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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)||Horse (Equine)||Monkey||Rabbit|
|Western Blotting (WB)||149 Antibodies||72 Antibodies||56 Antibodies||2 Antibodies||2 Antibodies||4 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||29 Antibodies||16 Antibodies||9 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||53 Antibodies||27 Antibodies||24 Antibodies|
|Immunohistochemistry (IHC)||71 Antibodies||35 Antibodies||34 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||9 Antibodies|
|Antigen||Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies|
|Reactivity||Cow (Bovine), Horse (Equine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus) Alternatives|
|Conjugate||This APAF1 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-APAF1 AntibodyTarget Details APAF1 Application Details Handling References for anti-APAF1 Antibody (ABIN222876) Images
|Specificity||The immunoaffinity purified antibody detects a 130 kDa protein, corresponding to the apparent molecular weight of Apaf-1 on SDS-PAGE immunoblots.|
|Immunogen||Synthetic peptide surrounding amino acid 1161 of human Apaf-1|
Target Details APAF1Product Details anti-APAF1 Antibody Application Details Handling References for anti-APAF1 Antibody (ABIN222876) Images back to top
|Alternative Name||Apaf-1 (APAF1 Antibody Abstract)|
During apoptosis, Apaf-1 (apoptosis protease activating factor 1) binds to cytochrome c (Apaf-2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase-9, and activates caspase-9 (Apaf-3). Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 is ubiquitously expressed in human tissues.
Synonyms: APAF1, CED4, DKFZp781B1145, KIAA0413
|Pathways||p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-APAF1 Antibody Target Details APAF1 Handling References for anti-APAF1 Antibody (ABIN222876) Images back to top
Western blotting (0.5-4 μg/mL).
However, the optimal conditions should be determined individually.
Country of Animal Origin: China
|Restrictions||For Research Use only|
HandlingProduct Details anti-APAF1 Antibody Target Details APAF1 Application Details References for anti-APAF1 Antibody (ABIN222876) Images back to top
|Buffer||100 μg (0.2 mg/mL) immunoaffinity purified, rabbit anti-Apaf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.05 % BSA, 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-APAF1 Antibody (ABIN222876)Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling Images back to top
|Product cited in:||
Morikawa, Nagahiro, Endo, Sato, Nakashima, Takatani, Mitsumori: "[Intralobar sequestration in elderly patient, report of a case]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 9, pp. 812-5, 2008
Siu, Alway: "Mitochondria-associated apoptotic signalling in denervated rat skeletal muscle." in: The Journal of physiology, Vol. 565, Issue Pt 1, pp. 309-23, 2005
Dirks, Leeuwenburgh: "Apoptosis in skeletal muscle with aging." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 282, Issue 2, pp. R519-27, 2002
Phaneuf, Leeuwenburgh: "Cytochrome c release from mitochondria in the aging heart: a possible mechanism for apoptosis with age." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 282, Issue 2, pp. R423-30, 2002
ImagesProduct Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling References for anti-APAF1 Antibody (ABIN222876) back to top