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Rat (Rattus) Tumor Necrosis Factor ELISA Kit

Recommended Tumor Necrosis Factor Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) ELISA Kits
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
86
66
53
23
13
11
11
10
9
9
9
7
5
3
2
2
1
1
Method Type
Sandwich ELISA
Detection Range
25-20000 pg/mL
Minimum Detection Limit
25 pg/mL
Application
ELISA
Options
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Catalog No. ABIN625216
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
11.926027 ABIN2506971 Sandwich ELISA Serum, Plasma 81.9-20000 pg/mL 81.9 pg/mL Log in to see
11.926027 ABIN365380 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 6.25-400 pg/mL 6.25 pg/mL Log in to see 19
11.926027 ABIN416259 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1.000 pg/mL 15.6 pg/mL Log in to see 81
11.926027 ABIN1672752 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) 15.6-1000 pg/mL 15.6 pg/mL Log in to see 36
11.926027 ABIN2472112 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 78.13-5000 pg/mL 78.13 pg/mL Log in to see
11.926027 ABIN491830 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see 2
11.926027 ABIN625217 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see 5
11.926027 ABIN1117654 Sandwich ELISA Serum, Plasma, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Log in to see 3
11.926027 ABIN924844 Sandwich ELISA Cell Lysate, Serum, Plasma 47-3000 pg/mL 47 pg/mL Log in to see
11.926027 ABIN1888511 Sandwich ELISA Plasma, Cell Culture Cells 15.6-1000 pg/mL 15.6 pg/mL Log in to see
11.926027 ABIN2708682 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
11.926027 ABIN2708681 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
11.926027 ABIN1305313 Sandwich ELISA Log in to see 1
1 ABIN456703 Sandwich ELISA Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see 5
1 ABIN772502 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see 2
1 ABIN3032882 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 27.4-20000 pg/mL 27.4 pg/mL Log in to see
1 ABIN612778 Sandwich ELISA Cell Culture Supernatant 10 pg/mL Log in to see 9
1 ABIN1446157 Cell Culture Supernatant, Serum, Plasma, Biological Samples 20 pg/mL Log in to see 4
1 ABIN1572789 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN1446554 Cell Culture Supernatant, Serum Log in to see 5

Top referenced Tumor Necrosis Factor ELISA Kit for ELISA

Similar Tumor Necrosis Factor ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 49 ELISA Kits

General

Antigen Tumor Necrosis Factor (TNF) ELISA Kits
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(86), (66), (53), (23), (13), (11), (11), (10), (9), (9), (9), (7), (5), (3), (2), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 25-20000 pg/mL
Minimum Detection Limit 25 pg/mL
Application ELISA
Pubmed 20 references available
Supplier Log in to see

Product Details Tumor Necrosis Factor ELISA Kit

Target details Application Details Handling References for Tumor Necrosis Factor Kit (ABIN625216) Images
Purpose Rat TNF alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, VEGF.
Cross-Reactivity (Details) This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1alpha, IL- 1beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3alpha, beta- NGF, TIMP-1, VEGF).
Sensitivity < 25 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details Tumor Necrosis Factor ELISA Kit Application Details Handling References for Tumor Necrosis Factor Kit (ABIN625216) Images back to top
Antigen
Alternative Name TNF-alpha (TNF ELISA Kit Abstract)
Background Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a)
Gene ID 103694380, 24835
UniProt P16599
Research Area Virology, Atherosclerosis, Immunology, Receptors, Cytokines, Growth Factors, Innate Immunity, Cancer, Cardiovascular, Inflammation
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details Tumor Necrosis Factor ELISA Kit Target details Handling References for Tumor Necrosis Factor Kit (ABIN625216) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should beused for dilution of culture supernatants and urine. Suggested dilution for normal serum/plasma: 2 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium and urine) into Item C vial to prepare a 100 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 100 µL TNF-alpha standard from the vial of Item C, into a tube with 400 µL Assay Diluent A or 1x Assay Diluent B to prepare a 20,000 pg/mL stock standard solution. Pipette 400 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 200 µL 100 µL standard + 400 µL 200myl 200 µL 200 µL 200 µL 20,000 6,667 2,222 740.7 246.9 82.30 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 200-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 50 µL of HRP-Streptavidin concentrate into a tube with 10 ml 1x Assay Diluent B to prepare a 200-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Rat TNF-alpha concentration (pg/mL) O D =4 50 n m 0.01 0.1 1 10 10 100 1,000 10,000 100,000 Assay Diluent B Rat TNF-alpha concentration (pg/mL) O D =4 50 n m 0.1 1 10 10 100 1,000 10,000 100,000
Sensitivity: The minimum detectable dose of TNF-alpha is typically less than 25 pg/mL.
Recovery: Recovery was determined by spiking various levels of Rat TNF-alpha into Rat serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 95.43 84-104 Plasma 93.79 82-102 Cell culture media 101.54 89-108
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 93 94 98 Range ( %) 82-102 84-103 88-106 1:4 Average % of Expected 96 95 101 Range ( %) 85-104 83-103 90-108
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details Tumor Necrosis Factor ELISA Kit Target details Application Details References for Tumor Necrosis Factor Kit (ABIN625216) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for Tumor Necrosis Factor Kit (ABIN625216)

