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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||33 Antibodies||41 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies||4 Antibodies|
|Immunocytochemistry (ICC)||10 Antibodies||15 Antibodies|
|Immunofluorescence (IF)||6 Antibodies||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies||22 Antibodies|
|Immunohistochemistry (IHC)||40 Antibodies||43 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||33 Antibodies|
|Immunoprecipitation (IP)||14 Antibodies|
|Western Blotting (WB)||75 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Antigen||Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This APAF1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-APAF1 AntibodyTarget Details APAF1 Application Details Handling References for anti-APAF1 Antibody (ABIN400811) Images
|Specificity||This Antibody is directed against Human APAF1 protein.|
|Characteristics||Synonyms: Apaf-1, KIAA0413, Apoptotic protease-activating factor 1|
|Purification||Affinity Chromatography on Protein A|
|Immunogen||Synthetic peptide corresponding to a region near the carboxy terminus of human Apaf1protein|
Target Details APAF1Product Details anti-APAF1 Antibody Application Details Handling References for anti-APAF1 Antibody (ABIN400811) Images back to top
|Alternative Name||APAF1 (APAF1 Antibody Abstract)|
|Background||Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologue of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413|
|Research Area||Cancer, Apoptosis/Necrosis|
|Pathways||p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-APAF1 Antibody Target Details APAF1 Handling References for anti-APAF1 Antibody (ABIN400811) Images back to top
ELISA: 1/10,000 - 1/40,000. Western Blot: 1/200 - 1/2,000. IF Microscopy: 1/100 - 1/1,000. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-APAF1 Antibody Target Details APAF1 Application Details References for anti-APAF1 Antibody (ABIN400811) Images back to top
|Concentration||1.0 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.02 % (w/v) Sodium Azide, None|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-APAF1 Antibody (ABIN400811)Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling Images back to top
Cecconi, Alvarez-Bolado, Meyer, Roth, Gruss: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998
Hu, Benedict, Wu, Inohara, Núñez: "Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4386-91, 1998
Zou, Henzel, Liu, Lutschg, Wang: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997
Li, Nijhawan, Budihardjo, Srinivasula, Ahmad, Alnemri, Wang: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997
ImagesProduct Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling References for anti-APAF1 Antibody (ABIN400811) back to top