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anti-Human APAF1 antibody for Immunocytochemistry

Recommended APAF1 Antibody (supplied by: Log in to see )

Antigen
Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
  • 6230400I06Rik
  • anon-53Fa
  • apaf-1
  • Apaf-1
  • APAF-1
  • Apaf1
  • apaf1
  • APAF1
  • Apaf1l
  • arc
  • ARK
  • ark
  • CED4
  • CG6829
  • D-Apaf-1
  • dapaf
  • Dapaf-1
  • dAPAF-1
  • dapaf-1
  • dApaf-1
  • dApaf-1/DARK/HAC-1
  • Dapaf-1/HAC-1
  • dapaf-1L
  • dapaf-1S
  • dApaf1
  • DARK
  • dark
  • dArk
  • Dark
  • Dark/Apaf-I
  • dark/dapaf-1/hac-1
  • Dark/Dapaf-1/HAC1
  • Dark/Hac-1/dApaf-1
  • dark/hac-1/dapaf-1
  • Dark/Hac-1/dApaf1
  • Dmel\\CG6829
  • fog
  • hac-1
  • Hac-1
  • Hac-1/Dark
  • hac1
  • Hac1
  • l(2)SH0173
  • mKIAA0413
  • T1
  • zgc:136543
Epitope
AA 1-464, N-Term
46
43
17
13
13
8
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
188
104
85
4
2
2
2
1
1
Host
Rat
163
23
12
3
Clonality
Monoclonal
Conjugate
This APAF1 antibody is un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
147
59
58
42
28
22
14
9
8
5
4
Supplier
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Catalog No. ABIN2745508
$ 351.75
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010160999 ABIN2745510 ICC IP ELISA WB Rat IgG2a AA 1-97, N-Term Log in to see 18H2
0.0010160999 ABIN204434 ICC IHC (fro) IP ELISA WB Rat IgG2a AA 1-464 Log in to see
0.0010160999 ABIN1866706 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 31-350 Log in to see Polyclonal
0.0010160999 ABIN1731833 ICC IF IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010160999 ABIN1731834 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.0010160999 ABIN2961887 ICC IP IHC (p) ELISA WB Rat IgG2a kappa AA 1-464 Log in to see 2E12
0.0010160999 ABIN326858 ICC IHC WB Chicken IgY AA 307-388 Log in to see Polyclonal
0.0010160999 ABIN2616748 ICC Rabbit C-Term Log in to see Polyclonal

Similar anti-APAF1 Antibodies

Application / Reactivity Human
ELISA 52 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 58 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Western Blotting (WB) 140 Antibodies

General

Antigen Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
Epitope AA 1-464, N-Term
(46), (43), (17), (13), (13), (8), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(188), (104), (85), (4), (2), (2), (2), (1), (1)
Host Rat
(163), (23), (12), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This APAF1 antibody is un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(147), (59), (58), (42), (28), (22), (14), (9), (8), (5), (4)
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Product Details anti-APAF1 Antibody

Target Details APAF1 Application Details Handling Images
Specificity Recognizes the CARD domain of human Apaf-1.
Cross-Reactivity Human
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) Does not cross-react with mouse or rat Apaf-1.
Purity >95 % (SDS-PAGE)
Endotoxin Level <0.1EU/μg purified protein (LAL test, Lonza).
Immunogen Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.
Clone 2E12
Isotype IgG2a

Target Details APAF1

Product Details anti-APAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Apaf-1 (APAF1 Antibody Abstract)
Background Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
UniProt O14727
Research Area Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-APAF1 Antibody Target Details APAF1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. In PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months