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|Antigen||Brain-Specific Angiogenesis Inhibitor 1 (BAI1) Antibodies|
|Epitope||N-Term, AA 97-112, Extracellular Loop Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BAI1 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
|1 reference available|
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Product Details anti-BAI1 AntibodyTarget Details BAI1 Application Details Handling ProductDetails: References for anti-BAI1 antibody (ABIN1742111) Images
|Cross-Reactivity (Details)||Rat, human - identical. The antibody will recognize the intact BAI1 receptor as well as the proteolytically processed N-terminal fragment. This fragment is also known as Vasculostatin (Vstat120).|
Confirmed by mass-spectrography and amino acid analysis.
Buffer after reconstitution:
Phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % NaN3.
Antibody concentration after reconstitution:
1 µg peptide per 1 µg antibody.
|Purification||Affinity purified on immobilized antigen.|
|Immunogen||Peptide corresponding to amino acid residues 97-112 of mouse BAI1. Extracellular, N-terminus.|
Target Details BAI1Product Details anti-BAI1 Antibody Application Details Handling ProductDetails: References for anti-BAI1 antibody (ABIN1742111) Images back to top
|Alternative Name||BAI1 (BAI1 Antibody Abstract)|
Brain-specific angiogenesis inhibitor 1
The three members of the brain angiogenesis inhibitor (BaI1-3) are receptors belonging to the adhesion subfamily of G-protein coupled receptor superfamily. Like all members of GPCRs, all three BaIs have seven transmembrane domains, an intracellular C-terminal tail and extracellular N-terminus. Like other adhesion members, the N-terminus is quite large1,2. Many domains are localized to the N-terminus, various glycosylations sites are present, there is a GPCR proteolysis site, a putative hormone binding domain and thrombospondin type 1 repeats which regulate the anti-angiogenic activity of thrombospondin-12,3. The C-terminal tail interacts with PDZ-domain proteins. Unique to BaI1 is a proline-rich domain required for interacting with Src homology domains and WW domain proteins2,4. Like most adhesion GPCRs, BaI also undergo proteolysis at the N-terminus at a highly rich cystein domain2. Following autocleavage, the N-terminal fragment remains associated to the receptor. In BaI1, proteolysis yields a partly secreted 120 kDa. fragment (vasculostatin-120) or a 40 kDa. fragment both having antiangiogenic effects2,5. At the mRNA level, all BaIs are expressed in fetal and adult human brain2,6. BaI2 is detected in the human heart and skeletal muscle. BaI3 is expressed in the human heart, testis and small intestine. In mouse, both BaI2 and BaI3 are restricted to the brain2. These receptors are implicated in various diseases and disorders such as primary glioma, pulmonary adenocarcinomas, gastric and colorectal cancers2,6,7. It has been designed to recognize Bai1 from rat, mouse, and human samples.
|Research Area||Cardiovascular, Angiogenesis|
Application DetailsProduct Details anti-BAI1 Antibody Target Details BAI1 Handling ProductDetails: References for anti-BAI1 antibody (ABIN1742111) Images back to top
WB: approx. 1:200,
IHC: approx. 1:200,
FACS: approx. 1:20
|Restrictions||For Research Use only|
HandlingProduct Details anti-BAI1 Antibody Target Details BAI1 Application Details ProductDetails: References for anti-BAI1 antibody (ABIN1742111) Images back to top
|Reconstitution||0.2 mL deionized water.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
|Storage||4 °C/-20 °C|
|Storage Comment||The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, small aliquots should be stored at -20 °C or below. The further dilutions should be made using a carrier protein such as BSA (1 %).|
ProductDetails: References for anti-BAI1 antibody (ABIN1742111)Product Details anti-BAI1 Antibody Target Details BAI1 Application Details Handling Images back to top
Cork, Van Meir: "Emerging roles for the BAI1 protein family in the regulation of phagocytosis, synaptogenesis, neurovasculature, and tumor development." in: Journal of molecular medicine (Berlin, Germany), Vol. 89, Issue 8, pp. 743-52, 2011
ImagesProduct Details anti-BAI1 Antibody Target Details BAI1 Application Details Handling ProductDetails: References for anti-BAI1 antibody (ABIN1742111) back to top