anti-Rhesus Monkey Tumor Necrosis Factor antibody for Western Blotting

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Epitope
AA 77-233
43
39
29
27
24
22
17
17
15
11
11
10
10
8
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Rhesus Monkey
1230
445
358
145
136
119
119
62
55
41
29
18
13
12
9
6
6
4
4
4
4
2
1
1
1
1
Host
Rabbit
929
489
182
48
10
9
7
6
3
2
2
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
165
97
71
50
32
30
27
26
25
25
25
25
25
25
24
24
24
20
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
870
852
600
311
272
230
202
180
137
117
98
70
27
26
25
22
20
16
14
8
7
6
6
6
6
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN1078629
$ 406.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.055029 ABIN3201324 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 77-233 Log in to see
1 ABIN1109408 IHC (fro) IHC (p) WB FITC Mouse IgG1 Log in to see 52B83 5
1 ABIN1109397 IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 52B83 5
1 ABIN1172411 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN1172412 ICC IF IHC WB FITC Rabbit IgG Log in to see Polyclonal

Top referenced anti-Tumor Necrosis Factor antibody for Western Blotting

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rhesus Monkey
ELISA 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 6 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 77-233
(43), (39), (29), (27), (24), (22), (17), (17), (15), (11), (11), (10), (10), (8), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rhesus Monkey
(1230), (445), (358), (145), (136), (119), (119), (62), (55), (41), (29), (18), (13), (12), (9), (6), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Rabbit
(929), (489), (182), (48), (10), (9), (7), (6), (3), (2), (2)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(165), (97), (71), (50), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (20), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(870), (852), (600), (311), (272), (230), (202), (180), (137), (117), (98), (70), (27), (26), (25), (22), (20), (16), (14), (8), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Specificity The antibody is a rabbit polyclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Immunogen TNFa (Val77-Leu233)
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name Tumor Necrosis Factor Alpha (TNFa) (TNF Antibody Abstract)
Background Alternative Names: DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes
  • Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Concentration 200 μg/mL
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Necrosis Factor antibody (TNF) (AA 77-233) (ABIN1078629) anti-Tumor Necrosis Factor (TNF) (AA 77-233) antibody