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anti-Mouse (Murine) BAI1 antibody for Flow Cytometry

Recommended BAI1 Antibody (supplied by: Log in to see )

Antigen
Brain-Specific Angiogenesis Inhibitor 1 (BAI1) Antibodies
  • B830018M07Rik
  • BAI1
  • GDAIF
  • mKIAA4089
  • R75078
Epitope
N-Term, AA 97-112, Extracellular Loop
34
11
6
4
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
79
41
19
3
1
1
1
Host
Rabbit
80
Clonality
Polyclonal
Conjugate
This BAI1 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
33
31
27
22
11
11
3
2
1
1
1
Supplier
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Catalog No. ABIN1742112
$ 678.57
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN1742111 FACS IHC WB Rabbit N-Term, AA 97-112, Extracellular Loop Log in to see Polyclonal 1
0.3068567 ABIN2159450 FACS IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.0010666476 ABIN392804 FACS IHC (p) WB Rabbit Ig AA 1537-1567, C-Term Log in to see Polyclonal 2
0.0010666476 ABIN314492 ICC FACS IF IHC (p) IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-BAI1 antibody for Flow Cytometry

Similar anti-BAI1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 2 Antibodies 8 Antibodies 27 Antibodies
Flow Cytometry (FACS) 2 Antibodies 5 Antibodies 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 6 Antibodies 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 17 Antibodies
Western Blotting (WB) 8 Antibodies 22 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 10 Antibodies

General

Antigen Brain-Specific Angiogenesis Inhibitor 1 (BAI1) Antibodies
Epitope N-Term, AA 97-112, Extracellular Loop
(34), (11), (6), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(79), (41), (19), (3), (1), (1), (1)
Host Rabbit
(80)
Clonality
Polyclonal
Conjugate This BAI1 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(33), (31), (27), (22), (11), (11), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BAI1 Antibody

Target Details BAI1 Application Details Handling References for anti-BAI1 Antibody (ABIN1742112) Images
Sequence (C)GRVRTYQFDS FLESTR
Cross-Reactivity (Details) Rat, human - identical. The antibody will recognize the intact BAI1 receptor as well as the proteolytically processed N-terminal fragment. This fragment is also known as Vasculostatin (Vstat120).
Characteristics Peptide Confirmation:
Confirmed by mass-spectrography and amino acid analysis.

Buffer after reconstitution:
Phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % NaN3.

Antibody concentration after reconstitution:
0.8 mg/mL

Preadsorption Control:
1 µg peptide per 1 µg antibody.

GPCR
Purification Affinity purified on immobilized antigen.
Immunogen Peptide corresponding to amino acid residues 97-112 of mouse BAI1. Extracellular, N-terminus.

Target Details BAI1

Product Details anti-BAI1 Antibody Application Details Handling References for anti-BAI1 Antibody (ABIN1742112) Images back to top
Antigen
Alternative Name BAI1 (BAI1 Antibody Abstract)
Background Synonyms:
Brain-specific angiogenesis inhibitor 1

The three members of the brain angiogenesis inhibitor (BaI1-3) are receptors belonging to the adhesion subfamily of G-protein coupled receptor superfamily. Like all members of GPCRs, all three BaIs have seven transmembrane domains, an intracellular C-terminal tail and extracellular N-terminus. Like other adhesion members, the N-terminus is quite large1,2. Many domains are localized to the N-terminus, various glycosylations sites are present, there is a GPCR proteolysis site, a putative hormone binding domain and thrombospondin type 1 repeats which regulate the anti-angiogenic activity of thrombospondin-12,3. The C-terminal tail interacts with PDZ-domain proteins. Unique to BaI1 is a proline-rich domain required for interacting with Src homology domains and WW domain proteins2,4. Like most adhesion GPCRs, BaI also undergo proteolysis at the N-terminus at a highly rich cystein domain2. Following autocleavage, the N-terminal fragment remains associated to the receptor. In BaI1, proteolysis yields a partly secreted 120 kDa. fragment (vasculostatin-120) or a 40 kDa. fragment both having antiangiogenic effects2,5. At the mRNA level, all BaIs are expressed in fetal and adult human brain2,6. BaI2 is detected in the human heart and skeletal muscle. BaI3 is expressed in the human heart, testis and small intestine. In mouse, both BaI2 and BaI3 are restricted to the brain2. These receptors are implicated in various diseases and disorders such as primary glioma, pulmonary adenocarcinomas, gastric and colorectal cancers2,6,7. It has been designed to recognize Bai1 from rat, mouse, and human samples.
Gene ID 107831
UniProt Q3UHD1
Research Area Cardiovascular, Angiogenesis
Pathways p53 Signaling

Application Details

Product Details anti-BAI1 Antibody Target Details BAI1 Handling References for anti-BAI1 Antibody (ABIN1742112) Images back to top
Application Notes Recommended Dilution:
WB: approx. 1:200,
IHC: approx. 1:200,
FACS: approx. 1:20
Restrictions For Research Use only

Handling

Product Details anti-BAI1 Antibody Target Details BAI1 Application Details References for anti-BAI1 Antibody (ABIN1742112) Images back to top
Format Lyophilized
Reconstitution 50 µL deionized water.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
Storage 4 °C/-20 °C
Storage Comment The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, small aliquots should be stored at -20 °C or below. The further dilutions should be made using a carrier protein such as BSA (1 %).

References for anti-BAI1 Antibody (ABIN1742112)

Product Details anti-BAI1 Antibody Target Details BAI1 Application Details Handling Images back to top
Background publications

Cork, Van Meir: "Emerging roles for the BAI1 protein family in the regulation of phagocytosis, synaptogenesis, neurovasculature, and tumor development." in: Journal of molecular medicine (Berlin, Germany), Vol. 89, Issue 8, pp. 743-52, 2011

Images

Product Details anti-BAI1 Antibody Target Details BAI1 Application Details Handling References for anti-BAI1 Antibody (ABIN1742112) back to top
Supplier Images
Western Blotting (WB) image for anti-BAI1 antibody (Brain-Specific Angiogenesis Inhibitor 1) (N-Term) (ABIN1742112) Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:...
Western Blotting (WB) image for anti-BAI1 antibody (Brain-Specific Angiogenesis Inhibitor 1) (N-Term) (ABIN1742112) Western blot analysis of human HL-60 promyelocytic leukemia cell lysates: 1. Anti-BAI...
Immunohistochemistry (IHC) image for anti-BAI1 antibody (Brain-Specific Angiogenesis Inhibitor 1) (N-Term) (ABIN1742112) Expression of BAI1 in mouse olfactory bulb Immunohistochemical staining of mouse perf...
Flow Cytometry (FACS) image for anti-BAI1 antibody (Brain-Specific Angiogenesis Inhibitor 1) (N-Term) (ABIN1742112) Indirect flow cytometry analysis of live intact human HL-60 promyelocytic leukemia ce...