|Application / Reactivity||Human||Mouse (Murine)||Dog (Canine)||Fish||Monkey||Rat (Rattus)||Xenopus laevis|
|Western Blotting (WB)||6 Antibodies||5 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies||2 Antibodies||1 Antibodies||1 Antibodies|
|Antigen||Ataxia Telangiectasia and Rad3 Related (ATR) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Dog (Canine), Fish, Human, Monkey, Mouse (Murine), Rat (Rattus), Xenopus laevis Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-ATR AntibodyTarget Details ATR Application Details Handling References for anti-ATR Antibody (ABIN401401) Images
|Specificity||This antibody is specific for ATR protein.|
Species reactivity (expected):Mouse, Rat, Monkey, Canine, Fish, Xenopus.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide corresponding to an internal region of human ATR protein|
Target Details ATRProduct Details anti-ATR Antibody Application Details Handling References for anti-ATR Antibody (ABIN401401) Images back to top
|Alternative Name||ATR / FRP1 (ATR Antibody Abstract)|
|Background||Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNAPK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4). ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during Sphase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.Synonyms: Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, Serine/threonine-protein kinase ATR|
|Pathways||p53 Signaling, Apoptosis, DNA Damage Repair|
Application DetailsProduct Details anti-ATR Antibody Target Details ATR Handling References for anti-ATR Antibody (ABIN401401) Images back to top
ELISA: 1/15,000 - 1/70,000. Western Blot: 1/1,000 - 1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ATR Antibody Target Details ATR Application Details References for anti-ATR Antibody (ABIN401401) Images back to top
|Concentration||1.28 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
References for anti-ATR Antibody (ABIN401401)Product Details anti-ATR Antibody Target Details ATR Application Details Handling Images back to top
Olson, Nievera, Klimovich, Fanning, Wu: "RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint." in: The Journal of biological chemistry, Vol. 281, Issue 51, pp. 39517-33, 2006
Stiff, Walker, Cerosaletti, Goodarzi, Petermann, Concannon, ODriscoll, Jeggo: "ATR-dependent phosphorylation and activation of ATM in response to UV treatment or replication fork stalling." in: The EMBO journal, Vol. 25, Issue 24, pp. 5775-82, 2006
Dellaire, Ching, Ahmed, Jalali, Tse, Bristow, Bazett-Jones: "Promyelocytic leukemia nuclear bodies behave as DNA damage sensors whose response to DNA double-strand breaks is regulated by NBS1 and the kinases ATM, Chk2, and ATR." in: The Journal of cell biology, Vol. 175, Issue 1, pp. 55-66, 2006
Bruno, De Nicola, Iezzi, Lecis, DAngelo, Di Padova, Corbi, Dimiziani, Zannini, Jekimovs, Scarsella, Porrello, Chersi, Crescenzi, Leonetti, Khanna, Soddu, Floridi, Passananti, Delia, Fanciulli: "Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint." in: Cancer cell, Vol. 10, Issue 6, pp. 473-86, 2006
Mishra, Shetty, Tang, Stuart, Byers: "The role of TGF-beta and Wnt signaling in gastrointestinal stem cells and cancer." in: Oncogene, Vol. 24, Issue 37, pp. 5775-89, 2005
ImagesProduct Details anti-ATR Antibody Target Details ATR Application Details Handling References for anti-ATR Antibody (ABIN401401) back to top