anti-Human APAF1 antibody for Enzyme Immunoassay

Recommended APAF1 Antibody (supplied by: Log in to see )

Antigen
Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
  • APAF1
  • ARK
  • Apaf-1
  • Apaf1
  • CG6829
  • D-Apaf-1
  • DARK
  • Dapaf-1
  • Dapaf-1/HAC-1
  • Dark
  • Dark/Apaf-I
  • Dark/Dapaf-1/HAC1
  • Dark/Hac-1/dApaf-1
  • Dark/Hac-1/dApaf1
  • Dmel\\CG6829
  • Hac-1
  • Hac-1/Dark
  • Hac1
  • T1
  • anon-53Fa
  • apaf-1
  • apaf1
  • arc
  • ark
  • dAPAF-1
  • dApaf-1
  • dApaf-1/DARK/HAC-1
  • dApaf1
  • dArk
  • dapaf
  • dapaf-1
  • dapaf-1L
  • dapaf-1S
  • dark
  • dark/dapaf-1/hac-1
  • dark/hac-1/dapaf-1
  • hac-1
  • hac1
  • l(2)SH0173
  • APAF-1
  • CED4
  • 6230400I06Rik
  • Apaf1l
  • fog
  • mKIAA0413
  • zgc:136543
  • Apaf-1-related-killer
  • apoptotic peptidase activating factor 1
  • apoptotic protease activating factor 1
  • Ark
  • apaf1
  • APAF1
  • Apaf1
Reactivity
Human
200
129
110
7
4
3
3
3
2
1
Host
Rabbit
170
25
13
3
Clonality
Polyclonal
Conjugate
This APAF1 antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
163
72
62
59
36
23
22
16
7
4
3
3
2
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Catalog No. ABIN265312
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5554539 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5554538 EIA IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN5551027 EIA IHC (p) Mouse IgG C-Term Log in to see 5-00E-011

General

Antigen Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
Reactivity Human
(200), (129), (110), (7), (4), (3), (3), (3), (2), (1)
Host Rabbit
(170), (25), (13), (3)
Clonality
Polyclonal
Conjugate This APAF1 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(163), (72), (62), (59), (36), (23), (22), (16), (7), (4), (3), (3), (2)
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Product Details anti-APAF1 Antibody

Target Details APAF1 Application Details Handling Images
Specificity This antibody detects endogenous levels of APAF1 protein (region surrounding His324).
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE

Target Details APAF1

Product Details anti-APAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name APAF1 (APAF1 Antibody Abstract)
Background Studies of apoptosis using the nematode Caenorhabditis elegans as a model system have identified three genes, ced-3, ced-4 and ced-9, as core components in cell death. The Ced-4 protein functions downstream of the apoptotic inhibitor Ced-9 and upstream of Ced-3 to promote cell death. Direct interactions of Ced-4 with Ced-9 and Ced-3 suggest a central role for this protein in the regulation of apoptosis. The mammalian protein AFAP1 contains a domain with homology to Ced-4 and may function in a similar manner by mediating apoptosis downstream of the survival protein Bcl-2 and upstream of the apoptotic inducing caspases. AFAP1 has been shown to bind to cytosolic cytochrome c, and it may mediate the activation of caspase-3 by cytochrome c.Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413
Molecular Weight approx. 38 kDa
Gene ID 317
NCBI Accession NP_001151
UniProt O14727
Research Area Cancer, Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-APAF1 Antibody Target Details APAF1 Handling Images back to top
Application Notes ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling back to top
Supplier Images
 image for anti-Apoptotic Peptidase Activating Factor 1 (APAF1) antibody (ABIN265312) anti-Apoptotic Peptidase Activating Factor 1 (APAF1) antibody
 image for anti-Apoptotic Peptidase Activating Factor 1 (APAF1) antibody (ABIN265312) anti-Apoptotic Peptidase Activating Factor 1 (APAF1) antibody (Image 2)