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anti-Human ATR antibody for Enzyme Immunoassay

Recommended ATR Antibody (supplied by: Log in to see )

Antigen
Ataxia Telangiectasia and Rad3 Related (ATR) Antibodies
  • ARABIDOPSIS THALIANA ATAXIA TELANGIECTASIA-MUTATED AND RAD3-RELATED
  • ATATR
  • Ataxia telangiectasia-mutated and RAD3-related
  • ATR
  • ATRAD3
  • FCTCS
  • frp1
  • FRP1
  • MEC1
  • mec1
  • MHK7.5
  • MHK7_5
  • SCKL
  • sckl
  • SCKL1
  • sckl1
  • si:dkey-231j24.1
  • XATR
Epitope
Internal Region
1
1
1
1
1
Reactivity
Dog (Canine), Fish, Human, Monkey, Mouse (Murine), Rat (Rattus), Xenopus laevis
8
4
2
1
Host
Rabbit
6
2
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
5
2
1
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Catalog No. ABIN401401
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000888695 ABIN614390 EIA IHC (p) Mouse IgG2a AA 2545-2645 Log in to see 1-00E-009

Top referenced anti-ATR antibody for Enzyme Immunoassay

Similar anti-ATR Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine) Fish Monkey Rat (Rattus) Xenopus laevis
Western Blotting (WB) 7 Antibodies 5 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Ataxia Telangiectasia and Rad3 Related (ATR) Antibodies
Epitope Internal Region
(1), (1), (1), (1), (1)
Reactivity Dog (Canine), Fish, Human, Monkey, Mouse (Murine), Rat (Rattus), Xenopus laevis
(8), (4), (2), (1)
Host Rabbit
(6), (2)
Clonality
Polyclonal
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(5), (2), (1)
Pubmed 5 references available
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Product Details anti-ATR Antibody

Target Details ATR Application Details Handling References for anti-ATR Antibody (ABIN401401) Images
Specificity This antibody is specific for ATR protein.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Monkey, Canine, Fish, Xenopus.
Species reactivity (tested):Human.
Purification Affinity chromatography
Immunogen Synthetic peptide corresponding to an internal region of human ATR protein
Isotype IgG

Target Details ATR

Product Details anti-ATR Antibody Application Details Handling References for anti-ATR Antibody (ABIN401401) Images back to top
Antigen
Alternative Name ATR / FRP1 (ATR Antibody Abstract)
Background Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNAPK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4). ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during Sphase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.Synonyms: Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, Serine/threonine-protein kinase ATR
Gene ID 545
NCBI Accession NP_001175
UniProt Q13535
Pathways p53 Signaling, Apoptosis, DNA Damage Repair

Application Details

Product Details anti-ATR Antibody Target Details ATR Handling References for anti-ATR Antibody (ABIN401401) Images back to top
Application Notes ELISA: 1/15,000 - 1/70,000. Western Blot: 1/1,000 - 1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ATR Antibody Target Details ATR Application Details References for anti-ATR Antibody (ABIN401401) Images back to top
Concentration 1.28 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C

References for anti-ATR Antibody (ABIN401401)

Product Details anti-ATR Antibody Target Details ATR Application Details Handling Images back to top
Background publications

Olson, Nievera, Klimovich, Fanning, Wu: "RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint." in: The Journal of biological chemistry, Vol. 281, Issue 51, pp. 39517-33, 2006

Stiff, Walker, Cerosaletti, Goodarzi, Petermann, Concannon, ODriscoll, Jeggo: "ATR-dependent phosphorylation and activation of ATM in response to UV treatment or replication fork stalling." in: The EMBO journal, Vol. 25, Issue 24, pp. 5775-82, 2006

Dellaire, Ching, Ahmed, Jalali, Tse, Bristow, Bazett-Jones: "Promyelocytic leukemia nuclear bodies behave as DNA damage sensors whose response to DNA double-strand breaks is regulated by NBS1 and the kinases ATM, Chk2, and ATR." in: The Journal of cell biology, Vol. 175, Issue 1, pp. 55-66, 2006

Bruno, De Nicola, Iezzi, Lecis, DAngelo, Di Padova, Corbi, Dimiziani, Zannini, Jekimovs, Scarsella, Porrello, Chersi, Crescenzi, Leonetti, Khanna, Soddu, Floridi, Passananti, Delia, Fanciulli: "Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint." in: Cancer cell, Vol. 10, Issue 6, pp. 473-86, 2006

Mishra, Shetty, Tang, Stuart, Byers: "The role of TGF-beta and Wnt signaling in gastrointestinal stem cells and cancer." in: Oncogene, Vol. 24, Issue 37, pp. 5775-89, 2005

Images

Product Details anti-ATR Antibody Target Details ATR Application Details Handling References for anti-ATR Antibody (ABIN401401) back to top
Supplier Images
 image for anti-ATR antibody (Ataxia Telangiectasia and Rad3 Related) (Internal Region) (ABIN401401) anti-Ataxia Telangiectasia and Rad3 Related (ATR) (Internal Region) antibody