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anti-Rat (Rattus) APAF1 antibody for Western Blotting

Recommended APAF1 Antibody (supplied by: Log in to see )

Antigen
Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
  • 6230400I06Rik
  • anon-53Fa
  • apaf-1
  • Apaf-1
  • APAF-1
  • Apaf1
  • apaf1
  • APAF1
  • Apaf1l
  • arc
  • ARK
  • ark
  • CED4
  • CG6829
  • D-Apaf-1
  • dapaf
  • Dapaf-1
  • dAPAF-1
  • dapaf-1
  • dApaf-1
  • dApaf-1/DARK/HAC-1
  • Dapaf-1/HAC-1
  • dapaf-1L
  • dapaf-1S
  • dApaf1
  • DARK
  • dark
  • dArk
  • Dark
  • Dark/Apaf-I
  • dark/dapaf-1/hac-1
  • Dark/Dapaf-1/HAC1
  • Dark/Hac-1/dApaf-1
  • dark/hac-1/dapaf-1
  • Dark/Hac-1/dApaf1
  • Dmel\\CG6829
  • fog
  • hac-1
  • Hac-1
  • Hac-1/Dark
  • hac1
  • Hac1
  • l(2)SH0173
  • mKIAA0413
  • T1
  • zgc:136543
Epitope
C-Term
45
44
17
13
13
8
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
201
110
90
4
2
2
2
1
1
1
Host
Mouse
173
24
13
4
Clonality
Monoclonal
Conjugate
This APAF1 antibody is un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
158
71
64
52
34
22
21
12
8
4
4
Supplier
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Catalog No. ABIN400811
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN222876 WB Rabbit IgG Log in to see Polyclonal 4
0.13341247 ABIN499287 EIA IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 4
0.13341247 ABIN499289 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.13341247 ABIN1105393 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.13341247 ABIN3044356 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.13341247 ABIN1871007 WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN1871011 WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2705478 WB Rabbit Center Log in to see Polyclonal
0.13341247 ABIN3187886 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.13341247 ABIN2155902 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN306747 WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.13341247 ABIN2155903 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.13341247 ABIN2155901 IHC ELISA WB Mouse C-Term Log in to see 2-00E-010
0.13341247 ABIN1031229 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.13341247 ABIN1030242 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN2430931 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2425445 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN1031715 IHC ELISA WB Mouse Log in to see 2E10
0.13341247 ABIN189873 WB Rabbit C-Term Log in to see Polyclonal
0.0011728023 ABIN264991 WB Rabbit IgG AA 12-28 Log in to see Polyclonal 1

Top referenced anti-APAF1 antibody for Western Blotting

Similar anti-APAF1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 31 Antibodies 39 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 14 Antibodies
Immunofluorescence (IF) 5 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 32 Antibodies 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies 25 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 56 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
Epitope C-Term
(45), (44), (17), (13), (13), (8), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(201), (110), (90), (4), (2), (2), (2), (1), (1), (1)
Host Mouse
(173), (24), (13), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This APAF1 antibody is un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(158), (71), (64), (52), (34), (22), (21), (12), (8), (4), (4)
Pubmed 4 references available
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Product Details anti-APAF1 Antibody

Target Details APAF1 Application Details Handling References for anti-APAF1 Antibody (ABIN400811) Images
Specificity This Antibody is directed against Human APAF1 protein.
Characteristics Synonyms: Apaf-1, KIAA0413, Apoptotic protease-activating factor 1
Purification Affinity Chromatography on Protein A
Immunogen Synthetic peptide corresponding to a region near the carboxy terminus of human Apaf1protein
Clone 2-00E-010
Isotype IgG

Target Details APAF1

Product Details anti-APAF1 Antibody Application Details Handling References for anti-APAF1 Antibody (ABIN400811) Images back to top
Antigen
Alternative Name APAF1 (APAF1 Antibody Abstract)
Background Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologue of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413
Gene ID 317
UniProt O14727
Research Area Cancer, Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-APAF1 Antibody Target Details APAF1 Handling References for anti-APAF1 Antibody (ABIN400811) Images back to top
Application Notes ELISA: 1/10,000 - 1/40,000. Western Blot: 1/200 - 1/2,000. IF Microscopy: 1/100 - 1/1,000. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details References for anti-APAF1 Antibody (ABIN400811) Images back to top
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.02 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-APAF1 Antibody (ABIN400811)

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling Images back to top
Background publications

Cecconi, Alvarez-Bolado, Meyer, Roth, Gruss: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998

Hu, Benedict, Wu, Inohara, Núñez: "Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4386-91, 1998

Zou, Henzel, Liu, Lutschg, Wang: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997

Li, Nijhawan, Budihardjo, Srinivasula, Ahmad, Alnemri, Wang: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997

Images

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling References for anti-APAF1 Antibody (ABIN400811) back to top
Supplier Images
Western Blotting (WB) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (C-Term) (ABIN400811) anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (C-Term) antibody
Immunohistochemistry (IHC) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (C-Term) (ABIN400811) anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (C-Term) antibody (Image 2)