anti-Mouse (Murine) ATM antibody for Flow Cytometry

Recommended ATM Antibody (supplied by: Log in to see )

Antigen
Ataxia Telangiectasia Mutated (ATM) Antibodies
  • ATM
  • Atm
  • CG6535
  • Dmel\\CG6535
  • Tefu
  • atm
  • atm/tefu
  • dATM
  • tef
  • Xatm
  • at1
  • atdc
  • tel1
  • telo1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • ATE
  • TEL1
  • TELO1
  • AI256621
  • C030026E19Rik
  • telomere fusion
  • ataxia telangiectasia mutated
  • ataxia telangiectasia mutated (atm)
  • ataxia telangiectasia mutated homolog (human)
  • tefu
  • atm
  • ATM
  • EDI_100660
  • CpipJ_CPIJ001772
  • BDBG_08252
  • PAAG_02532
  • MCYG_05088
  • VDBG_06833
  • ACLA_015700
  • AaeL_AAEL014900
  • MGYG_07634
  • PGTG_14279
  • Atm
  • LOC100349299
Epitope
C-Term
89
39
17
16
16
15
8
8
6
6
6
6
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
359
157
78
27
8
1
1
1
1
Host
Rabbit
233
99
17
9
3
Clonality
Polyclonal
Conjugate
This ATM antibody is un-conjugated
12
11
10
9
6
6
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
219
125
109
87
85
71
58
44
27
21
13
6
4
4
2
2
2
1
1
Supplier
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Catalog No. ABIN151030
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.325272 ABIN2662388 FACS PE Mouse IgG1 kappa phosphorylated Log in to see 10H11-E12 3
14.325272 ABIN4282176 FACS PE Rabbit C-Term Log in to see Polyclonal
1 ABIN151775 ChIP ELISA FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 full length Log in to see 2C1 114
1 ABIN685582 FACS IF (cc) IF (p) IHC (p) Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN2808094 FACS IF (cc) IF (p) Alexa Fluor 594 Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN685585 FACS IF (cc) IF (p) Cy3 Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN685588 FACS IF (cc) IF (p) Cy7 Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN685587 FACS IF (cc) IF (p) Cy5.5 Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN685586 FACS IF (cc) IF (p) Cy5 Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN685589 FACS IF (cc) IF (p) FITC Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN884037 FACS IF (cc) IF (p) Alexa Fluor 350 Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN884038 FACS IF (cc) IF (p) Alexa Fluor 488 Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN884036 FACS IF (cc) IF (p) Alexa Fluor 555 Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN884039 FACS IF (cc) IF (p) Alexa Fluor 647 Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN2662389 FACS PE Mouse IgG1 kappa phosphorylated Log in to see 10H11-E12 3
1 ABIN4997014 FACS PE Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN4997013 FACS IF (cc) IF (p) Alexa Fluor 750 Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN4997012 FACS IF (cc) IF (p) Alexa Fluor 680 Rabbit IgG AA 2700-2750 Log in to see Polyclonal
1 ABIN363380 ICC FACS IF IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN4282181 ELISA FACS ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG1 full length Log in to see 2C1

General

Antigen Ataxia Telangiectasia Mutated (ATM) Antibodies
Epitope C-Term
(89), (39), (17), (16), (16), (15), (8), (8), (6), (6), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(359), (157), (78), (27), (8), (1), (1), (1), (1)
Host Rabbit
(233), (99), (17), (9), (3)
Clonality
Polyclonal
Conjugate This ATM antibody is un-conjugated
(12), (11), (10), (9), (6), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(219), (125), (109), (87), (85), (71), (58), (44), (27), (21), (13), (6), (4), (4), (2), (2), (2), (1), (1)
Pubmed 20 references available
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Product Details anti-ATM Antibody

Target Details ATM Application Details Handling ProductDetails: References for anti-ATM antibody (ABIN151030) Images
Purification Immunogen affinity purified
Immunogen A fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence). [UniProt# Q13315]

