anti-Rat (Rattus) APAF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended APAF1 Antibody (supplied by: Log in to see )

Antigen
Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
  • APAF1
  • ARK
  • Apaf-1
  • Apaf1
  • CG6829
  • D-Apaf-1
  • DARK
  • Dapaf-1
  • Dapaf-1/HAC-1
  • Dark
  • Dark/Apaf-I
  • Dark/Dapaf-1/HAC1
  • Dark/Hac-1/dApaf-1
  • Dark/Hac-1/dApaf1
  • Dmel\\CG6829
  • Hac-1
  • Hac-1/Dark
  • Hac1
  • T1
  • anon-53Fa
  • apaf-1
  • apaf1
  • arc
  • ark
  • dAPAF-1
  • dApaf-1
  • dApaf-1/DARK/HAC-1
  • dApaf1
  • dArk
  • dapaf
  • dapaf-1
  • dapaf-1L
  • dapaf-1S
  • dark
  • dark/dapaf-1/hac-1
  • dark/hac-1/dapaf-1
  • hac-1
  • hac1
  • l(2)SH0173
  • APAF-1
  • CED4
  • 6230400I06Rik
  • Apaf1l
  • fog
  • mKIAA0413
  • zgc:136543
  • Apaf-1-related-killer
  • apoptotic peptidase activating factor 1
  • apoptotic protease activating factor 1
  • Ark
  • apaf1
  • APAF1
  • Apaf1
Epitope
N-Term
43
41
17
13
13
8
5
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
194
128
109
7
4
3
3
3
2
1
Host
Rabbit
165
24
13
3
Clonality
Polyclonal
Conjugate
This APAF1 antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
159
71
60
57
36
22
21
17
8
5
4
3
Supplier
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Catalog No. ABIN1105393
$ 313.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.403178 ABIN4280961 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 2
13.403178 ABIN4280963 IHC IHC (p) Rabbit IgG C-Term Log in to see Polyclonal
13.403178 ABIN214605 IHC (p) WB Rabbit AA 12-28 Log in to see Polyclonal
13.403178 ABIN615100 IHC (p) Rabbit C-Term Log in to see Polyclonal
13.403178 ABIN4280960 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal
13.403178 ABIN4280957 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN3044356 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN252079 ICC IHC (fro) IF IHC (p) IP IHC WB Rabbit Log in to see Polyclonal 1
1 ABIN3187886 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN724295 IF (p) IHC (p) WB Rabbit IgG AA 1200-1248, C-Term Log in to see Polyclonal
1 ABIN724310 IF (p) IHC (p) WB Rabbit IgG AA 3-44, N-Term Log in to see Polyclonal
1 ABIN303381 IHC (p) WB Rabbit AA 12-28 Log in to see Polyclonal
1 ABIN5554538 EIA IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN5554539 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1495140 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN724304 IHC (p) WB HRP Rabbit IgG AA 1200-1248, C-Term Log in to see Polyclonal
1 ABIN724319 IHC (p) WB HRP Rabbit IgG AA 3-44, N-Term Log in to see Polyclonal
1 ABIN957135 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN1804017 IHC (p) Rabbit C-Term Log in to see Polyclonal
1 ABIN1731835 IHC (p) WB Rabbit IgG AA 12-28 Log in to see Polyclonal

Top referenced anti-APAF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-APAF1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies
Western Blotting (WB) 149 Antibodies 91 Antibodies 72 Antibodies
Immunoprecipitation (IP) 30 Antibodies 20 Antibodies 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 58 Antibodies 36 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 68 Antibodies 39 Antibodies 36 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies 4 Antibodies 4 Antibodies

General

Antigen Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
Epitope N-Term
(43), (41), (17), (13), (13), (8), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(194), (128), (109), (7), (4), (3), (3), (3), (2), (1)
Host Rabbit
(165), (24), (13), (3)
Clonality
Polyclonal
Conjugate This APAF1 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(159), (71), (60), (57), (36), (22), (21), (17), (8), (5), (4), (3)
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Product Details anti-APAF1 Antibody

Target Details APAF1 Application Details Handling Images
Specificity This antibody detects APAF1 (N-term). No cross reactivity with other proteins.
Cross-Reactivity (Details) Species reactivity (tested):Human, rat, mouse.
Purification Immunogen affinity purified
Immunogen A synthetic peptide corresponding to a sequence at the N-terminal of human APAF1 .Remarks: Sequence is identical to the related rat and mouse sequence.
Isotype IgG

Target Details APAF1

Product Details anti-APAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name APAF1 (APAF1 Antibody Abstract)
Background Apoptotic peptidase activating factor 1, also known as APAF1, is a protein which in humans is encoded by the APAF1 gene. This gene is mapped to chromosome 12q23.1 It encodes a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis.2 This protein contains several copies of the WD40 repeat domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). In the presence of cytochrome c and dATP, APAF1 assembles into an oligomeric apoptosome, which is responsible for activation of procaspase-9 and maintenance of the enzymatic activity of processed caspase-9.3 Furthermore, APAF1 is inactivated in metastatic melanomas, leading to defects in the execution of apoptotic cell death.4 Additionally, APAF1 has been shown to interact with NLRP1,5 Caspase-9,5, 6, 7, 8, 9, 10 APIP,6 BCL2-like 18, 9 and HSPA4.10Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413
Gene ID 317
NCBI Accession NP_001151
UniProt O14727
Research Area Cancer, Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-APAF1 Antibody Target Details APAF1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Images back to top
Reconstitution 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Buffer 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
Preservative Sodium azide, Thimerosal (Merthiolate)
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (N-Term) (ABIN1105393) Immunohistochemistry with APAF1 Polyclonal Antibody
Western Blotting (WB) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (N-Term) (ABIN1105393) Western blot with APAF1 Polyclonal Antibody