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anti-Rat (Rattus) Tumor Necrosis Factor antibody for Immunohistochemistry (Frozen Sections)

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Mouse (Murine), Rat (Rattus)
1229
445
338
139
132
118
118
55
55
31
26
18
8
8
7
7
4
4
4
4
2
2
1
1
1
1
Host
Rat
930
491
200
44
13
9
7
6
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
168
98
69
49
32
30
27
26
25
25
25
25
25
25
24
24
24
18
13
9
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
887
809
595
298
267
229
207
186
141
128
100
62
27
26
25
22
18
14
8
8
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN119358
$ 544.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN446837 ICC IF IHC IHC (ff) IHC (fro) IHC (p) WB Rabbit AA 100-200, Internal Region Log in to see Polyclonal 11
1 ABIN3201322 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 80-235 Log in to see 1
1 ABIN4361084 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361077 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361083 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361079 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361080 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361085 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361090 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361092 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361082 ELISA ICC IF IHC (fro) IHC (p) Biotin Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361078 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361087 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361091 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4360942 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4360941 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4360943 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361006 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgM kappa Log in to see 4C6-H8
1 ABIN4361005 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgM kappa Log in to see 4C6-H8
1 ABIN4360978 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM543

Top referenced anti-Tumor Necrosis Factor antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 18 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 119 Antibodies
ELISpot 2 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 187 Antibodies
Functional Studies (Func) 7 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 114 Antibodies
Immunofluorescence (IF) 149 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 57 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(1229), (445), (338), (139), (132), (118), (118), (55), (55), (31), (26), (18), (8), (8), (7), (7), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1)
Host Rat
(930), (491), (200), (44), (13), (9), (7), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(168), (98), (69), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (18), (13), (9), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(887), (809), (595), (298), (267), (229), (207), (186), (141), (128), (100), (62), (27), (26), (25), (22), (18), (14), (8), (8), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN119358) Images
Purification Affinity Chromatography on Protein G
Endotoxin Level less than 0.01 EU/μg
Immunogen Recombinant Murine TNF alpha expressed in E. coli.
Clone MP6-XT22
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN119358) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)
Background TNF alpha is a 17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immuno-regulatory role.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 10090
UniProt P06804
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN119358) Images back to top
Application Notes Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. ELISA: 1 μg/mL - 10 μg/mL. Functional Assays. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN119358) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 without preservatives., None, None
Preservative Without preservative
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody at 2 - 8 °C. DO NOT FREEZE!

References for anti-Tumor Necrosis Factor Antibody (ABIN119358)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Srinivas, Lovell, Skipper, Bennington, Kurban, Smith: "Ammonia absorption in calcium graphite intercalation compound: in situ neutron diffraction, Raman spectroscopy and magnetization." in: Physical chemistry chemical physics : PCCP, Vol. 12, Issue 23, pp. 6253-9, 2010

Ruan, Kang, Pei, Le: "Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease." in: Current Alzheimer research, Vol. 6, Issue 6, pp. 531-40, 2009

Hostager, Bishop: "Role of TNF receptor-associated factor 2 in the activation of IgM secretion by CD40 and CD120b." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 7, pp. 3318-22, 2002

Scanga, Mohan, Joseph, Yu, Chan, Flynn: "Reactivation of latent tuberculosis: variations on the Cornell murine model." in: Infection and immunity, Vol. 67, Issue 9, pp. 4531-8, 1999

Ross, Williams, Mason, Mauri, Marinova-Mutafchieva, Malfait, Maini, Feldmann: "Suppression of TNF-alpha expression, inhibition of Th1 activity, and amelioration of collagen-induced arthritis by rolipram." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 12, pp. 6253-9, 1998

Yang, Kawamura, Zhu, Mitsuyama: "Involvement of natural killer cells in nitric oxide production by spleen cells after stimulation with Mycobacterium bovis BCG. Study of the mechanism of the different abilities of viable and killed BCG." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 12, pp. 5728-35, 1996

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992