Target Details ATM

Product Details anti-ATM Antibody Application Details Handling ProductDetails: References for anti-ATM antibody (ABIN151030) Images back to top
Antigen
Alternative Name ATM (ATM Antibody Abstract)
Background Gene Symbol: ATM
Molecular Weight Theoretical MW: 350 kDa
Gene ID 472
UniProt Q13315
Research Area Cancer, DNA/RNA
Pathways p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus

Application Details

Product Details anti-ATM Antibody Target Details ATM Handling ProductDetails: References for anti-ATM antibody (ABIN151030) Images back to top
Application Notes Western Blot 1:500-1:1000, Flow Cytometry, ELISA, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100This ATM antibody can be used for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, it detects a band at ~350 kDa, representing ATM. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry-FFPE sections protocol specific for ATM Antibody Immunohistochemistry- FFPE sectionsDeparaffinization
1. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.
. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.Quench Endogenous Peroxidase
. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation.
. Place slides in distilled water: 2 changes for 2 minutes each.Retrieve Epitopes
. Preheat Citrate
Restrictions For Research Use only

Handling

Product Details anti-ATM Antibody Target Details ATM Application Details ProductDetails: References for anti-ATM antibody (ABIN151030) Images back to top
Format Liquid
Concentration 2.5 mg/mL
Buffer PBS and 50 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

ProductDetails: References for anti-ATM antibody (ABIN151030)

Product Details anti-ATM Antibody Target Details ATM Application Details Handling Images back to top
Product cited in:

Li, Li, Kong, Yan, Ahmad, Liu: "Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response." in: Cancer research, Vol. 77, Issue 12, pp. 3169-3180, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Wu, Wang, Chen, Chao, Chang, Chen, Hung: "Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity." in: Stem cells translational medicine, Vol. 6, Issue 6, pp. 1504-1514, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Qi, Qiu, Gu, Tian, Zhang: "ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts." in: Scientific reports, Vol. 6, pp. 24700, 2016 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Nagy, Kalousi, Furst, Koch, Fischer, Soutoglou: "Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs." in: PLoS genetics, Vol. 12, Issue 2, pp. e1005791, 2016 Method employed by authors: Western Blotting (WB)

Parplys, Zhao, Sharma, Groesser, Liang, Maranon, Leung, Grundt, Dray, Idate, Østvold, Schild, Sung, Wiese: "NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability." in: Nucleic acids research, Vol. 43, Issue 20, pp. 9817-34, 2015 (Sample species: Human). Further details: Western Blotting

Murata, Uehara, Hosoi: "Activation of mTORC1 under nutrient starvation conditions increases cellular radiosensitivity in human liver cancer cell lines, HepG2 and HuH6." in: Biochemical and biophysical research communications, Vol. 468, Issue 4, pp. 684-90, 2015 (Sample species: Human). Further details: Western Blotting

Yoon, Ishdorj, Graham, Johnston, Gibson: "Valproic acid enhances fludarabine-induced apoptosis mediated by ROS and involving decreased AKT and ATM activation in B-cell-lymphoid neoplastic cells." in: Apoptosis : an international journal on programmed cell death, Vol. 19, Issue 1, pp. 191-200, 2014 (Sample species: Human). Further details: Western Blotting

Morita, Tanimoto, Murakami, Morinaga, Hosoi: "Mitochondria are required for ATM activation by extranuclear oxidative stress in cultured human hepatoblastoma cell line Hep G2 cells." in: Biochemical and biophysical research communications, Vol. 443, Issue 4, pp. 1286-90, 2014 (Sample species: Human). Further details: Western Blotting

Silva, Stambaugh, Yokomori, Shah, Berns: "DNA damage to a single chromosome end delays anaphase onset." in: The Journal of biological chemistry, Vol. 289, Issue 33, pp. 22771-84, 2014

Brinley, Theriot, Nelman-Gonzalez, Crucian, Stowe, Barrett, Pierson: "Characterization of Epstein-Barr virus reactivation in a modeled spaceflight system." in: Journal of cellular biochemistry, Vol. 114, Issue 3, pp. 616-24, 2013 (Sample species: Human). Further details: Flow Cytometry

