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anti-Human ATM antibody for BioImaging

Recommended ATM Antibody (supplied by: Log in to see )

Antigen
Ataxia Telangiectasia Mutated (ATM) Antibodies
  • AI256621
  • at1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • atdc
  • ATE
  • Atm
  • ATM
  • atm
  • atm/tefu
  • C030026E19Rik
  • CG6535
  • dATM
  • Dmel\\CG6535
  • tef
  • Tefu
  • tel1
  • TEL1
  • TELO1
  • telo1
  • Xatm
Epitope
pSer1981
85
37
17
14
13
13
13
7
7
7
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
320
113
59
14
6
4
1
Host
Mouse
214
83
14
8
3
Clonality
Monoclonal
Conjugate
This ATM antibody is un-conjugated
11
10
8
8
6
6
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
BioImaging (BI), Western Blotting (WB)
196
111
99
89
77
74
53
23
22
14
11
4
4
2
2
1
1
1
Supplier
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Catalog No. ABIN2688845
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Top referenced anti-ATM antibody for BioImaging

Similar anti-ATM Antibodies

Application / Reactivity Mouse (Murine) Human
Biochemical Assay (BCA) 2 Antibodies 2 Antibodies
BioImaging (BI) 1 Antibodies 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 1 Antibodies 1 Antibodies
ELISA 29 Antibodies 99 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Immunocytochemistry (ICC) 50 Antibodies
Immunofluorescence (IF) 57 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 56 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunoprecipitation (IP) 53 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 81 Antibodies
Dot Blot (DB) 22 Antibodies

General

Antigen Ataxia Telangiectasia Mutated (ATM) Antibodies
Epitope pSer1981
(85), (37), (17), (14), (13), (13), (13), (7), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(320), (113), (59), (14), (6), (4), (1)
Host Mouse
(214), (83), (14), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ATM antibody is un-conjugated
(11), (10), (8), (8), (6), (6), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application BioImaging (BI), Western Blotting (WB)
(196), (111), (99), (89), (77), (74), (53), (23), (22), (14), (11), (4), (4), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-ATM Antibody

Target Details ATM Application Details Handling References for anti-ATM Antibody (ABIN2688845) Images
Brand BD Pharmingen™
Characteristics ATM is the gene that is mutated in ataxia telangiectasia, an autosomal recessive hereditary disease that is characterized by delayed motor and sexual development, weakened immunity, multiple skin changes, increased sensitivity to ionizing radiation, and increased risk of developing certain cancers. The protein encoded by ATM is a phophoinositide-3-kinase-related protein kinase that is found in the nucleus and is responsible for early responses to the changes in chromatin structure caused by DNA double-strand breaks. Inactive wild type ATM protein forms homodimers or higher order multimers in which the C-terminal kinase domains are inhibited. It has been proposed that multimeric ATM is recruited to DNA double-strand breaks through interactions between its N-terminal HEAT domains and specific chromatin protein complexes. The inhibition of the kinase domains is lifted, resulting in auto-phosphorylation of the ATM at a single serine site and disruption of the multimers. The phosphorylated state of ATM is a rapid and highly sensitive indicator that cells have been exposed to agents that cause DNA double-strand breaks. The kinase domains of the resulting ATM monomers are accessible to a wide variety of substrates that are involved in DNA repair, cell-cycle regulation, and apoptosis. Thus ATM is a central regulator of cellular responses to ionizing radiation, and cells that lack ATM undergo radioresistant DNA synthesis and are resistant to γ radiation-induced apoptosis. The K88-534 monoclonal antibody recognizes human ATM phosphorylated at serine 1981 (S1981) and mouse ATM phosphorylated at its orthologous site, S1987. Immunofluorescent detection of ATM phosphorylation in human fibrosarcoma cells. HT-1080 cells (ATCC CCL-121) were seeded in a BD Falcon™ 96-well Imaging Plate (Cat. No. 353219) at ~ 10,000 cells per well. After overnight culture, DNA damage was induced by exposing the cells to bleomycin sulfate (250 units/mL) in serum-free medium for 2 hours at 37 °C (left panel) or the cells were untreated (right panel). To allow time for DNA repair processes to occur, the cells were washed, replenished with fresh complete medium, and returned to the incubator for 30 minutes. After treatment, cells were stained using the alcohol perm protocol and the Purified Mouse anti-ATM (pS1981) (pseudo-colored green), and counter-stained with Hoechst 33342 (pseudo-colored blue) according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Invitrogen). Confocal images were captured on a BD Pathway™ 855 Bioimaging System using a 20x (0.75 NA) objective. Five sections, separated by 1 μm each, were captured and collapsed for viewing purposes using BD Attovision™ software. 560007 Rev. 1 Page 1 of 2

BD Pharmingen™ Purified Mouse anti-ATM (pS1981) - Purified - Clone K88-534 - Isotype Mouse IgG2b, κ - Reactivity Hu, Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Mouse ATM Peptide
Clone K88
Isotype IgG2b, kappa

Target Details ATM

Product Details anti-ATM Antibody Application Details Handling References for anti-ATM Antibody (ABIN2688845) Images back to top
Antigen
Alternative Name ATM (ATM Antibody Abstract)
Molecular Weight 349 kDa
Research Area Cancer, DNA/RNA
Pathways p53 Signaling, Apoptosis, DNA Damage Repair

Application Details

Product Details anti-ATM Antibody Target Details ATM Handling References for anti-ATM Antibody (ABIN2688845) Images back to top
Application Notes Confirmed by Bioimaging: Human Confirmed by western blot: Mouse
Restrictions For Research Use only

Handling

Product Details anti-ATM Antibody Target Details ATM Application Details References for anti-ATM Antibody (ABIN2688845) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-ATM Antibody (ABIN2688845)

Product Details anti-ATM Antibody Target Details ATM Application Details Handling Images back to top
Product cited in:

Pellegrini, Celeste, Difilippantonio, Guo, Wang, Feigenbaum, Nussenzweig: "Autophosphorylation at serine 1987 is dispensable for murine Atm activation in vivo." in: Nature, Vol. 443, Issue 7108, pp. 222-5, 2006

Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, 2003