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anti-Human BAX antibody for Western Blotting

Recommended BAX Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated X Protein (BAX) Antibodies
  • bax
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • bax-A
  • xBax
  • xlbax
  • BAX
  • BCL2L4
  • BAX-ALPHA
  • bcl2-associated X protein, a
  • BCL2-associated X protein
  • Bcl2-associated X protein
  • baxa
  • bax
  • BAX
  • Bax
  • LOC100355675
Epitope
AA 41-76, BH3 Domain
105
66
31
23
21
20
15
15
13
12
11
10
9
9
8
8
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
503
222
207
90
46
34
28
27
22
9
8
6
3
3
2
1
1
1
1
Host
Rabbit
323
211
4
Clonality
Polyclonal
Conjugate
This BAX antibody is un-conjugated
19
14
13
11
8
7
7
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
386
214
144
133
121
116
97
45
40
36
13
7
5
5
4
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN388094
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590726 ABIN222887 IP WB Rabbit IgG N-Term Log in to see Polyclonal 5
2.9590726 ABIN1534426 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
2.9590726 ABIN1532504 IF ELISA WB Rabbit IgG Log in to see Polyclonal 2
2.9590726 ABIN2792125 IHC WB Rabbit N-Term Log in to see Polyclonal 3
2.9590726 ABIN499421 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal 1
2.9590726 ABIN451743 ELISA FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 kappa N-Term Log in to see 6A7 2
2.9590726 ABIN967537 IP WB Mouse IgG1, kappa N-Term Log in to see 6A7 6
2.9590726 ABIN968295 IF IP WB Mouse IgG1 AA 55-178 Log in to see 3-Bax 5
2.9590726 ABIN968294 IF IP WB Mouse IgG1 AA 55-178 Log in to see 3 5
2.9590726 ABIN682843 IF (p) IHC (p) WB Rabbit IgG AA 179-192, pSer184 Log in to see Polyclonal 5
2.9590726 ABIN725390 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 60-110 Log in to see Polyclonal 20
2.9590726 ABIN2169481 WB Mouse IgG2a Log in to see 2B12-G6-H6
2.9590726 ABIN657135 WB Rabbit Ig AA 28-56, N-Term Log in to see Polyclonal 1
2.9590726 ABIN4283098 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 2
2.9590726 ABIN2787858 WB Rabbit N-Term Log in to see Polyclonal
2.9590726 ABIN4283102 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
2.9590726 ABIN4283096 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 2
2.9590726 ABIN1105496 WB Rabbit IgG N-Term Log in to see Polyclonal
2.9590726 ABIN2787859 WB Rabbit Middle Region Log in to see Polyclonal
2.9590726 ABIN3044317 WB Rabbit IgG AA 19-33, N-Term Log in to see Polyclonal

Top referenced anti-BAX antibody for Western Blotting

Similar anti-BAX Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 206 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 95 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 43 Antibodies
Immunofluorescence (IF) 115 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 133 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 34 Antibodies

General

Antigen BCL2-Associated X Protein (BAX) Antibodies
Epitope AA 41-76, BH3 Domain
(105), (66), (31), (23), (21), (20), (15), (15), (13), (12), (11), (10), (9), (9), (8), (8), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(503), (222), (207), (90), (46), (34), (28), (27), (22), (9), (8), (6), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(323), (211), (4)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BAX antibody is un-conjugated
(19), (14), (13), (11), (8), (7), (7), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(386), (214), (144), (133), (121), (116), (97), (45), (40), (36), (13), (7), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-BAX Antibody

Target Details BAX Application Details Handling References for anti-BAX Antibody (ABIN388094) Images
Specificity This Bax antibody was developed against a synthetic peptide between 51~81 amino acids from the N-terminal region (BH3 domain) of human Bax.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This Bax antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-76 AA from human Bax.
Clone RB0952
Isotype Ig

Target Details BAX

Product Details anti-BAX Antibody Application Details Handling References for anti-BAX Antibody (ABIN388094) Images back to top
Antigen
Alternative Name Bax (BAX Antibody Abstract)
Background Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of the Bax gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Synonyms: apoptosis regulator BAX
Molecular Weight 24220 DA
Gene ID 581
UniProt Q07814
Research Area Apoptosis/Necrosis, Cell Structure, Cancer
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BAX Antibody Target Details BAX Handling References for anti-BAX Antibody (ABIN388094) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-BAX Antibody Target Details BAX Application Details References for anti-BAX Antibody (ABIN388094) Images back to top
Format Liquid
Concentration 1.6 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BAX Antibody (ABIN388094)

Product Details anti-BAX Antibody Target Details BAX Application Details Handling Images back to top
Product cited in:

Li, Wang, Wang, Gao, Zhang, Lu: "Oridonin phosphate-induced autophagy effectively enhances cell apoptosis of human breast cancer cells." in: Medical oncology (Northwood, London, England), Vol. 32, Issue 1, pp. 365, 2014

Gavathiotis, Reyna, Davis, Bird, Walensky: "BH3-triggered structural reorganization drives the activation of proapoptotic BAX." in: Molecular cell, Vol. 40, Issue 3, pp. 481-92, 2010

Granado, Gangoiti, Ouro, Arana, Gómez-Muñoz: "Ceramide 1-phosphate inhibits serine palmitoyltransferase and blocks apoptosis in alveolar macrophages." in: Biochimica et biophysica acta, Vol. 1791, Issue 4, pp. 263-72, 2009

Background publications

Liu, Newland, Jia: "Bax conformational change is a crucial step for PUMA-mediated apoptosis in human leukemia." in: Biochemical and biophysical research communications, Vol. 310, Issue 3, pp. 956-62, 2003

Roucou, Guo, Zhang, Goodyer, LeBlanc: "Cytosolic prion protein is not toxic and protects against Bax-mediated cell death in human primary neurons." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 40877-81, 2003

Cao, Deng, May: "Cleavage of Bax to p18 Bax accelerates stress-induced apoptosis, and a cathepsin-like protease may rapidly degrade p18 Bax." in: Blood, Vol. 102, Issue 7, pp. 2605-14, 2003

McJilton, Van Sikes, Wescott, Wu, Foreman, Gregory, Weidner, Harris Ford, Morgan Lasater, Mohler, Terrian: "Protein kinase Cepsilon interacts with Bax and promotes survival of human prostate cancer cells." in: Oncogene, Vol. 22, Issue 39, pp. 7958-68, 2003

Bidère, Lorenzo, Carmona, Laforge, Harper, Dumont, Senik: "Cathepsin D triggers Bax activation, resulting in selective apoptosis-inducing factor (AIF) relocation in T lymphocytes entering the early commitment phase to apoptosis." in: The Journal of biological chemistry, Vol. 278, Issue 33, pp. 31401-11, 2003

Images

Product Details anti-BAX Antibody Target Details BAX Application Details Handling References for anti-BAX Antibody (ABIN388094) back to top
Supplier Images
Western Blotting (WB) image for anti-BAX antibody (BCL2-Associated X Protein) (AA 41-76) (ABIN388094) Bax Antibody BH3(ABIN388094) western blot analysis in HL-60 cell line lysates (35 µg/...
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (AA 41-76) (ABIN388094) Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Bax Antibody ...
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (AA 41-76) (ABIN388094) Flow cytometric analysis of HepG2 cells using Bax Antibody (BH3 Domain Specific)(bott...