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anti-Human ARFGAP1 antibody for Western Blotting

Recommended ARFGAP1 Antibody (supplied by: Log in to see )

Antigen
ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) Antibodies
  • AI115377
  • arf1gap
  • ARF1GAP
  • Arf1gap
  • Arf GAP1
  • ARFGAP3
  • atfb1
  • atfb3
  • HRIHFB2281
  • jlns1
  • k(v)7.1
  • kcna8
  • kcna9
  • kv1.9
  • kv7.1
  • kvlqt1
  • lqt
  • lqt1
  • rws
  • sqt2
  • wrs
Epitope
AA 377-406, C-Term
18
15
4
3
2
2
2
1
1
1
Reactivity
Human, Rat (Rattus)
73
16
14
6
Host
Rabbit
51
22
1
Clonality
Polyclonal
Conjugate
This ARFGAP1 antibody is un-conjugated
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
68
33
33
29
21
4
2
2
1
1
Supplier
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Catalog No. ABIN388996
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12227175 ABIN1534608 IF ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12227175 ABIN2787085 WB Rabbit Middle Region Log in to see Polyclonal
0.12227175 ABIN2787084 WB Rabbit Middle Region Log in to see Polyclonal
0.12227175 ABIN2454350 FACS IF WB Mouse IgG2b Log in to see 1F9
0.12227175 ABIN2454349 FACS IF WB Mouse IgG2b Log in to see 2C6
0.12227175 ABIN2454382 FACS IF WB Mouse IgG2b Log in to see 2C5
0.12227175 ABIN2454308 FACS IF WB Mouse IgG1 Log in to see 1H6
0.12227175 ABIN2454352 FACS IF IHC WB Mouse IgG2b Log in to see 1H4
0.12227175 ABIN2454351 FACS IF IHC WB Mouse IgG2b Log in to see 1F6
0.12227175 ABIN2454386 FACS IF WB Mouse IgG2b Log in to see 2C10
0.12227175 ABIN2454434 FACS IF WB Mouse IgG2b Log in to see 2D5
0.12227175 ABIN438754 IP WB Rabbit IgG AA 356-406 Log in to see Polyclonal
0.12227175 ABIN2162683 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.12227175 ABIN2159221 IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.12227175 ABIN928933 WB Rabbit Middle Region Log in to see Polyclonal
0.12227175 ABIN928934 WB Rabbit Middle Region Log in to see Polyclonal
0.12227175 ABIN2422639 ELISA WB Rabbit IgG Log in to see Polyclonal
0.12227175 ABIN2429235 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011833628 ABIN357645 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 1
0.0011833628 ABIN1496683 FACS IF WB Mouse IgG2b Log in to see 2C6

Top referenced anti-ARFGAP1 antibody for Western Blotting

Similar anti-ARFGAP1 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 9 Antibodies 32 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 2 Antibodies
Immunofluorescence (IF) 6 Antibodies 28 Antibodies
Immunohistochemistry (IHC) 9 Antibodies 32 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 5 Antibodies
Western Blotting (WB) 15 Antibodies 69 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Immunoprecipitation (IP) 3 Antibodies

General

Antigen ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) Antibodies
Epitope AA 377-406, C-Term
(18), (15), (4), (3), (2), (2), (2), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(73), (16), (14), (6)
Host Rabbit
(51), (22), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This ARFGAP1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(68), (33), (33), (29), (21), (4), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-ARFGAP1 Antibody

Target Details ARFGAP1 Application Details Handling References for anti-ARFGAP1 Antibody (ABIN388996) Images
Specificity This ARFGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377~406 amino acids from the C-terminal region of human ARFGAP1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This ARFGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 AA from the C-terminal region of human ARFGAP1.
Clone RB4855
Isotype Ig

Target Details ARFGAP1

Product Details anti-ARFGAP1 Antibody Application Details Handling References for anti-ARFGAP1 Antibody (ABIN388996) Images back to top
Antigen
Alternative Name ARFGAP1 (ARFGAP1 Antibody Abstract)
Background ARFGAP1 is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine.
Synonyms: ADP-ribosylation factor GTPase-activating protein 1, ADP-ribosylation factor 1 GTPase-activating protein, ARF1 GAP, ARF1-directed GTPase-activating protein, GAP protein, ARF1GAP
Molecular Weight 44668 DA
Gene ID 55738
UniProt Q8N6T3
Research Area Protein Modifications, Cell Structure
Pathways p53 Signaling

Application Details

Product Details anti-ARFGAP1 Antibody Target Details ARFGAP1 Handling References for anti-ARFGAP1 Antibody (ABIN388996) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-ARFGAP1 Antibody Target Details ARFGAP1 Application Details References for anti-ARFGAP1 Antibody (ABIN388996) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-ARFGAP1 Antibody (ABIN388996)

Product Details anti-ARFGAP1 Antibody Target Details ARFGAP1 Application Details Handling Images back to top
Product cited in:

Nishimura, Uchida, Yachi, Kudlyk, Lupashin, Inoue, Taguchi, Arai: "Oxysterol-binding protein (OSBP) is required for the perinuclear localization of intra-Golgi v-SNAREs." in: Molecular biology of the cell, Vol. 24, Issue 22, pp. 3534-44, 2013

Zhang, Major, Takanashi, Camp, Nishiya, Peters, Ginsberg, Jian, Randazzo, Schultz, Moon, Ding: "Small-molecule synergist of the Wnt/beta-catenin signaling pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 18, pp. 7444-8, 2007

Background publications

Huber, Rotman, Pick, Makler, Rothem, Cukierman, Cassel: "Expression, purification, and properties of ADP-ribosylation factor (ARF) GTPase activating protein-1." in: Methods in enzymology, Vol. 329, pp. 307-16, 2001

Images

Product Details anti-ARFGAP1 Antibody Target Details ARFGAP1 Application Details Handling References for anti-ARFGAP1 Antibody (ABIN388996) back to top
Supplier Images
Western Blotting (WB) image for anti-ARFGAP1 antibody (ADP-Ribosylation Factor GTPase Activating Protein 1) (AA 377-406) (ABIN388996) Western blot analysis of ARFGAP1 Antibody (C-term) (ABIN388996) against rat recombina...
Immunohistochemistry (IHC) image for anti-ARFGAP1 antibody (ADP-Ribosylation Factor GTPase Activating Protein 1) (AA 377-406) (ABIN388996) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...