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anti-Human BAX antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended BAX Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated X Protein (BAX) Antibodies
  • BAX
  • bax
  • bax-A
  • BAX-ALPHA
  • BCL2L4
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • xBax
  • xlbax
Epitope
AA 43-61
113
31
26
18
16
15
15
13
12
12
12
11
9
9
8
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
412
161
149
22
17
13
7
3
2
2
1
1
Host
Rabbit
307
140
4
Clonality
Polyclonal
Conjugate
This BAX antibody is un-conjugated
16
11
10
7
7
7
5
3
3
3
3
3
3
3
3
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
312
178
125
90
79
68
34
32
21
10
6
6
2
2
1
1
1
1
1
1
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Catalog No. ABIN967400
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Top referenced anti-BAX antibody for Immunohistochemistry (Formalin-fixed Sections)

Similar anti-BAX Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 171 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 78 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 121 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 63 Antibodies
Immunoprecipitation (IP) 71 Antibodies

General

Antigen BCL2-Associated X Protein (BAX) Antibodies
Epitope AA 43-61
(113), (31), (26), (18), (16), (15), (15), (13), (12), (12), (12), (11), (9), (9), (8), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(412), (161), (149), (22), (17), (13), (7), (3), (2), (2), (1), (1)
Host Rabbit
(307), (140), (4)
Clonality
Polyclonal
Conjugate This BAX antibody is un-conjugated
(16), (11), (10), (7), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(312), (178), (125), (90), (79), (68), (34), (32), (21), (10), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-BAX Antibody

Target Details BAX Application Details Handling References for anti-BAX Antibody (ABIN967400) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. This product contains thimerosal, an organic mercury compound. Mercury and mercury compounds are chemicals known to the State of California to cause birth defects or other reproductive harm. Foreseeable use of this product does not pose a known reproductive toxicity threat.
Purification The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.
Immunogen Human Bax aa. 43-61 synthetic peptide
Isotype Ig Fraction

Target Details BAX

Product Details anti-BAX Antibody Application Details Handling References for anti-BAX Antibody (ABIN967400) Images back to top
Antigen
Alternative Name Bax (BAX Antibody Abstract)
Background Bcl-2 family members are involved in mediating programmed cell death or apoptosis, and share two highly conserved functional regions, Bcl-2 homology 1 and 2 (BH1 and BH2). Several of the family members including Bcl-2 act as inhibitors of apoptosis, whereas others such as Bax promote cell death. It is thought that protein-protein interactions between Bcl-2 family members play an important role in their function. For example, Bax can homodimerize as well as heterodimerize with Bcl-2. When Bax is present in excess, it can counteract the ability of Bcl-2 to inhibit cell death. mRNA and protein studies indicate that Bax is more widely expressed than Bcl-2, suggesting that Bax has other functions besides inhibiting Bcl-2. For example, Bax has been shown to be directly induced by the tumor suppressor p53 in radiation sensitive cells, and thus may play a role in the apoptotic elimination of cells after their exposure to DNA-damaging agents. The Bax gene potentially encodes three different proteins Bax-alpha (21 kD), Bax-beta (24 kD) and Bax-gamma (5 kD). Bax-alpha is the most common transcript. The antibodies recognize human Bax. They do not cross-react with mouse Bax. A synthetic peptide corresponding to amino acids 43-61 of human Bax was used as immunogen. The specificity of the antiserum was verified by immunoprecipitation, western blot analyisis, and immunohistochemisty, as well as by comparisons with preimmune serum and by competition experiments.
Molecular Weight 21-22 kDa
Research Area Cancer, Apoptosis/Necrosis
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BAX Antibody Target Details BAX Handling References for anti-BAX Antibody (ABIN967400) Images back to top
Application Notes Applications include immunoprecipitation (1 µl/one million cells), western blot analysis (1:1000), and immunohistochemistry (1:1000) of acetone-fixed, frozen tissue sections and of formalin-fixed, paraffin-embedded tissue sections. Normal breast epithelium is suggested as a positive control for immunohistochemistry. MCF-7 human breast carcinoma cells (ATCC HTB-22) are suggested as positive controls for immunoprecipitation and western blot analysis. In western blots the antiserum detects Bax as a single band, thought to be Bax-alpha, of 21-22 kD in cell lines tested. The antiserum may also recognize Bax-beta.
Comment

Related Products: ABIN967390, ABIN968585

Restrictions For Research Use only

Handling

Product Details anti-BAX Antibody Target Details BAX Application Details References for anti-BAX Antibody (ABIN967400) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide and ≤0.03 % thimerosal
Preservative Sodium azide, Thimerosal (Merthiolate)
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-BAX Antibody (ABIN967400)

Product Details anti-BAX Antibody Target Details BAX Application Details Handling Images back to top
Product cited in:

Ohta, Iwai, Kasahara, Taniguchi, Krajewski, Reed, Miyawaki: "Immunoblot analysis of cellular expression of Bcl-2 family proteins, Bcl-2, Bax, Bcl-X and Mcl-1, in human peripheral blood and lymphoid tissues." in: International immunology, Vol. 7, Issue 11, pp. 1817-25, 1996

Krajewski, Krajewska, Shabaik, Miyashita, Wang, Reed: "Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitor of Bcl-2." in: The American journal of pathology, Vol. 145, Issue 6, pp. 1323-36, 1995

Reed: "Bcl-2 and the regulation of programmed cell death." in: The Journal of cell biology, Vol. 124, Issue 1-2, pp. 1-6, 1994

Yin, Oltvai, Korsmeyer: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." in: Nature, Vol. 369, Issue 6478, pp. 321-3, 1994

Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993

Background publications

Krajewski, Blomqvist, Franssila, Krajewska, Wasenius, Niskanen, Nordling, Reed: "Reduced expression of proapoptotic gene BAX is associated with poor response rates to combination chemotherapy and shorter survival in women with metastatic breast adenocarcinoma." in: Cancer research, Vol. 55, Issue 19, pp. 4471-8, 1995