICC IF IHC IHC (ff) IHC (fro) IHC (p) WB Rabbit AA 100-200, Internal Region Log in to see Polyclonal 11
0.000999302 ABIN2506723 ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
0.000999302 ABIN4360942 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.000999302 ABIN4360941 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.000999302 ABIN4360943 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.000999302 ABIN4360935 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8
0.000999302 ABIN4360936 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8
0.000999302 ABIN4360944 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM Log in to see TNF706-P-T2
0.000999302 ABIN4360957 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see SPM543
0.000999302 ABIN4360940 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see P-T2
0.000999302 ABIN4360971 ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.000999302 ABIN4360958 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see SPM543
0.000999302 ABIN4360972 ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.000999302 ABIN4360977 FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM543
0.000999302 ABIN4360979 FACS ICC IF IHC (fro) IHC (p) IP Allophycocyanin Mouse IgG1 kappa Log in to see SPM543
0.000999302 ABIN4360981 FACS ICC IF IHC (fro) IHC (p) IP DyLight 650 Mouse IgG1 kappa Log in to see SPM543
0.000999302 ABIN4360982 FACS ICC IF IHC (fro) IHC (p) IP DyLight 680 Mouse IgG1 kappa Log in to see SPM543
0.000999302 ABIN4360983 FACS ICC IF IHC (fro) IHC (p) IP DyLight 488 Mouse IgG1 kappa Log in to see SPM543
0.000999302 ABIN4360989 FACS ICC IF IHC (fro) IHC (p) IP DyLight 350 Mouse IgG1 kappa Log in to see SPM543

Top referenced anti-Tumor Necrosis Factor antibody for Immunocytochemistry

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 70 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 2 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 143 Antibodies
Functional Studies (Func) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 68 Antibodies
Immunofluorescence (IF) 102 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 65 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 80-235
(35), (30), (28), (25), (24), (23), (18), (17), (13), (12), (10), (10), (10), (7), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(1243), (466), (346), (144), (137), (123), (123), (56), (56), (45), (31), (18), (8), (8), (7), (7), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(959), (479), (213), (43), (13), (9), (9), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (99), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(881), (803), (582), (258), (255), (167), (146), (146), (136), (124), (104), (63), (25), (25), (24), (22), (22), (19), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1078627) Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- L RSSSQNSSDK PVAHVVANHQ AEEQLEWLSQ RANALLANGM DLKDNQLVVP ADGLYLIYSQ VLFKGQGCPD YVLLTHTVSR FAISYQEKVS LLSAIKSPCP KDTPEGAELK PWYEPMYLGG VFQLEKGDLL SAEVNLPKYL DITESGQVYF GVIAL
Specificity It has been selected for its ability to recognize TNFa in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen Recombinant TNFa expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against TNFa.
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1078627) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor Alpha (TNFa) (TNF Antibody Abstract)
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1078627) Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant TNFa (Leu80~Leu235) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN1078627) Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

References for anti-Tumor Necrosis Factor Antibody (ABIN1078627)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Chu, Zheng, Li, Zhang, Zhang, Xu, Chen: "Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-? and NF-?B." in: Experimental and therapeutic medicine, Vol. 5, Issue 2, pp. 423-428, 2013

Wang, Zheng, Liang, Zhao, Qian, Liang, Feng: "Hepatocellular necrosis, apoptosis, and proliferation after transcatheter arterial embolization or chemoembolization in a standardized rabbit model." in: Journal of vascular and interventional radiology : JVIR, Vol. 22, Issue 11, pp. 1606-12, 2011

Volentine, Ogden, Kortje, Tuma, Sorrell: "Role of acetaldehyde in the ethanol-induced impairment of hepatic glycoprotein secretion in the rat in vivo." in: Hepatology (Baltimore, Md.), Vol. 7, Issue 3, pp. 490-5, 1987

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1078627) back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 80-235) (ABIN1078627) anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody