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anti-Cow (Bovine) BAX antibody for Western Blotting

Recommended BAX Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated X Protein (BAX) Antibodies
  • bax
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • bax-A
  • xBax
  • xlbax
  • BAX
  • BCL2L4
  • BAX-ALPHA
  • bcl2-associated X protein, a
  • BCL2-associated X protein
  • Bcl2-associated X protein
  • baxa
  • bax
  • BAX
  • Bax
  • LOC100355675
Epitope
N-Term
116
62
31
30
20
15
15
15
13
13
12
12
11
10
9
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human
504
173
161
66
25
22
18
13
8
2
2
1
1
1
Host
Rabbit
326
213
4
Clonality
Polyclonal
Conjugate
This BAX antibody is un-conjugated
19
14
13
11
8
7
7
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
367
213
142
122
122
113
98
49
40
36
13
6
6
4
2
2
1
1
1
1
1
1
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Catalog No. ABIN2792125
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0238388 ABIN451743 ELISA FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 kappa N-Term Log in to see 6A7 2
3.0238388 ABIN2957872 IC IF WB Rabbit C-Term Log in to see Polyclonal
3.0238388 ABIN2707357 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
3.0238388 ABIN2705572 IC IF IHC ELISA WB Rabbit Center Log in to see Polyclonal
3.0238388 ABIN2705573 IC IF IHC WB Rabbit Center Log in to see Polyclonal
3.0238388 ABIN1585631 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1585632 IHC ELISA WB Mouse N-Term Log in to see Polyclonal
1 ABIN2477588 IF IP ELISA WB Mouse IgG1 N-Term Log in to see 2D2 4
1 ABIN2157323 WB Rabbit Log in to see Polyclonal

Top referenced anti-BAX antibody for Western Blotting

Similar anti-BAX Antibodies

Application / Reactivity Human Cow (Bovine) Dog (Canine)
Cytometry by Time of Flight (CyTOF) 4 Antibodies
Western Blotting (WB) 337 Antibodies 10 Antibodies 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 97 Antibodies 3 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 116 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 133 Antibodies 7 Antibodies 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 34 Antibodies 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies

General

Antigen BCL2-Associated X Protein (BAX) Antibodies
Epitope N-Term
(116), (62), (31), (30), (20), (15), (15), (15), (13), (13), (12), (12), (11), (10), (9), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human
(504), (173), (161), (66), (25), (22), (18), (13), (8), (2), (2), (1), (1), (1)
Host Rabbit
(326), (213), (4)
Clonality
Polyclonal
Conjugate This BAX antibody is un-conjugated
(19), (14), (13), (11), (8), (7), (7), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(367), (213), (142), (122), (122), (113), (98), (49), (40), (36), (13), (6), (6), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BAX Antibody

Target Details BAX Application Details Handling References for anti-BAX Antibody (ABIN2792125) Images
Sequence MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Human : 100 %, Pig : 100 %
Characteristics This is a rabbit polyclonal antibody against BAX. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human BAX

Target Details BAX

Product Details anti-BAX Antibody Application Details Handling References for anti-BAX Antibody (ABIN2792125) Images back to top
Antigen
Alternative Name BAX (BAX Antibody Abstract)
Background BAX encodes a protein that belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. BAX expression is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Molecular Weight 21 kDa
Gene ID 581
NCBI Accession NM_138761, NP_620116
UniProt Q07812
Research Area Apoptosis/Necrosis
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BAX Antibody Target Details BAX Handling References for anti-BAX Antibody (ABIN2792125) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 192 AA

Restrictions For Research Use only

Handling

Product Details anti-BAX Antibody Target Details BAX Application Details References for anti-BAX Antibody (ABIN2792125) Images back to top
Format Liquid
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-BAX Antibody (ABIN2792125)

Product Details anti-BAX Antibody Target Details BAX Application Details Handling Images back to top
Product cited in:

De Martino, Marfé, Longo, Fiorito, Montagnaro, Iovane, Decaro, Pagnini: "Bid cleavage, cytochrome c release and caspase activation in canine coronavirus-induced apoptosis." in: Veterinary microbiology, Vol. 141, Issue 1-2, pp. 36-45, 2010

Honda, Abe, Hiroki, Honda, Iwanuma, Yanagisawa, Ide: "Activation of caspase 3, 9, 12, and Bax in masseter muscle of mdx mice during necrosis." in: Journal of muscle research and cell motility, Vol. 28, Issue 4-5, pp. 243-7, 2007

Background publications

Yamaguchi, Chen, Bhalla, Wang: "Regulation of Bax activation and apoptotic response to microtubule-damaging agents by p53 transcription-dependent and -independent pathways." in: The Journal of biological chemistry, Vol. 279, Issue 38, pp. 39431-7, 2004

Images

Product Details anti-BAX Antibody Target Details BAX Application Details Handling References for anti-BAX Antibody (ABIN2792125) back to top
Supplier Images
Western Blotting (WB) image for anti-BAX antibody (BCL2-Associated X Protein) (N-Term) (ABIN2792125) WB Suggested Anti-BAX Antibody Titration: 1.25ug/ml
Positive Control:
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (N-Term) (ABIN2792125) anti-BCL2-Associated X Protein (BAX) (N-Term) antibody (Image 2)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BAX antibody (BCL2-Associated X Protein) (N-Term) (ABIN2792125) Rabbit Anti-BAX Antibody ,Paraffin Embedded Tissue: Human cardiac cell
Cellula...
Western Blotting (WB) image for anti-BAX antibody (BCL2-Associated X Protein) (N-Term) (ABIN2792125) Lanes: 1. Human NT-2 cells (641 µg) lysate 2. Mouse WT brain extract (841 µg)
P...
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (N-Term) (ABIN2792125) Immunohistochemistry with NT2, mouse brain; rat brain. tissue