Product Details Tumor Necrosis Factor ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Xu, Yang, Sun, Chen, Jiang, Zheng, Ding, Liu, Sheng, Cui, Duan: "2,3',4,4',5-Pentachlorobiphenyl Induces Inflammatory Responses in the Thyroid Through JNK and Aryl Hydrocarbon Receptor-Mediated Pathway." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 149, Issue 2, pp. 300-11, 2016

Rana, Sharma et al.: "Mechanism of sphingosine-1-phosphate induced cardioprotection against I/R injury in diabetic rat heart: Possible involvement of glycogen synthase kinase 3β and mitochondrial permeability transition ..." in: Clinical and experimental pharmacology & physiology, Vol. 43, Issue 2, pp. 166-73, 2016

Begum, Sharma, Pillai, Aeri, Sheliya: "Inhibitory effect of Careya arborea on inflammatory biomarkers in carrageenan-induced inflammation." in: Pharmaceutical biology, Vol. 53, Issue 3, pp. 437-45, 2015

Abdelkader, Safar, Salem: "Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson's Disease: Modulation of Mitochondrial Perturbations." in: Molecular neurobiology, 2015

Sharma, Deshmukh: "Vinpocetine attenuates MPTP-induced motor deficit and biochemical abnormalities in Wistar rats." in: Neuroscience, Vol. 286, pp. 393-403, 2015

Khan, Jamwal, Bijjem, Prakash, Kumar: "Neuroprotective effect of hemeoxygenase-1/glycogen synthase kinase-3? modulators in 3-nitropropionic acid-induced neurotoxicity in rats." in: Neuroscience, Vol. 287C, pp. 66-77, 2015

Zhang, Zhang, Tsui: "Mesenteric lymph duct drainage attenuates acute lung injury in rats with severe intraperitoneal infection." in: Inflammation, Vol. 38, Issue 3, pp. 1239-49, 2015

Kabel, Abd Elmaaboud, Albarraq: "Ameliorative potential of omega 3 fatty acids and HMG-CoA reductase inhibitors on experimentally-induced non-alcoholic steatohepatitis." in: Prostaglandins, leukotrienes, and essential fatty acids, Vol. 96, pp. 1-9, 2015

Elshazly: "Ameliorative effect of nicorandil on high fat diet induced non-alcoholic fatty liver disease in rats." in: European journal of pharmacology, Vol. 748, pp. 123-32, 2015

Tyagi, Bisht, Pant, Kumar, Majeed, Prakash: "Possible role of GABA-B receptor modulation in MPTP induced Parkinson's disease in rats." in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, Vol. 67, Issue 2, pp. 211-7, 2015

Yaidikar, Thakur: "Punicalagin attenuated cerebral ischemia-reperfusion insult via inhibition of proinflammatory cytokines, up-regulation of Bcl-2, down-regulation of Bax, and caspase-3." in: Molecular and cellular biochemistry, Vol. 402, Issue 1-2, pp. 141-8, 2015

Aral, Kesim, Greenwell, Kara, Çetin, Yakan: "Alveolar bone protective and hypoglycemic effects of systemic propolis treatment in experimental periodontitis and diabetes mellitus." in: Journal of medicinal food, Vol. 18, Issue 2, pp. 195-201, 2015

Nassar, Al-Shorbagy, Arab, Abdallah: "Saxagliptin: a novel antiparkinsonian approach." in: Neuropharmacology, Vol. 89, pp. 308-17, 2015

Sayd, Antón, Alén, Caso, Pavón, Leza, Rodríguez de Fonseca, García-Bueno, Orio: "Systemic administration of oleoylethanolamide protects from neuroinflammation and anhedonia induced by LPS in rats." in: The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP), Vol. 18, Issue 6, 2015

Serya, Abbas, Ismail, Esmat, Abou El Ella: "Design, synthesis and biological evaluation of novel quinazoline-based anti-inflammatory agents acting as PDE4B inhibitors." in: Chemical & pharmaceutical bulletin, Vol. 63, Issue 2, pp. 102-16, 2015

Abdel Kawy: "Cilostazol attenuates cholestatic liver injury and its complications in common bile duct ligated rats." in: European journal of pharmacology, Vol. 752, pp. 8-17, 2015

Choudhary, Sheela Devi: "Longer period of oral administration of aspartame on cytokine response in Wistar albino rats." in: Endocrinologi?a y nutricio?n : o?rgano de la Sociedad Espan?ola de Endocrinologi?a y Nutricio?n, Vol. 62, Issue 3, pp. 114-22, 2015

Chen, Min, Wang, Leung, Shi, Zhou, Yu, Wang, An, Sha, Chen: "Pre-activation of mesenchymal stem cells with TNF-?, IL-1? and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury." in: Scientific reports, Vol. 5, pp. 8718, 2015

Abdelsalam, Safar: "Neuroprotective effects of vildagliptin in rat rotenone Parkinson's disease model: role of RAGE-NF?B and Nrf2-antioxidant signaling pathways." in: Journal of neurochemistry, Vol. 133, Issue 5, pp. 700-7, 2015

Shete, Pawar, Thanki, Jain, Bansal: "Oral bioavailability and pharmacodynamic activity of hesperetin nanocrystals generated using a novel bottom-up technology." in: Molecular pharmaceutics, Vol. 12, Issue 4, pp. 1158-70, 2015

Images

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Supplier Images
ELISA image for Tumor Necrosis Factor ELISA Kit (TNF) (ABIN625216) Tumor Necrosis Factor (TNF) ELISA Kit