Gautam, Bridge: "The kinase activity of ataxia-telangiectasia mutated interferes with adenovirus E4 mutant DNA replication." in: Journal of virology, Vol. 87, Issue 15, pp. 8687-96, 2013 (Sample species: Human). Further details: Western Blotting

Rankin, Sarkar, Faller: "Mechanism of T-oligo-induced cell cycle arrest in Mia-PaCa pancreatic cancer cells." in: Journal of cellular physiology, Vol. 227, Issue 6, pp. 2586-94, 2012 (Sample species: Human). Further details: Western Blotting

Jacquemin, Rieunier, Jacob, Bellanger, dEnghien, Laugé, Stoppa-Lyonnet, Stern: "Underexpression and abnormal localization of ATM products in ataxia telangiectasia patients bearing ATM missense mutations." in: European journal of human genetics : EJHG, Vol. 20, Issue 3, pp. 305-12, 2012 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Nguyen, Possemato, Bauerlein, Xie, Scully, Hahn: "Nek4 regulates entry into replicative senescence and the response to DNA damage in human fibroblasts." in: Molecular and cellular biology, Vol. 32, Issue 19, pp. 3963-77, 2012 (Sample species: Human). Further details: Western Blotting

Xie, Li, Xing, Tian, Yin, He, Zhang: "ATM-mediated NuSAP phosphorylation induces mitotic arrest." in: Biochemical and biophysical research communications, Vol. 404, Issue 1, pp. 413-8, 2011 (Sample species: Human). Further details: Western Blotting

Wang, Wu, Wei, Hong, Chiang: "Inactivation of ataxia telangiectasia mutated gene can increase intracellular reactive oxygen species levels and alter radiation-induced cell death pathways in human glioma cells." in: International journal of radiation biology, Vol. 87, Issue 4, pp. 432-42, 2011 (Sample species: Human). Further details: Flow Cytometry

Dixon, Henry, Siroky, Chu, Groen, Bissler: "Cell cycle control and DNA damage response of conditionally immortalized urothelial cells." in: PLoS ONE, Vol. 6, Issue 1, pp. e16595, 2011 (Sample species: Mouse (Murine)). Further details: Western Blotting

Du, Kayali, Bertoni, Fike, Hu, Iversen, Gatti: "Arginine-rich cell-penetrating peptide dramatically enhances AMO-mediated ATM aberrant splicing correction and enables delivery to brain and cerebellum." in: Human molecular genetics, Vol. 20, Issue 16, pp. 3151-60, 2011 (Sample species: Human). Further details: Western Blotting

Yang, Chou, Pan, Chen, Tsou, Yeh, Cheung, Chen, Chang: "Enhancement of non-homologous end joining DNA repair capacity confers cancer cells resistance to the novel selenophene compound, D-501036." in: Cancer letters, Vol. 309, Issue 1, pp. 110-8, 2011 (Sample species: Human). Further details: Western Blotting

Hu, Du, Nagabayashi, Seeger, Gatti: "ATM is down-regulated by N-Myc-regulated microRNA-421." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 4, pp. 1506-11, 2010 (Sample species: Human). Further details: Western Blotting,ELISA

Images

Product Details anti-ATM Antibody Target Details ATM Application Details Handling ProductDetails: References for anti-ATM antibody (ABIN151030) back to top
Supplier Images
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Western Blot: ATM Antibody [ABIN151030] - Detection of ATM in HeLa whole cell lysate ...
Immunofluorescence (IF) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Immunohistochemical staining using ABIN151030.
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Detection of ATM in HeLa whole cell lysate using ABIN151030. (30 second exposure)
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Western Blot: ATM Antibody [ABIN151030] - Detection of ATM in Raji whole cell lysate.
Flow Cytometry (FACS) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Flow Cytometry: ATM Antibody [ABIN151030] - Analysis of PE conjugate of ABIN151030. A...
Immunohistochemistry (IHC) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Immunohistochemistry: ATM Antibody [ABIN151030] - Staining of Testis, Seminiferous Tu...
Immunofluorescence (IF) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Immunocytochemistry/Immunofluorescence: ATM Antibody [ABIN151030] - HeLa cells were f...